Streamlining homogeneous glycoprotein production for biophysical and structural applications by targeted cell line development.

Streamlining homogeneous glycoprotein production for biophysical and structural applications by targeted cell line development.

Studying the biophysical characteristics of glycosylated proteins and solving their three-dimensional structures requires homogeneous recombinant protein of high quality.We introduce here a new approach to produce glycoproteins in homogenous form with the well-established, glycosylation mutant CHO L...

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Main Authors: Sonja Wilke, Lothar Groebe, Vitali Maffenbeier, Volker Jäger, Manfred Gossen, Jörn Josewski, Agathe Duda, Lilia Polle, Raymond J Owens, Dagmar Wirth, Dirk W Heinz, Joop van den Heuvel, Konrad Büssow
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0027829&type=printable
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https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0027829&type=printable

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