Prevalence, assessment, and antimicrobial resistance patterns of Salmonella from raw chicken eggs in Haramaya, Ethiopia

Introduction: The presence of antimicrobial-resistant Salmonella in poultry and poultry products, including eggs, is a global public health concern. This study aimed to estimate the levels and patterns of antimicrobial resistance of Salmonella from chicken eggs and assess consumers’ raw egg consumpt...

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Main Authors: Jelalu Kemal, Berhanu Sibhat, Sissay Menkir, Desta Beyene
Format: Article
Language:English
Published: The Journal of Infection in Developing Countries 2016-11-01
Series:Journal of Infection in Developing Countries
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Online Access:https://jidc.org/index.php/journal/article/view/7885
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author Jelalu Kemal
Berhanu Sibhat
Sissay Menkir
Desta Beyene
author_facet Jelalu Kemal
Berhanu Sibhat
Sissay Menkir
Desta Beyene
author_sort Jelalu Kemal
collection DOAJ
description Introduction: The presence of antimicrobial-resistant Salmonella in poultry and poultry products, including eggs, is a global public health concern. This study aimed to estimate the levels and patterns of antimicrobial resistance of Salmonella from chicken eggs and assess consumers’ raw egg consumption and farmers’ handling practices. Methodology: A total of 300 egg samples were collected from Haramaya open market (n = 150) and Haramaya University poultry farm (n = 150) in Ethiopia. Questionnaires were administered to egg sellers and buyers. A sterile cotton swab was used to sample the surface of eggs. The shells were sterilized and the egg content sampled. Isolation was done using the conventional methods for the detection of Salmonella, following the standard guidelines from ISO 6579. Sensitivity to 12 selected antibiotics was tested following the procedure of the Clinical and Laboratory Standards Institute. Results: A level of 5.3% was observed among eggs shells from the open market and 0% among egg shells from the poultry farm, for an overall level of 2.7%. There was a significant difference (p = 0.004) between the prevalence of Salmonella spp. in sample site and sample type. Of the antimicrobials tested, Salmonella isolates were all resistant to erythromycin and clindamycin. Isolates were sensitive to ciprofloxacin (100%) and chloramphenicol (87.5%). All isolates were resistant to multiple antibiotics. One-third of the consumers were found to have eaten raw eggs for perceived medicinal values. Conclusion: To minimize the potential contamination of eggs by pathogens, the eggs should be properly handled, transported, and stored.
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spelling doaj-art-70eae6590bad49179e3dd2e30a540fe72025-08-20T03:48:46ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802016-11-01101110.3855/jidc.7885Prevalence, assessment, and antimicrobial resistance patterns of Salmonella from raw chicken eggs in Haramaya, EthiopiaJelalu Kemal0Berhanu Sibhat1Sissay Menkir2Desta Beyene3Haramaya University, Dire Dawa, EthiopiaHaramaya University, Dire Dawa, EthiopiaHaramaya University, Dire Dawa, EthiopiaHaramaya University, Dire Dawa, EthiopiaIntroduction: The presence of antimicrobial-resistant Salmonella in poultry and poultry products, including eggs, is a global public health concern. This study aimed to estimate the levels and patterns of antimicrobial resistance of Salmonella from chicken eggs and assess consumers’ raw egg consumption and farmers’ handling practices. Methodology: A total of 300 egg samples were collected from Haramaya open market (n = 150) and Haramaya University poultry farm (n = 150) in Ethiopia. Questionnaires were administered to egg sellers and buyers. A sterile cotton swab was used to sample the surface of eggs. The shells were sterilized and the egg content sampled. Isolation was done using the conventional methods for the detection of Salmonella, following the standard guidelines from ISO 6579. Sensitivity to 12 selected antibiotics was tested following the procedure of the Clinical and Laboratory Standards Institute. Results: A level of 5.3% was observed among eggs shells from the open market and 0% among egg shells from the poultry farm, for an overall level of 2.7%. There was a significant difference (p = 0.004) between the prevalence of Salmonella spp. in sample site and sample type. Of the antimicrobials tested, Salmonella isolates were all resistant to erythromycin and clindamycin. Isolates were sensitive to ciprofloxacin (100%) and chloramphenicol (87.5%). All isolates were resistant to multiple antibiotics. One-third of the consumers were found to have eaten raw eggs for perceived medicinal values. Conclusion: To minimize the potential contamination of eggs by pathogens, the eggs should be properly handled, transported, and stored. https://jidc.org/index.php/journal/article/view/7885antimicrobial sensitivitychicken eggEthiopiaSalmonella
spellingShingle Jelalu Kemal
Berhanu Sibhat
Sissay Menkir
Desta Beyene
Prevalence, assessment, and antimicrobial resistance patterns of Salmonella from raw chicken eggs in Haramaya, Ethiopia
Journal of Infection in Developing Countries
antimicrobial sensitivity
chicken egg
Ethiopia
Salmonella
title Prevalence, assessment, and antimicrobial resistance patterns of Salmonella from raw chicken eggs in Haramaya, Ethiopia
title_full Prevalence, assessment, and antimicrobial resistance patterns of Salmonella from raw chicken eggs in Haramaya, Ethiopia
title_fullStr Prevalence, assessment, and antimicrobial resistance patterns of Salmonella from raw chicken eggs in Haramaya, Ethiopia
title_full_unstemmed Prevalence, assessment, and antimicrobial resistance patterns of Salmonella from raw chicken eggs in Haramaya, Ethiopia
title_short Prevalence, assessment, and antimicrobial resistance patterns of Salmonella from raw chicken eggs in Haramaya, Ethiopia
title_sort prevalence assessment and antimicrobial resistance patterns of salmonella from raw chicken eggs in haramaya ethiopia
topic antimicrobial sensitivity
chicken egg
Ethiopia
Salmonella
url https://jidc.org/index.php/journal/article/view/7885
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