Isolation, characterization, and vascular potential of porcine cells in a three-dimensional decellularized liver matrix model
IntroductionThe bioengineering of solid tissues and organs to mitigate the organ donor shortage has become a critical area of research in tissue engineering and regenerative medicine, where establishing a functional vascular network is crucial, particularly for complex organs such as the lung, kidne...
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2025-08-01
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| author | Sara Morini Sara Morini Sara Morini Sandra Melitón Barbancho Álvaro Blanes Rodríguez Iris Pla-Palacín Pilar Sainz-Arnal Pilar Sainz-Arnal Natalia Sánchez-Romero Natalia Sánchez-Romero Natalia Sánchez-Romero Maria Victoria Falceto Olga Mitjana Antonio Romero Marcela Del Rio-Nechaevsky Marcela Del Rio-Nechaevsky Marcela Del Rio-Nechaevsky Maria Lourdes Bengochea Martinez Emma Olmedo Arbizu Sara Lorente Sara Lorente Angel Lanas Angel Lanas Angel Lanas Ana Fernandes-Platzgummer Ana Fernandes-Platzgummer Pedro M. Baptista Pedro M. Baptista Pedro M. Baptista Pedro M. Baptista |
| author_facet | Sara Morini Sara Morini Sara Morini Sandra Melitón Barbancho Álvaro Blanes Rodríguez Iris Pla-Palacín Pilar Sainz-Arnal Pilar Sainz-Arnal Natalia Sánchez-Romero Natalia Sánchez-Romero Natalia Sánchez-Romero Maria Victoria Falceto Olga Mitjana Antonio Romero Marcela Del Rio-Nechaevsky Marcela Del Rio-Nechaevsky Marcela Del Rio-Nechaevsky Maria Lourdes Bengochea Martinez Emma Olmedo Arbizu Sara Lorente Sara Lorente Angel Lanas Angel Lanas Angel Lanas Ana Fernandes-Platzgummer Ana Fernandes-Platzgummer Pedro M. Baptista Pedro M. Baptista Pedro M. Baptista Pedro M. Baptista |
| author_sort | Sara Morini |
| collection | DOAJ |
| description | IntroductionThe bioengineering of solid tissues and organs to mitigate the organ donor shortage has become a critical area of research in tissue engineering and regenerative medicine, where establishing a functional vascular network is crucial, particularly for complex organs such as the lung, kidney, and liver. This requires the isolation and characterization of various vascular cell types. In this quest, pigs have emerged as the preferred experimental animal model in this field, highlighting the importance of procuring and characterizing porcine vascular cells to create organs with functional vasculature for transplant. However, species-unique differences present challenges. Although some of the processes for isolating, expanding, and characterizing porcine vascular cells have been published, these are less established than those for human cells, requiring in our view and experience, additional research. Furthermore, no reliable and comprehensive models currently exist for testing vascular cell interactions in co-culture in vitro.MethodsIn this study, we developed effective methods to isolate and further characterize distinct porcine vascular cell types from various sources. We also introduced a straightforward and practical three-dimensional model for testing vascular cell co-culture, organization and function in vitro.Results and discussionThis proof-of-concept study demonstrates the potential of our co-culture strategy, employing a decellularized liver extracellular matrix disc scaffold microenvironment to assess cell interactions and vascular potential on a small scale in vitro. |
| format | Article |
| id | doaj-art-7386407cbaf24f1bbda498accfebc6c3 |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-08-01 |
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| spelling | doaj-art-7386407cbaf24f1bbda498accfebc6c32025-08-20T03:57:36ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-08-011310.3389/fbioe.2025.16259991625999Isolation, characterization, and vascular potential of porcine cells in a three-dimensional decellularized liver matrix modelSara Morini0Sara Morini1Sara Morini2Sandra Melitón Barbancho3Álvaro Blanes Rodríguez4Iris Pla-Palacín5Pilar Sainz-Arnal6Pilar Sainz-Arnal7Natalia Sánchez-Romero8Natalia Sánchez-Romero9Natalia Sánchez-Romero10Maria Victoria Falceto11Olga Mitjana12Antonio Romero13Marcela Del Rio-Nechaevsky14Marcela Del Rio-Nechaevsky15Marcela Del Rio-Nechaevsky16Maria Lourdes Bengochea Martinez17Emma Olmedo Arbizu18Sara Lorente19Sara Lorente20Angel Lanas21Angel Lanas22Angel Lanas23Ana Fernandes-Platzgummer24Ana Fernandes-Platzgummer25Pedro M. Baptista26Pedro M. Baptista27Pedro M. Baptista28Pedro M. Baptista29Department of Bioengineering and iBB – Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, PortugalAssociate Laboratory i4HB - Institute for Health and Bioeconomy at Instituto Superior Técnico, Universidade de Lisboa, Lisbon, PortugalInstituto de Investigación Sanitária de Aragón (IIS Aragón), Zaragoza, SpainInstituto de Investigación Sanitária de Aragón (IIS Aragón), Zaragoza, SpainInstituto de Investigación Sanitária de Aragón (IIS Aragón), Zaragoza, SpainInstituto de Investigación Sanitária de Aragón (IIS Aragón), Zaragoza, SpainInstituto de Investigación Sanitária de Aragón (IIS Aragón), Zaragoza, SpainCytes Biotechnologies SL, Barcelona, SpainInstituto de Investigación Sanitária de Aragón (IIS Aragón), Zaragoza, SpainCytes Biotechnologies SL, Barcelona, SpainFacultad de Ciencias de la Salud, Universidad San Jorge, Campus Universitario, Zaragoza, SpainDepartamento de Patología Animal, Instituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-ITA), Zaragoza, SpainDepartamento de Patología Animal, Instituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-ITA), Zaragoza, SpainDepartamento de Patología Animal, Instituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-ITA), Zaragoza, SpainBiomedical Engineering Department, Carlos III University of Madrid, Madrid, SpainCIBERER, Centro de Investigación Biomédica en Red de Enfermedades Raras, Instituto de Salud Carlos III, Madrid, SpainInstituto de Investigación Sanitaria de la Fundación Jiménez Díaz, Madrid, Spain0Hospital Clínico Universitario Lozano Blesa, Zaragoza, Spain0Hospital Clínico Universitario Lozano Blesa, Zaragoza, SpainInstituto de Investigación Sanitária de Aragón (IIS Aragón), Zaragoza, Spain0Hospital Clínico Universitario Lozano Blesa, Zaragoza, SpainInstituto de Investigación Sanitária de Aragón (IIS Aragón), Zaragoza, Spain0Hospital Clínico Universitario Lozano Blesa, Zaragoza, Spain1Centro de Investigación Biomédica en Red en el Área Temática de Enfermedades Hepáticas (CIBERehd), Madrid, SpainDepartment of Bioengineering and iBB – Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, PortugalAssociate Laboratory i4HB - Institute for Health and Bioeconomy at Instituto Superior Técnico, Universidade de Lisboa, Lisbon, PortugalInstituto de Investigación Sanitária de Aragón (IIS Aragón), Zaragoza, SpainBiomedical Engineering Department, Carlos III University of Madrid, Madrid, Spain1Centro de Investigación Biomédica en Red en el Área Temática de Enfermedades Hepáticas (CIBERehd), Madrid, Spain2Fundación Agencia Aragonesa para la Investigación y el Desarrollo, Zaragoza, SpainIntroductionThe bioengineering of solid tissues and organs to mitigate the organ donor shortage has become a critical area of research in tissue engineering and regenerative medicine, where establishing a functional vascular network is crucial, particularly for complex organs such as the lung, kidney, and liver. This requires the isolation and characterization of various vascular cell types. In this quest, pigs have emerged as the preferred experimental animal model in this field, highlighting the importance of procuring and characterizing porcine vascular cells to create organs with functional vasculature for transplant. However, species-unique differences present challenges. Although some of the processes for isolating, expanding, and characterizing porcine vascular cells have been published, these are less established than those for human cells, requiring in our view and experience, additional research. Furthermore, no reliable and comprehensive models currently exist for testing vascular cell interactions in co-culture in vitro.MethodsIn this study, we developed effective methods to isolate and further characterize distinct porcine vascular cell types from various sources. We also introduced a straightforward and practical three-dimensional model for testing vascular cell co-culture, organization and function in vitro.Results and discussionThis proof-of-concept study demonstrates the potential of our co-culture strategy, employing a decellularized liver extracellular matrix disc scaffold microenvironment to assess cell interactions and vascular potential on a small scale in vitro.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1625999/fullmesenchymal stromal cellssmooth muscle cellsendothelial cellsdecellularized liver ECM3D dECM disc vascular model |
| spellingShingle | Sara Morini Sara Morini Sara Morini Sandra Melitón Barbancho Álvaro Blanes Rodríguez Iris Pla-Palacín Pilar Sainz-Arnal Pilar Sainz-Arnal Natalia Sánchez-Romero Natalia Sánchez-Romero Natalia Sánchez-Romero Maria Victoria Falceto Olga Mitjana Antonio Romero Marcela Del Rio-Nechaevsky Marcela Del Rio-Nechaevsky Marcela Del Rio-Nechaevsky Maria Lourdes Bengochea Martinez Emma Olmedo Arbizu Sara Lorente Sara Lorente Angel Lanas Angel Lanas Angel Lanas Ana Fernandes-Platzgummer Ana Fernandes-Platzgummer Pedro M. Baptista Pedro M. Baptista Pedro M. Baptista Pedro M. Baptista Isolation, characterization, and vascular potential of porcine cells in a three-dimensional decellularized liver matrix model Frontiers in Bioengineering and Biotechnology mesenchymal stromal cells smooth muscle cells endothelial cells decellularized liver ECM 3D dECM disc vascular model |
| title | Isolation, characterization, and vascular potential of porcine cells in a three-dimensional decellularized liver matrix model |
| title_full | Isolation, characterization, and vascular potential of porcine cells in a three-dimensional decellularized liver matrix model |
| title_fullStr | Isolation, characterization, and vascular potential of porcine cells in a three-dimensional decellularized liver matrix model |
| title_full_unstemmed | Isolation, characterization, and vascular potential of porcine cells in a three-dimensional decellularized liver matrix model |
| title_short | Isolation, characterization, and vascular potential of porcine cells in a three-dimensional decellularized liver matrix model |
| title_sort | isolation characterization and vascular potential of porcine cells in a three dimensional decellularized liver matrix model |
| topic | mesenchymal stromal cells smooth muscle cells endothelial cells decellularized liver ECM 3D dECM disc vascular model |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1625999/full |
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