Effects of Calcium Chloride Crosslinking Solution Concentration on the Long-Term Cell Viability of 16HBE14o- Human Bronchial Cells Embedded in Alginate-Based Hydrogels
In this preliminary study, the long-term effects of calcium chloride crosslinking concentration on viability of 16HBE14o- human bronchial epithelial cells embedded in alginate-extracellular matrix (ECM) or alginate–methylcellulose–ECM hydrogels have been investigated. There is currently a limited un...
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2025-01-01
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| author | Nathan Wood Esther I. Doria Taieba Tuba Rahman Wanhe Li Zhijian Pei Hongmin Qin |
| author_facet | Nathan Wood Esther I. Doria Taieba Tuba Rahman Wanhe Li Zhijian Pei Hongmin Qin |
| author_sort | Nathan Wood |
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| description | In this preliminary study, the long-term effects of calcium chloride crosslinking concentration on viability of 16HBE14o- human bronchial epithelial cells embedded in alginate-extracellular matrix (ECM) or alginate–methylcellulose–ECM hydrogels have been investigated. There is currently a limited understanding regarding the effects of crosslinking solution concentration on lung epithelial cells embedded in hydrogel. Furthermore, the effects of calcium chloride concentration in crosslinking solutions on other cell types have not been reported regarding whether the addition of viscosity and stiffness tuning agents such as methylcellulose will alter the responses of cells to changes in calcium chloride concentration in crosslinking solutions. While there were no significant effects of calcium chloride concentration on cell viability in alginate–ECM hydrogels, there is a decrease in cell viability in alginate–methylcellulose–ECM hydrogels crosslinked with 300 mM calcium chloride crosslinking solution. These findings have implications in the maintenance of 16HBE14o- 3D cultures with respect to the gelation of alginate with high concentrations of ionic crosslinking solution. |
| format | Article |
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| institution | Kabale University |
| issn | 2313-7673 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Biomimetics |
| spelling | doaj-art-72b9c8df08d346a2b91d8942c2a45dbf2025-01-24T13:24:41ZengMDPI AGBiomimetics2313-76732025-01-011014010.3390/biomimetics10010040Effects of Calcium Chloride Crosslinking Solution Concentration on the Long-Term Cell Viability of 16HBE14o- Human Bronchial Cells Embedded in Alginate-Based HydrogelsNathan Wood0Esther I. Doria1Taieba Tuba Rahman2Wanhe Li3Zhijian Pei4Hongmin Qin5Department of Biology, Texas A&M University, 3258 TAMU, College Station, TX 77843, USADepartment of Biology, Texas A&M University, 3258 TAMU, College Station, TX 77843, USADepartment of Industrial & Systems Engineering, Texas A&M University, 3127 TAMU, College Station, TX 77843, USADepartment of Biology, Texas A&M University, 3258 TAMU, College Station, TX 77843, USADepartment of Industrial & Systems Engineering, Texas A&M University, 3127 TAMU, College Station, TX 77843, USADepartment of Biology, Texas A&M University, 3258 TAMU, College Station, TX 77843, USAIn this preliminary study, the long-term effects of calcium chloride crosslinking concentration on viability of 16HBE14o- human bronchial epithelial cells embedded in alginate-extracellular matrix (ECM) or alginate–methylcellulose–ECM hydrogels have been investigated. There is currently a limited understanding regarding the effects of crosslinking solution concentration on lung epithelial cells embedded in hydrogel. Furthermore, the effects of calcium chloride concentration in crosslinking solutions on other cell types have not been reported regarding whether the addition of viscosity and stiffness tuning agents such as methylcellulose will alter the responses of cells to changes in calcium chloride concentration in crosslinking solutions. While there were no significant effects of calcium chloride concentration on cell viability in alginate–ECM hydrogels, there is a decrease in cell viability in alginate–methylcellulose–ECM hydrogels crosslinked with 300 mM calcium chloride crosslinking solution. These findings have implications in the maintenance of 16HBE14o- 3D cultures with respect to the gelation of alginate with high concentrations of ionic crosslinking solution.https://www.mdpi.com/2313-7673/10/1/403D culturealginatecrosslinkingepitheliamethylcelluloseviability |
| spellingShingle | Nathan Wood Esther I. Doria Taieba Tuba Rahman Wanhe Li Zhijian Pei Hongmin Qin Effects of Calcium Chloride Crosslinking Solution Concentration on the Long-Term Cell Viability of 16HBE14o- Human Bronchial Cells Embedded in Alginate-Based Hydrogels Biomimetics 3D culture alginate crosslinking epithelia methylcellulose viability |
| title | Effects of Calcium Chloride Crosslinking Solution Concentration on the Long-Term Cell Viability of 16HBE14o- Human Bronchial Cells Embedded in Alginate-Based Hydrogels |
| title_full | Effects of Calcium Chloride Crosslinking Solution Concentration on the Long-Term Cell Viability of 16HBE14o- Human Bronchial Cells Embedded in Alginate-Based Hydrogels |
| title_fullStr | Effects of Calcium Chloride Crosslinking Solution Concentration on the Long-Term Cell Viability of 16HBE14o- Human Bronchial Cells Embedded in Alginate-Based Hydrogels |
| title_full_unstemmed | Effects of Calcium Chloride Crosslinking Solution Concentration on the Long-Term Cell Viability of 16HBE14o- Human Bronchial Cells Embedded in Alginate-Based Hydrogels |
| title_short | Effects of Calcium Chloride Crosslinking Solution Concentration on the Long-Term Cell Viability of 16HBE14o- Human Bronchial Cells Embedded in Alginate-Based Hydrogels |
| title_sort | effects of calcium chloride crosslinking solution concentration on the long term cell viability of 16hbe14o human bronchial cells embedded in alginate based hydrogels |
| topic | 3D culture alginate crosslinking epithelia methylcellulose viability |
| url | https://www.mdpi.com/2313-7673/10/1/40 |
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