Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy
Aim. To investigate the changes in the maternal immune system at term pregnancy, we studied the expression of natural cytotoxicity receptors (NCRs) and the cytokine production of NK cells in term placenta decidua and peripheral blood. Methods. Term decidua and peripheral blood were taken from patien...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Pregnancy |
| Online Access: | http://dx.doi.org/10.1155/2018/4382084 |
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| _version_ | 1832549942159212544 |
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| author | Hideki Takahashi Tatsuo Yamamoto Motomi Yamazaki Takayuki Murase Takayuki Matsuno Fumihisa Chishima |
| author_facet | Hideki Takahashi Tatsuo Yamamoto Motomi Yamazaki Takayuki Murase Takayuki Matsuno Fumihisa Chishima |
| author_sort | Hideki Takahashi |
| collection | DOAJ |
| description | Aim. To investigate the changes in the maternal immune system at term pregnancy, we studied the expression of natural cytotoxicity receptors (NCRs) and the cytokine production of NK cells in term placenta decidua and peripheral blood. Methods. Term decidua and peripheral blood were taken from patients undergoing elective cesarean section. The lymphocytes were separated using density gradient centrifugation (DGC) from peripheral blood and were separated from decidua using DGC after enzyme digestion. These cells were stained with FITC anti-CD56 and Per-CP anti-CD3 monoclonal antibodies, and the NCRs were stained with PE-conjugated anti-NKG2D, NKp46, NKp30, and NKp44 monoclonal antibodies. Cytokines, including IFN-γ, TNF-α, IL-10, and TGF-β, were stained and then analyzed by flow cytometry. Results. There were fewer cells positive for NKG2D, NKp46, and NKp30 among CD56+CD3- cells in deciduas than in peripheral blood, but the percentages of NKp44-positive cells in CD56+CD3- lymphocytes in deciduas tended to be higher. Conclusion. The decreased expression of some NCRs in deciduas may be related to decreased cytotoxicity at term pregnancy, but the increased expression of NKp44 may affect the increased cytokine production in the decidua. Similarly, the expression of NCRs in the decidua may be connected to the maintenance of pregnancy at term. |
| format | Article |
| id | doaj-art-737b681a27db40e49db123497fc2a2e8 |
| institution | Kabale University |
| issn | 2090-2727 2090-2735 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Pregnancy |
| spelling | doaj-art-737b681a27db40e49db123497fc2a2e82025-02-03T06:08:06ZengWileyJournal of Pregnancy2090-27272090-27352018-01-01201810.1155/2018/43820844382084Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal PregnancyHideki Takahashi0Tatsuo Yamamoto1Motomi Yamazaki2Takayuki Murase3Takayuki Matsuno4Fumihisa Chishima5Department of Obstetrics & Gynecology, School of Medicine, Nihon University, Tokyo, JapanDepartment of Obstetrics & Gynecology, School of Medicine, Nihon University, Tokyo, JapanDepartment of Obstetrics & Gynecology, School of Medicine, Nihon University, Tokyo, JapanDepartment of Obstetrics & Gynecology, School of Medicine, Nihon University, Tokyo, JapanDepartment of Obstetrics & Gynecology, School of Medicine, Nihon University, Tokyo, JapanDepartment of Obstetrics & Gynecology, School of Medicine, Nihon University, Tokyo, JapanAim. To investigate the changes in the maternal immune system at term pregnancy, we studied the expression of natural cytotoxicity receptors (NCRs) and the cytokine production of NK cells in term placenta decidua and peripheral blood. Methods. Term decidua and peripheral blood were taken from patients undergoing elective cesarean section. The lymphocytes were separated using density gradient centrifugation (DGC) from peripheral blood and were separated from decidua using DGC after enzyme digestion. These cells were stained with FITC anti-CD56 and Per-CP anti-CD3 monoclonal antibodies, and the NCRs were stained with PE-conjugated anti-NKG2D, NKp46, NKp30, and NKp44 monoclonal antibodies. Cytokines, including IFN-γ, TNF-α, IL-10, and TGF-β, were stained and then analyzed by flow cytometry. Results. There were fewer cells positive for NKG2D, NKp46, and NKp30 among CD56+CD3- cells in deciduas than in peripheral blood, but the percentages of NKp44-positive cells in CD56+CD3- lymphocytes in deciduas tended to be higher. Conclusion. The decreased expression of some NCRs in deciduas may be related to decreased cytotoxicity at term pregnancy, but the increased expression of NKp44 may affect the increased cytokine production in the decidua. Similarly, the expression of NCRs in the decidua may be connected to the maintenance of pregnancy at term.http://dx.doi.org/10.1155/2018/4382084 |
| spellingShingle | Hideki Takahashi Tatsuo Yamamoto Motomi Yamazaki Takayuki Murase Takayuki Matsuno Fumihisa Chishima Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy Journal of Pregnancy |
| title | Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy |
| title_full | Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy |
| title_fullStr | Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy |
| title_full_unstemmed | Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy |
| title_short | Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy |
| title_sort | natural cytotoxicity receptors in decidua natural killer cells of term normal pregnancy |
| url | http://dx.doi.org/10.1155/2018/4382084 |
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