Isolation of Full-Length RNA from a Thermophilic Cyanobacterium
Isolation of full-length mRNA without degradation is critical in the study of in vivo gene regulation and transcription, cDNA synthesis and reverse transcription (RT)-PCR. It is particularly difficult to isolate full-length mRNA from thermophiles, which have higher turnover rates of mRNA degradation...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1997-11-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/97235st08 |
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