Improving panicle blast resistance and fragrance in a high-quality japonica rice variety through breeding
IntroductionHuruan1212 (HR1212) is well-regarded for its superior eating and cooking quality in the lower reaches of the Yangtze River in China. Still, its high susceptibility to rice panicle blast and lack of fragrance have limited its further spread and utilization. Pigm and Pi-ta are two dominant...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1507827/full |
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| author | Junhua Ye Junhua Ye Kai Wang Yi Wang Zhipeng Zhao Ying Yan Hang Yang Lixia Zhang Lixia Zhang Zejun Hu Zejun Hu Zhenying Shi Dapeng Sun Jianjiang Bai Liming Cao Liming Cao Shujun Wu Shujun Wu |
| author_facet | Junhua Ye Junhua Ye Kai Wang Yi Wang Zhipeng Zhao Ying Yan Hang Yang Lixia Zhang Lixia Zhang Zejun Hu Zejun Hu Zhenying Shi Dapeng Sun Jianjiang Bai Liming Cao Liming Cao Shujun Wu Shujun Wu |
| author_sort | Junhua Ye |
| collection | DOAJ |
| description | IntroductionHuruan1212 (HR1212) is well-regarded for its superior eating and cooking quality in the lower reaches of the Yangtze River in China. Still, its high susceptibility to rice panicle blast and lack of fragrance have limited its further spread and utilization. Pigm and Pi-ta are two dominant genes known for their stable broad-spectrum resistance against rice blast fungus Magnaporthe oryzae, while badh2 is the crucial gene that regulates rice aroma.MethodsIn this study, we utilized a molecular marker-assisted selection backcrossing strategy to introduce Pigm, Pi-ta, and badh2 into introgressed lines employing re-sequencing for precise genetic background selection.ResultsFinally, we selected three introgressed lines, including two that carry Pigm with the highest background recovery rates, showing eating and cooking qualities similar to those of HR1212, and one line that pyramids Pigm, Pi-ta, and badh2, which features a strong aroma. They all displayed significantly enhanced resistance to panicle blast and improved yield compared to HR1212.DiscussionIn conclusion, this study expanded the germplasm resources of japonica, providing a material foundation for enhancing breeding programs aimed at developing rice blast-resistant and high-quality fragrant japonica varieties. Additionally, the study demonstrated that integrating molecular markers and re-sequencing can inform breeders’ decision-making more precisely and efficiently. |
| format | Article |
| id | doaj-art-7197841e7a82458fa9a2fbc811c4c976 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-7197841e7a82458fa9a2fbc811c4c9762025-01-13T06:11:00ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.15078271507827Improving panicle blast resistance and fragrance in a high-quality japonica rice variety through breedingJunhua Ye0Junhua Ye1Kai Wang2Yi Wang3Zhipeng Zhao4Ying Yan5Hang Yang6Lixia Zhang7Lixia Zhang8Zejun Hu9Zejun Hu10Zhenying Shi11Dapeng Sun12Jianjiang Bai13Liming Cao14Liming Cao15Shujun Wu16Shujun Wu17Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaShanghai Agricultural Products Preservation Processing Engineering Technology Research Center, Shanghai, ChinaKey Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaZhongken Seed Industry Co., Ltd., Shanghai, ChinaShanghai Agricultural Science and Technology Service Center, Shanghai, ChinaKey Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaKey Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaKey Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaShanghai Agricultural Products Preservation Processing Engineering Technology Research Center, Shanghai, ChinaKey Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaShanghai Agricultural Products Preservation Processing Engineering Technology Research Center, Shanghai, ChinaKey Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaKey Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaKey Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaKey Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaShanghai Agricultural Products Preservation Processing Engineering Technology Research Center, Shanghai, ChinaKey Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, ChinaShanghai Agricultural Products Preservation Processing Engineering Technology Research Center, Shanghai, ChinaIntroductionHuruan1212 (HR1212) is well-regarded for its superior eating and cooking quality in the lower reaches of the Yangtze River in China. Still, its high susceptibility to rice panicle blast and lack of fragrance have limited its further spread and utilization. Pigm and Pi-ta are two dominant genes known for their stable broad-spectrum resistance against rice blast fungus Magnaporthe oryzae, while badh2 is the crucial gene that regulates rice aroma.MethodsIn this study, we utilized a molecular marker-assisted selection backcrossing strategy to introduce Pigm, Pi-ta, and badh2 into introgressed lines employing re-sequencing for precise genetic background selection.ResultsFinally, we selected three introgressed lines, including two that carry Pigm with the highest background recovery rates, showing eating and cooking qualities similar to those of HR1212, and one line that pyramids Pigm, Pi-ta, and badh2, which features a strong aroma. They all displayed significantly enhanced resistance to panicle blast and improved yield compared to HR1212.DiscussionIn conclusion, this study expanded the germplasm resources of japonica, providing a material foundation for enhancing breeding programs aimed at developing rice blast-resistant and high-quality fragrant japonica varieties. Additionally, the study demonstrated that integrating molecular markers and re-sequencing can inform breeders’ decision-making more precisely and efficiently.https://www.frontiersin.org/articles/10.3389/fpls.2024.1507827/fullpyramidingpanicle blast resistancefragranceeating and cooking qualityre-sequencingrice |
| spellingShingle | Junhua Ye Junhua Ye Kai Wang Yi Wang Zhipeng Zhao Ying Yan Hang Yang Lixia Zhang Lixia Zhang Zejun Hu Zejun Hu Zhenying Shi Dapeng Sun Jianjiang Bai Liming Cao Liming Cao Shujun Wu Shujun Wu Improving panicle blast resistance and fragrance in a high-quality japonica rice variety through breeding Frontiers in Plant Science pyramiding panicle blast resistance fragrance eating and cooking quality re-sequencing rice |
| title | Improving panicle blast resistance and fragrance in a high-quality japonica rice variety through breeding |
| title_full | Improving panicle blast resistance and fragrance in a high-quality japonica rice variety through breeding |
| title_fullStr | Improving panicle blast resistance and fragrance in a high-quality japonica rice variety through breeding |
| title_full_unstemmed | Improving panicle blast resistance and fragrance in a high-quality japonica rice variety through breeding |
| title_short | Improving panicle blast resistance and fragrance in a high-quality japonica rice variety through breeding |
| title_sort | improving panicle blast resistance and fragrance in a high quality japonica rice variety through breeding |
| topic | pyramiding panicle blast resistance fragrance eating and cooking quality re-sequencing rice |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1507827/full |
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