Determination of the physicochemical characteristics and bioactive compounds of the miracle fruit (Synsepalum dulcificum) considering different extraction and preservation methods

The miracle fruit (Synsepalum dulcificum) is distinguished by its unique ability to alter taste perceptions, making it a low-calorie sugar substitute. This study aimed to determine its physicochemical characteristics and bioactive compounds by evaluating various extraction and preservation methods....

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Main Authors: Juan Alejandro Neira Mosquera, Sungey Naynee Sánchez Llaguno, Karol Yannela Revilla Escobar, Jhonnatan Placido Aldas Morejon, Angie Estefania Iguasnia Ureta, Andy Sebastian Parrales Loor, Jonathan Alexander Arguello Cedeño
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Heliyon
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Online Access:http://www.sciencedirect.com/science/article/pii/S2405844025001744
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author Juan Alejandro Neira Mosquera
Sungey Naynee Sánchez Llaguno
Karol Yannela Revilla Escobar
Jhonnatan Placido Aldas Morejon
Angie Estefania Iguasnia Ureta
Andy Sebastian Parrales Loor
Jonathan Alexander Arguello Cedeño
author_facet Juan Alejandro Neira Mosquera
Sungey Naynee Sánchez Llaguno
Karol Yannela Revilla Escobar
Jhonnatan Placido Aldas Morejon
Angie Estefania Iguasnia Ureta
Andy Sebastian Parrales Loor
Jonathan Alexander Arguello Cedeño
author_sort Juan Alejandro Neira Mosquera
collection DOAJ
description The miracle fruit (Synsepalum dulcificum) is distinguished by its unique ability to alter taste perceptions, making it a low-calorie sugar substitute. This study aimed to determine its physicochemical characteristics and bioactive compounds by evaluating various extraction and preservation methods. Utilizing a Completely Randomized Block Design with a factorial arrangement (A∗B), where factor A represented extraction methods (cold pressing, maceration, and pressurized liquids) and factor B represented preservation methods (fluid extract and ionic emulsion), the research found that bromatological analyses showed pH values ranging from 2.990 to 3.535, acidity between 0.13 and 0.14, soluble solids from 4.45 to 41.00, and protein content from 0.029 to 4.670. The maceration with fluid extract method achieved the most favorable results in terms of bioactive compounds, including flavonoids (20.14 mg Eq/g), anthocyanins (9.55 mg/g), total phenolic content (16.53 mg gallic acid/g), and antioxidant capacity (521.24 μg Trolox/g). Conversely, cold pressing combined with fluid extract was the most effective for carotenoid extraction (77.16 μg/g). Thus, maceration with fluid extract is the most efficient for obtaining bioactive compounds, while cold pressing combined with fluid extract excels in carotenoid extraction.
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spelling doaj-art-713719c52957490f8450214e035c73ce2025-02-02T05:28:13ZengElsevierHeliyon2405-84402025-01-01112e41794Determination of the physicochemical characteristics and bioactive compounds of the miracle fruit (Synsepalum dulcificum) considering different extraction and preservation methodsJuan Alejandro Neira Mosquera0Sungey Naynee Sánchez Llaguno1Karol Yannela Revilla Escobar2Jhonnatan Placido Aldas Morejon3Angie Estefania Iguasnia Ureta4Andy Sebastian Parrales Loor5Jonathan Alexander Arguello Cedeño6Departamento de Ciencias de la Vida y Agricultura, Universidad de las Fuerzas Armadas-ESPE, Santo Domingo, EcuadorDepartamento de Ciencias de la Vida y Agricultura, Universidad de las Fuerzas Armadas-ESPE, Santo Domingo, EcuadorCarrera de agroindustria, Pontificia Universidad Católica del Ecuador SEDE Esmeraldas, Campus Tachina, Ecuador; Corresponding author.Facultad de Ciencias Aplicadas a la Industria, Universidad Nacional de Cuyo, M5600APG, San Rafael, ArgentinaDepartamento de Ciencias de la Vida y Agricultura, Universidad de las Fuerzas Armadas-ESPE, Santo Domingo, EcuadorDepartamento de Ciencias de la Vida y Agricultura, Universidad de las Fuerzas Armadas-ESPE, Santo Domingo, EcuadorCarrera de agroindustria, Pontificia Universidad Católica del Ecuador SEDE Esmeraldas, Campus Tachina, EcuadorThe miracle fruit (Synsepalum dulcificum) is distinguished by its unique ability to alter taste perceptions, making it a low-calorie sugar substitute. This study aimed to determine its physicochemical characteristics and bioactive compounds by evaluating various extraction and preservation methods. Utilizing a Completely Randomized Block Design with a factorial arrangement (A∗B), where factor A represented extraction methods (cold pressing, maceration, and pressurized liquids) and factor B represented preservation methods (fluid extract and ionic emulsion), the research found that bromatological analyses showed pH values ranging from 2.990 to 3.535, acidity between 0.13 and 0.14, soluble solids from 4.45 to 41.00, and protein content from 0.029 to 4.670. The maceration with fluid extract method achieved the most favorable results in terms of bioactive compounds, including flavonoids (20.14 mg Eq/g), anthocyanins (9.55 mg/g), total phenolic content (16.53 mg gallic acid/g), and antioxidant capacity (521.24 μg Trolox/g). Conversely, cold pressing combined with fluid extract was the most effective for carotenoid extraction (77.16 μg/g). Thus, maceration with fluid extract is the most efficient for obtaining bioactive compounds, while cold pressing combined with fluid extract excels in carotenoid extraction.http://www.sciencedirect.com/science/article/pii/S2405844025001744Bioactive compoundsExtractsMacerationPressurized liquids
spellingShingle Juan Alejandro Neira Mosquera
Sungey Naynee Sánchez Llaguno
Karol Yannela Revilla Escobar
Jhonnatan Placido Aldas Morejon
Angie Estefania Iguasnia Ureta
Andy Sebastian Parrales Loor
Jonathan Alexander Arguello Cedeño
Determination of the physicochemical characteristics and bioactive compounds of the miracle fruit (Synsepalum dulcificum) considering different extraction and preservation methods
Heliyon
Bioactive compounds
Extracts
Maceration
Pressurized liquids
title Determination of the physicochemical characteristics and bioactive compounds of the miracle fruit (Synsepalum dulcificum) considering different extraction and preservation methods
title_full Determination of the physicochemical characteristics and bioactive compounds of the miracle fruit (Synsepalum dulcificum) considering different extraction and preservation methods
title_fullStr Determination of the physicochemical characteristics and bioactive compounds of the miracle fruit (Synsepalum dulcificum) considering different extraction and preservation methods
title_full_unstemmed Determination of the physicochemical characteristics and bioactive compounds of the miracle fruit (Synsepalum dulcificum) considering different extraction and preservation methods
title_short Determination of the physicochemical characteristics and bioactive compounds of the miracle fruit (Synsepalum dulcificum) considering different extraction and preservation methods
title_sort determination of the physicochemical characteristics and bioactive compounds of the miracle fruit synsepalum dulcificum considering different extraction and preservation methods
topic Bioactive compounds
Extracts
Maceration
Pressurized liquids
url http://www.sciencedirect.com/science/article/pii/S2405844025001744
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