The role of sea fish meat in the transmission of Vibrio parahaemolyticus to humans: An in-depth analysis of seasonal and species-specific variations

Background and Aim: Vibrio parahaemolyticus is a marine bacterium commonly associated with foodborne illnesses due to the consumption of contaminated seafood. Understanding its prevalence in both fish meat and human infections is crucial for public health. This study aimed to estimate the occurrence...

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Main Authors: Maged A. Al-Garadi, Dhary Alewy Almashhadany, Rasha N. Aziz, Dheyazan M. Ali Al-Qabili, Ohoud S. Alhumaidan, Hanouf Alnuwaysir, Al-Hammadi Mohammed Ali, Essam Sayed, A. M. Alabsi
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Language:English
Published: Veterinary World 2025-02-01
Series:Veterinary World
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Online Access:https://www.veterinaryworld.org/Vol.18/February-2025/10.pdf
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author Maged A. Al-Garadi
Dhary Alewy Almashhadany
Rasha N. Aziz
Dheyazan M. Ali Al-Qabili
Ohoud S. Alhumaidan
Hanouf Alnuwaysir
Al-Hammadi Mohammed Ali
Essam Sayed
A. M. Alabsi
author_facet Maged A. Al-Garadi
Dhary Alewy Almashhadany
Rasha N. Aziz
Dheyazan M. Ali Al-Qabili
Ohoud S. Alhumaidan
Hanouf Alnuwaysir
Al-Hammadi Mohammed Ali
Essam Sayed
A. M. Alabsi
author_sort Maged A. Al-Garadi
collection DOAJ
description Background and Aim: Vibrio parahaemolyticus is a marine bacterium commonly associated with foodborne illnesses due to the consumption of contaminated seafood. Understanding its prevalence in both fish meat and human infections is crucial for public health. This study aimed to estimate the occurrence of V. parahaemolyticus in human stool and fish meat samples while analyzing seasonal and species-specific variations in the Al-Hodeidah governorate. Materials and Methods: A total of 225 samples were collected, including 75 human stool samples from patients with gastrointestinal symptoms and 150 fish meat samples from five fish species commonly consumed in the region. Standard microbiological methods were used for the isolation and identification of V. parahaemolyticus, including culture on Thiosulfate–Citrate–Bile Salts–Sucrose (TCBS) agar, biochemical tests, and growth analysis in varying NaCl concentrations. Data were statistically analyzed using SPSS version 12, applying the Chi-square test for group comparisons with a significance level of p ≤ 0.05. Results: The overall occurrence of V. parahaemolyticus was 7.1%. Human stool samples had a occurrence of 6.7%, while fish meat samples had a slightly higher occurrence of 7.3%. The highest monthly occurrence in human samples was recorded in July (15.0%), while the highest fish contamination was detected in September (12.0%). Among fish species, Rastrelliger kanagurta (Bagah) had the highest contamination rate (20.0%), followed by Scomberomorus commerson (Dairak) at 13.3%, whereas no V. parahaemolyticus isolates were found in Dasyatis kuhlii (Safon) and Rachycentron canadum (Sakalah). Conclusion: The findings confirm the presence of V. parahaemolyticus in both human and fish meat samples, highlighting seasonal variations and species-specific differences. The peak occurrence in fish during warm months suggests a potential link between higher temperatures and bacterial prevalence. Improved seafood handling, monitoring, and public health awareness are essential to mitigate the risk of foodborne infections. Further research is needed to explore genetic determinants of virulence and antimicrobial resistance in local isolates.
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spelling doaj-art-75ca467e25674e7c9f3376eb2914cf4a2025-08-20T03:10:57ZengVeterinary WorldVeterinary World0972-89882231-09162025-02-0118234835410.14202/vetworld.2025.348-354The role of sea fish meat in the transmission of Vibrio parahaemolyticus to humans: An in-depth analysis of seasonal and species-specific variationsMaged A. Al-Garadi0https://orcid.org/0000-0002-0388-7669Dhary Alewy Almashhadany1https://orcid.org/0000-0003-4346-6532Rasha N. Aziz2Dheyazan M. Ali Al-Qabili3Ohoud S. Alhumaidan4Hanouf Alnuwaysir5Al-Hammadi Mohammed Ali6Essam Sayed7A. M. Alabsi8Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, Riyadh, 1145, Kingdom of Saudi Arabia.Department of Medical Laboratory Science, College of Science, Knowledge University, Erbil, 44001, Iraq.Department of Public Health and Zoonoses, Faculty of Veterinary Medicine, Thamar University, Dhamar, Yemen.Department of Public Health and Zoonoses, Faculty of Veterinary Medicine, Thamar University, Dhamar, Yemen.Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, 12372, Kingdom of Saudi Arabia.Microbiology Unit in the Central Research Laboratory, King Saud University, Riyadh, Kingdom of Saudi Arabia.Department of Microbiology, College of Veterinary Medicine, King Faisal University, Al-Ahsa, Kingdom of Saudi Arabia.Special Study Office, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, P. O. Box., 71033, United Arab Emirates.Department of Preclinical Sciences, School of Dentistry, Management and Science University, University Drive, off Persiaran Olahraga, 40100, Sha Alam, Selangor, Malaysia.Background and Aim: Vibrio parahaemolyticus is a marine bacterium commonly associated with foodborne illnesses due to the consumption of contaminated seafood. Understanding its prevalence in both fish meat and human infections is crucial for public health. This study aimed to estimate the occurrence of V. parahaemolyticus in human stool and fish meat samples while analyzing seasonal and species-specific variations in the Al-Hodeidah governorate. Materials and Methods: A total of 225 samples were collected, including 75 human stool samples from patients with gastrointestinal symptoms and 150 fish meat samples from five fish species commonly consumed in the region. Standard microbiological methods were used for the isolation and identification of V. parahaemolyticus, including culture on Thiosulfate–Citrate–Bile Salts–Sucrose (TCBS) agar, biochemical tests, and growth analysis in varying NaCl concentrations. Data were statistically analyzed using SPSS version 12, applying the Chi-square test for group comparisons with a significance level of p ≤ 0.05. Results: The overall occurrence of V. parahaemolyticus was 7.1%. Human stool samples had a occurrence of 6.7%, while fish meat samples had a slightly higher occurrence of 7.3%. The highest monthly occurrence in human samples was recorded in July (15.0%), while the highest fish contamination was detected in September (12.0%). Among fish species, Rastrelliger kanagurta (Bagah) had the highest contamination rate (20.0%), followed by Scomberomorus commerson (Dairak) at 13.3%, whereas no V. parahaemolyticus isolates were found in Dasyatis kuhlii (Safon) and Rachycentron canadum (Sakalah). Conclusion: The findings confirm the presence of V. parahaemolyticus in both human and fish meat samples, highlighting seasonal variations and species-specific differences. The peak occurrence in fish during warm months suggests a potential link between higher temperatures and bacterial prevalence. Improved seafood handling, monitoring, and public health awareness are essential to mitigate the risk of foodborne infections. Further research is needed to explore genetic determinants of virulence and antimicrobial resistance in local isolates.https://www.veterinaryworld.org/Vol.18/February-2025/10.pdffish meatfoodborne infectionseafood safetyseasonal variationvibrio parahaemolyticus
spellingShingle Maged A. Al-Garadi
Dhary Alewy Almashhadany
Rasha N. Aziz
Dheyazan M. Ali Al-Qabili
Ohoud S. Alhumaidan
Hanouf Alnuwaysir
Al-Hammadi Mohammed Ali
Essam Sayed
A. M. Alabsi
The role of sea fish meat in the transmission of Vibrio parahaemolyticus to humans: An in-depth analysis of seasonal and species-specific variations
Veterinary World
fish meat
foodborne infection
seafood safety
seasonal variation
vibrio parahaemolyticus
title The role of sea fish meat in the transmission of Vibrio parahaemolyticus to humans: An in-depth analysis of seasonal and species-specific variations
title_full The role of sea fish meat in the transmission of Vibrio parahaemolyticus to humans: An in-depth analysis of seasonal and species-specific variations
title_fullStr The role of sea fish meat in the transmission of Vibrio parahaemolyticus to humans: An in-depth analysis of seasonal and species-specific variations
title_full_unstemmed The role of sea fish meat in the transmission of Vibrio parahaemolyticus to humans: An in-depth analysis of seasonal and species-specific variations
title_short The role of sea fish meat in the transmission of Vibrio parahaemolyticus to humans: An in-depth analysis of seasonal and species-specific variations
title_sort role of sea fish meat in the transmission of vibrio parahaemolyticus to humans an in depth analysis of seasonal and species specific variations
topic fish meat
foodborne infection
seafood safety
seasonal variation
vibrio parahaemolyticus
url https://www.veterinaryworld.org/Vol.18/February-2025/10.pdf
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