From Target Prediction to Mechanistic Insights: Revealing Air Pollution-Driven Mechanisms in Endometrial Cancer via Interpretable Machine Learning and Molecular Docking

Air pollution is a known contributor to cancer risk, although its specific impact on endometrial cancer (EC) remains unclear. This study integrates network toxicology, transcriptomics, molecular docking, and machine learning to investigate pollutant–gene interactions in EC. We identify 83 air pollut...

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Main Authors: Hongyao Liu, Yueqing Zou
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Atmosphere
Subjects:
Online Access:https://www.mdpi.com/2073-4433/16/7/841
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author Hongyao Liu
Yueqing Zou
author_facet Hongyao Liu
Yueqing Zou
author_sort Hongyao Liu
collection DOAJ
description Air pollution is a known contributor to cancer risk, although its specific impact on endometrial cancer (EC) remains unclear. This study integrates network toxicology, transcriptomics, molecular docking, and machine learning to investigate pollutant–gene interactions in EC. We identify 83 air pollution-associated EC genes (APECGs), with TNF, ESR1, IL1B, NFKB1, and PTGS2 as the hub genes. A 13-gene RSF-SuperPC model, including CCNE1, SLC2A1, AHCY, and CDC25C, shows effective prognostic stratification. Molecular docking reveals strong binding between pollutants (e.g., benzene, toluene, and ethylbenzene) and key APECGs. The enrichment and SHAP analyses suggest that pollutant-driven EC progression involves DNA damage, metabolic reprogramming, epigenetic dysregulation, immune suppression, and inflammation. These findings reveal potential mechanisms linking air pollution to EC and support the development of biomarkers for high-exposure populations. Further experimental and epidemiological validation is needed to enable clinical translation.
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series Atmosphere
spelling doaj-art-745ad0de19f84acebdb8a34109b140752025-08-20T03:13:39ZengMDPI AGAtmosphere2073-44332025-07-0116784110.3390/atmos16070841From Target Prediction to Mechanistic Insights: Revealing Air Pollution-Driven Mechanisms in Endometrial Cancer via Interpretable Machine Learning and Molecular DockingHongyao Liu0Yueqing Zou1Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, ChinaDepartment of Business Administration, Zhejiang Institute of Administration, Hangzhou 311121, ChinaAir pollution is a known contributor to cancer risk, although its specific impact on endometrial cancer (EC) remains unclear. This study integrates network toxicology, transcriptomics, molecular docking, and machine learning to investigate pollutant–gene interactions in EC. We identify 83 air pollution-associated EC genes (APECGs), with TNF, ESR1, IL1B, NFKB1, and PTGS2 as the hub genes. A 13-gene RSF-SuperPC model, including CCNE1, SLC2A1, AHCY, and CDC25C, shows effective prognostic stratification. Molecular docking reveals strong binding between pollutants (e.g., benzene, toluene, and ethylbenzene) and key APECGs. The enrichment and SHAP analyses suggest that pollutant-driven EC progression involves DNA damage, metabolic reprogramming, epigenetic dysregulation, immune suppression, and inflammation. These findings reveal potential mechanisms linking air pollution to EC and support the development of biomarkers for high-exposure populations. Further experimental and epidemiological validation is needed to enable clinical translation.https://www.mdpi.com/2073-4433/16/7/841air pollutantendometrial cancerSHAPmolecular dockingmachine learning
spellingShingle Hongyao Liu
Yueqing Zou
From Target Prediction to Mechanistic Insights: Revealing Air Pollution-Driven Mechanisms in Endometrial Cancer via Interpretable Machine Learning and Molecular Docking
Atmosphere
air pollutant
endometrial cancer
SHAP
molecular docking
machine learning
title From Target Prediction to Mechanistic Insights: Revealing Air Pollution-Driven Mechanisms in Endometrial Cancer via Interpretable Machine Learning and Molecular Docking
title_full From Target Prediction to Mechanistic Insights: Revealing Air Pollution-Driven Mechanisms in Endometrial Cancer via Interpretable Machine Learning and Molecular Docking
title_fullStr From Target Prediction to Mechanistic Insights: Revealing Air Pollution-Driven Mechanisms in Endometrial Cancer via Interpretable Machine Learning and Molecular Docking
title_full_unstemmed From Target Prediction to Mechanistic Insights: Revealing Air Pollution-Driven Mechanisms in Endometrial Cancer via Interpretable Machine Learning and Molecular Docking
title_short From Target Prediction to Mechanistic Insights: Revealing Air Pollution-Driven Mechanisms in Endometrial Cancer via Interpretable Machine Learning and Molecular Docking
title_sort from target prediction to mechanistic insights revealing air pollution driven mechanisms in endometrial cancer via interpretable machine learning and molecular docking
topic air pollutant
endometrial cancer
SHAP
molecular docking
machine learning
url https://www.mdpi.com/2073-4433/16/7/841
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AT yueqingzou fromtargetpredictiontomechanisticinsightsrevealingairpollutiondrivenmechanismsinendometrialcancerviainterpretablemachinelearningandmoleculardocking