Xenogeneic-free platform for the isolation and scalable expansion of human bladder smooth muscle cells
Introduction: Smooth muscle cells (SMC) play a crucial role in bladder tissue engineering strategies. Scalable, Good Manufacturing Practice (GMP)-compliant platforms are essential for producing clinically relevant cell numbers. Materials & Methods: A gamma-irradiated human platelet lysate (H...
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Elsevier
2025-06-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X25000050 |
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| author | Teresa Franchi-Mendes Marília Silva Maria Catarina Carreira Ana Luísa Cartaxo Paulo Vale Panagiotis Karakaidos Apostolos Klinakis Ana Fernandes-Platzgummer Cláudia L. da Silva |
| author_facet | Teresa Franchi-Mendes Marília Silva Maria Catarina Carreira Ana Luísa Cartaxo Paulo Vale Panagiotis Karakaidos Apostolos Klinakis Ana Fernandes-Platzgummer Cláudia L. da Silva |
| author_sort | Teresa Franchi-Mendes |
| collection | DOAJ |
| description | Introduction: Smooth muscle cells (SMC) play a crucial role in bladder tissue engineering strategies. Scalable, Good Manufacturing Practice (GMP)-compliant platforms are essential for producing clinically relevant cell numbers. Materials & Methods: A gamma-irradiated human platelet lysate (HPL) supplement was used to develop a xeno(geneic)-free process for the isolation and scalable expansion of human bladder-derived SMC. Results: Cells were isolated using an explant-based technique and expanded ex vivo, expressing typical SMC markers (α-SMA, desmin, caldesmon and SM22-α). Cell culture was successfully scaled-up using spinner flasks combined with plastic microcarriers, starting with a 2.8 × 103 cells/cm2 inoculum (i.e. 1 × 106 cells). Cell-microcarrier adhesion rates exceeded 80% within 24 hours with fold expansion ranging from 3.5 to 16.8 after 7 days, dependent on donor variability. Harvested cells retained their SMC phenotype. Conclusions: This xeno-free, GMP compliant process enables scalable manufacturing of human bladder-derived SMC while preserving cell identity, potentially advancing clinical applications in bladder engineering. |
| format | Article |
| id | doaj-art-758be9b26a41465f8e6dbfb47c702b48 |
| institution | DOAJ |
| issn | 2215-017X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj-art-758be9b26a41465f8e6dbfb47c702b482025-08-20T03:21:51ZengElsevierBiotechnology Reports2215-017X2025-06-0146e0087810.1016/j.btre.2025.e00878Xenogeneic-free platform for the isolation and scalable expansion of human bladder smooth muscle cellsTeresa Franchi-Mendes0Marília Silva1Maria Catarina Carreira2Ana Luísa Cartaxo3Paulo Vale4Panagiotis Karakaidos5Apostolos Klinakis6Ana Fernandes-Platzgummer7Cláudia L. da Silva8Department of Bioengineering and iBB - Institute for Bioengineering and Biosciences at Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal; Associate Laboratory i4HB - Institute for Health and Bioeconomy at Instituto Superior Técnico, Universidade de Lisboa, Lisboa, PortugalDepartment of Bioengineering and iBB - Institute for Bioengineering and Biosciences at Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal; Associate Laboratory i4HB - Institute for Health and Bioeconomy at Instituto Superior Técnico, Universidade de Lisboa, Lisboa, PortugalDepartment of Bioengineering and iBB - Institute for Bioengineering and Biosciences at Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal; Associate Laboratory i4HB - Institute for Health and Bioeconomy at Instituto Superior Técnico, Universidade de Lisboa, Lisboa, PortugalDepartment of Bioengineering and iBB - Institute for Bioengineering and Biosciences at Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal; Associate Laboratory i4HB - Institute for Health and Bioeconomy at Instituto Superior Técnico, Universidade de Lisboa, Lisboa, PortugalUrology Department, Hospital CUF Descobertas, Lisboa, PortugalBiomedical Research Foundation of the Academy of Athens, Athens, GreeceBiomedical Research Foundation of the Academy of Athens, Athens, GreeceDepartment of Bioengineering and iBB - Institute for Bioengineering and Biosciences at Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal; Associate Laboratory i4HB - Institute for Health and Bioeconomy at Instituto Superior Técnico, Universidade de Lisboa, Lisboa, PortugalDepartment of Bioengineering and iBB - Institute for Bioengineering and Biosciences at Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal; Associate Laboratory i4HB - Institute for Health and Bioeconomy at Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal; Corresponding author.Introduction: Smooth muscle cells (SMC) play a crucial role in bladder tissue engineering strategies. Scalable, Good Manufacturing Practice (GMP)-compliant platforms are essential for producing clinically relevant cell numbers. Materials & Methods: A gamma-irradiated human platelet lysate (HPL) supplement was used to develop a xeno(geneic)-free process for the isolation and scalable expansion of human bladder-derived SMC. Results: Cells were isolated using an explant-based technique and expanded ex vivo, expressing typical SMC markers (α-SMA, desmin, caldesmon and SM22-α). Cell culture was successfully scaled-up using spinner flasks combined with plastic microcarriers, starting with a 2.8 × 103 cells/cm2 inoculum (i.e. 1 × 106 cells). Cell-microcarrier adhesion rates exceeded 80% within 24 hours with fold expansion ranging from 3.5 to 16.8 after 7 days, dependent on donor variability. Harvested cells retained their SMC phenotype. Conclusions: This xeno-free, GMP compliant process enables scalable manufacturing of human bladder-derived SMC while preserving cell identity, potentially advancing clinical applications in bladder engineering.http://www.sciencedirect.com/science/article/pii/S2215017X25000050Bladder tissue engineeringSmooth muscle cellsXeno(geneic)-freeMicrocarrier-based expansion |
| spellingShingle | Teresa Franchi-Mendes Marília Silva Maria Catarina Carreira Ana Luísa Cartaxo Paulo Vale Panagiotis Karakaidos Apostolos Klinakis Ana Fernandes-Platzgummer Cláudia L. da Silva Xenogeneic-free platform for the isolation and scalable expansion of human bladder smooth muscle cells Biotechnology Reports Bladder tissue engineering Smooth muscle cells Xeno(geneic)-free Microcarrier-based expansion |
| title | Xenogeneic-free platform for the isolation and scalable expansion of human bladder smooth muscle cells |
| title_full | Xenogeneic-free platform for the isolation and scalable expansion of human bladder smooth muscle cells |
| title_fullStr | Xenogeneic-free platform for the isolation and scalable expansion of human bladder smooth muscle cells |
| title_full_unstemmed | Xenogeneic-free platform for the isolation and scalable expansion of human bladder smooth muscle cells |
| title_short | Xenogeneic-free platform for the isolation and scalable expansion of human bladder smooth muscle cells |
| title_sort | xenogeneic free platform for the isolation and scalable expansion of human bladder smooth muscle cells |
| topic | Bladder tissue engineering Smooth muscle cells Xeno(geneic)-free Microcarrier-based expansion |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X25000050 |
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