Development and performance evaluation of a multiplex fluorescent PCR method for the detection of fastidious pathogens causing respiratory infections in children

Development and performance evaluation of a multiplex fluorescent PCR method for the detection of fastidious pathogens causing respiratory infections in children

Introduction: This study was performed to develop a multiplex fluorescent PCR method for the concurrent detection of six fastidious bacteria associated with respiratory tract infections. These bacteria include Streptococcus pneumoniae（SPN）, Bordetella pertussis (BP), Neisseria meningitidis (NM), Le...

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Bibliographic Details
Main Authors: Yijun Zhu, Jingchao Shi, Shihang Zhang, Weixian Yang, Xiaoyun Shan
Format: Article
Language:English
Published: The Journal of Infection in Developing Countries 2025-04-01
Series:Journal of Infection in Developing Countries
Subjects:
Respiratory infections
child
fastidious bacterium
multiplex fluorescent PCR
SYBR green
melting curve
Online Access:https://jidc.org/index.php/journal/article/view/20282
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https://jidc.org/index.php/journal/article/view/20282

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