Pre-column dervatization of amino acids from nigella sativa L seed hydrolysates by reversed phase HPLC

A rapid and sensitive method for analysis of amino acid hydrolysates of nigella sativa L seed has been developed using O-phthaldialehyde(OPA ) as a pre-column derivatizing agent. OPA reagents in the presence of mercaptoethanol react rapidly with primary amino acids ( less than 60 sec.) to form isin...

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Bibliographic Details
Main Author: Baghdad Science Journal
Format: Article
Language:English
Published: University of Baghdad, College of Science for Women 2008-03-01
Series:مجلة بغداد للعلوم
Online Access:http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/874
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