A toolkit for facilitating markerless integration of expression cassettes in Komagataella phaffii via CRISPR/Cas9

A toolkit for facilitating markerless integration of expression cassettes in Komagataella phaffii via CRISPR/Cas9

Abstract Background The yeast Komagataella phaffii (formerly known as Pichia pastoris) has been widely used for functional expression of recombinant proteins, including plant and animal food proteins. CRISPR/Cas9 genome editing systems can be used for insertion of heterologous genes without the use...

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Bibliographic Details
Main Authors: Laura García-Calvo, Charlotte Kummen, Solvor Rustad, Sissel Beate Rønning, Annette Fagerlund
Format: Article
Language:English
Published: BMC 2025-05-01
Series:Microbial Cell Factories
Subjects:
Pichia pastoris
Komagataella phaffii
CRISPR/Cas9
Heterologous expression
Golden Gate assembly
Food proteins
Online Access:https://doi.org/10.1186/s12934-025-02716-x
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https://doi.org/10.1186/s12934-025-02716-x

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