Structural basis of human VANGL-PRICKLE interaction
Abstract Planar cell polarity (PCP) is an evolutionarily conserved process for development and morphogenesis in metazoans. The well-organized polarity pattern in cells is established by the asymmetric distribution of two core protein complexes on opposite sides of the cell membrane. The Van Gogh-lik...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-55396-3 |
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| _version_ | 1841559289262505984 |
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| author | Yanyi Song Shuyi Jian Junlin Teng Pengli Zheng Zhe Zhang |
| author_facet | Yanyi Song Shuyi Jian Junlin Teng Pengli Zheng Zhe Zhang |
| author_sort | Yanyi Song |
| collection | DOAJ |
| description | Abstract Planar cell polarity (PCP) is an evolutionarily conserved process for development and morphogenesis in metazoans. The well-organized polarity pattern in cells is established by the asymmetric distribution of two core protein complexes on opposite sides of the cell membrane. The Van Gogh-like (VANGL)-PRICKLE (PK) pair is one of these two key regulators; however, their structural information and detailed functions have been unclear. Here, we present five cryo-electron microscopy structures of human VANGL1, VANGL2, and their complexes with PK1 at resolutions of 2.2–3.0 Å. Through biochemical and cell imaging experiments, we decipher the molecular details of the VANGL-PK interaction. Furthermore, we reveal that PK1 can target VANGL-containing intracellular vesicles to the peripheral cell membrane. These findings provide a solid foundation to understand the explicit interaction between VANGL and PK while opening new avenues for subsequent studies of the PCP pathway. |
| format | Article |
| id | doaj-art-757d02118e5e457bae16a27e50536242 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-757d02118e5e457bae16a27e505362422025-01-05T12:38:31ZengNature PortfolioNature Communications2041-17232025-01-0116111310.1038/s41467-024-55396-3Structural basis of human VANGL-PRICKLE interactionYanyi Song0Shuyi Jian1Junlin Teng2Pengli Zheng3Zhe Zhang4State Key Laboratory of Membrane Biology, Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, Academy for Advanced Interdisciplinary Studies, Peking UniversityMOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking UniversityMOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking UniversityMOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking UniversityState Key Laboratory of Membrane Biology, Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, Academy for Advanced Interdisciplinary Studies, Peking UniversityAbstract Planar cell polarity (PCP) is an evolutionarily conserved process for development and morphogenesis in metazoans. The well-organized polarity pattern in cells is established by the asymmetric distribution of two core protein complexes on opposite sides of the cell membrane. The Van Gogh-like (VANGL)-PRICKLE (PK) pair is one of these two key regulators; however, their structural information and detailed functions have been unclear. Here, we present five cryo-electron microscopy structures of human VANGL1, VANGL2, and their complexes with PK1 at resolutions of 2.2–3.0 Å. Through biochemical and cell imaging experiments, we decipher the molecular details of the VANGL-PK interaction. Furthermore, we reveal that PK1 can target VANGL-containing intracellular vesicles to the peripheral cell membrane. These findings provide a solid foundation to understand the explicit interaction between VANGL and PK while opening new avenues for subsequent studies of the PCP pathway.https://doi.org/10.1038/s41467-024-55396-3 |
| spellingShingle | Yanyi Song Shuyi Jian Junlin Teng Pengli Zheng Zhe Zhang Structural basis of human VANGL-PRICKLE interaction Nature Communications |
| title | Structural basis of human VANGL-PRICKLE interaction |
| title_full | Structural basis of human VANGL-PRICKLE interaction |
| title_fullStr | Structural basis of human VANGL-PRICKLE interaction |
| title_full_unstemmed | Structural basis of human VANGL-PRICKLE interaction |
| title_short | Structural basis of human VANGL-PRICKLE interaction |
| title_sort | structural basis of human vangl prickle interaction |
| url | https://doi.org/10.1038/s41467-024-55396-3 |
| work_keys_str_mv | AT yanyisong structuralbasisofhumanvanglprickleinteraction AT shuyijian structuralbasisofhumanvanglprickleinteraction AT junlinteng structuralbasisofhumanvanglprickleinteraction AT penglizheng structuralbasisofhumanvanglprickleinteraction AT zhezhang structuralbasisofhumanvanglprickleinteraction |