Intronic RNAscope probes enable precise identification of cardiomyocyte nuclei and cell cycle activity
Abstract Cardiac regeneration studies have been plagued by technical challenges in unequivocally identifying cardiomyocyte (CM) nuclei in cardiac sections, crucial for accurate identification of cycling CMs. The use of antibodies to sarcomeric proteins is error-prone, the CM specificity of common nu...
Saved in:
| Main Authors: | Zhe Yu, Sen Zhang, Julius Bogomolovas, Ju Chen, Sylvia M. Evans |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-04-01
|
| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-08012-z |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Nonsense-mediated decay enables intron gain in Drosophila.
by: Ashley Farlow, et al.
Published: (2010-01-01) -
Single Molecule Fluorescence In Situ Hybridization Using RNAscope to Study Hematopoietic and Vascular Interactions in the Zebrafish Embryo and Larva
by: Léa Torcq, et al.
Published: (2025-04-01) -
Prp16 enables efficient splicing of introns with diverse exonic consensus elements in the short-intron rich Cryptococcus neoformans transcriptome
by: Manendra Singh Negi, et al.
Published: (2025-12-01) -
Illuminating understudied kinases: a generalizable biosensor development method applied to protein kinase N
by: Julius Bogomolovas, et al.
Published: (2025-01-01) -
Protocol to isolate dorsal root ganglion neurons from embryonic rats by immunopanning and characterize them using RNAscope and immunofluorescence
by: Samuel Faraguna, et al.
Published: (2025-06-01)