Identifying the best reference gene for RT-qPCR analyses of the three-dimensional osteogenic differentiation of human induced pluripotent stem cells

Identifying the best reference gene for RT-qPCR analyses of the three-dimensional osteogenic differentiation of human induced pluripotent stem cells

Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is an essential tool for gene expression analysis; choosing appropriate reference genes for normalization is crucial to ensure data reliability. However, most studies on osteogenic differentiation have had limited succe...

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Main Authors: Masakazu Okamoto, Yusuke Inagaki, Kensuke Okamura, Yoshinobu Uchihara, Kenichiro Saito, Akihito Kawai, Munehiro Ogawa, Akira Kido, Eiichiro Mori, Yasuhito Tanaka
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Bone Reports
Subjects:
hiPSCs
Osteogenic differentiation
Reference gene
Regenerative medicine
RT-qPCR
Online Access:http://www.sciencedirect.com/science/article/pii/S2352187224000834
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