Protocol for assessing the clogging of the mitochondrial translocase of the outer membrane by precursor proteins in human cells
Summary: Protein import into the mitochondria is required for organellar function. Inefficient import can result in the stalling of mitochondrial precursors inside the translocase of the outer membrane (TOM) and blockage of the mitochondrial entry gate. Here, we present a protocol to assess the clog...
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Main Authors: | John Kim, Hilla Weidberg |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2025-03-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000231 |
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