Identification of robust RT-qPCR reference genes for studying changes in gene expression in response to hypoxia in breast cancer cell lines

Abstract Hypoxia is common in breast tumours and is linked to therapy resistance and advanced disease. To understand hypoxia-driven breast cancer progression, RT-qPCR is a widely used technique to quantify transcriptional changes that occur during malignant transformation. Reference genes (RGs) are...

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Bibliographic Details
Main Authors: Jodie R. Malcolm, Katherine S. Bridge, Andrew N. Holding, William J. Brackenbury
Format: Article
Language:English
Published: BMC 2025-01-01
Series:BMC Genomics
Online Access:https://doi.org/10.1186/s12864-025-11216-6
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