Identification of genetic factors influencing flavonoid biosynthesis through pooled transcriptome analysis in mungbean sprouts
IntroductionMungbean (Vigna radiata L.) is gaining increasing interest among legume crops because of its nutritional value. Various secondary metabolites that act as antioxidants and bioactive compounds are beneficial for human health. The secondary metabolite content in plants is easily influenced...
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1540674/full |
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| author | Yeonghun Cho Hakyung Kwon Hakyung Kwon Byeong Cheol Kim Donghwan Shim Jungmin Ha Jungmin Ha |
| author_facet | Yeonghun Cho Hakyung Kwon Hakyung Kwon Byeong Cheol Kim Donghwan Shim Jungmin Ha Jungmin Ha |
| author_sort | Yeonghun Cho |
| collection | DOAJ |
| description | IntroductionMungbean (Vigna radiata L.) is gaining increasing interest among legume crops because of its nutritional value. Various secondary metabolites that act as antioxidants and bioactive compounds are beneficial for human health. The secondary metabolite content in plants is easily influenced by environmental conditions, and this influence varies depending on the genotype.Materials and MethodsHere, we screened six genotypes with consistently high and low content of major secondary metabolites (gallic acid, chlorogenic acid, neo-chlorogenic acid, genistin, formononetin, catechin, syringic acid, and resveratrol) across environmental replicates. Transcriptome data obtained from the individual genotypes were pooled into two groups: high and low levels of secondary metabolites.Results and DiscussionOf the 200 differentially expressed genes identified using stringent criteria, 23 were annotated in the secondary metabolite pathway. By combining the results of the secondary metabolite and transcriptome data, we identified six key genes encoding four enzymes (CCoAOMT1; Caffeoyl-CoA O-methyltransferase, CYP81E1; 4'-methoxyisoflavone 2'-hydroxylase, DFR; dihydroflavonol-4-reductase, and HCT; shikimate O-hydroxycinnamoyltransferase) that commonly influence the content of secondary metabolites (catechin, chlorogenic acid, formononetin, and genistin) in mungbeans. Through regulatory network analysis, NAC042 and MYB74 transcription factors were identified. These transcription factors regulate the expression of four key genes in mungbean, CCoAOMT1(Vradi02g00000724.1), CYP81E1(Vradi09g00002897.1), DFR(Vradi07g00001336.1), and HCT(Vradi07g00000614.1) leading to high flavonoid content.ConclusionThese results provide information on the common genetic factors involved in the production of secondary metabolites, which can improve the nutritional value of mungbeans and contribute to the development of elite mungbean cultivars. |
| format | Article |
| id | doaj-art-7051c8e257d043619181e4e2ce900455 |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-7051c8e257d043619181e4e2ce9004552025-08-20T02:04:28ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-03-011610.3389/fpls.2025.15406741540674Identification of genetic factors influencing flavonoid biosynthesis through pooled transcriptome analysis in mungbean sproutsYeonghun Cho0Hakyung Kwon1Hakyung Kwon2Byeong Cheol Kim3Donghwan Shim4Jungmin Ha5Jungmin Ha6Department of Agriculture, Forestry and Bioresources and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of KoreaDepartment of Agriculture, Forestry and Bioresources and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of KoreaCrop Genomics Lab, Plant Genomics and Breeding Institute, Seoul National University, Seoul, Republic of KoreaDepartment of Plant Science, Gangneung-Wonju National University, Gangneung, Republic of KoreaDepartment of Biological Sciences, Chungnam National University, Daejeon, Republic of KoreaDepartment of Agriculture, Forestry and Bioresources and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of KoreaCrop Genomics Lab, Plant Genomics and Breeding Institute, Seoul National University, Seoul, Republic of KoreaIntroductionMungbean (Vigna radiata L.) is gaining increasing interest among legume crops because of its nutritional value. Various secondary metabolites that act as antioxidants and bioactive compounds are beneficial for human health. The secondary metabolite content in plants is easily influenced by environmental conditions, and this influence varies depending on the genotype.Materials and MethodsHere, we screened six genotypes with consistently high and low content of major secondary metabolites (gallic acid, chlorogenic acid, neo-chlorogenic acid, genistin, formononetin, catechin, syringic acid, and resveratrol) across environmental replicates. Transcriptome data obtained from the individual genotypes were pooled into two groups: high and low levels of secondary metabolites.Results and DiscussionOf the 200 differentially expressed genes identified using stringent criteria, 23 were annotated in the secondary metabolite pathway. By combining the results of the secondary metabolite and transcriptome data, we identified six key genes encoding four enzymes (CCoAOMT1; Caffeoyl-CoA O-methyltransferase, CYP81E1; 4'-methoxyisoflavone 2'-hydroxylase, DFR; dihydroflavonol-4-reductase, and HCT; shikimate O-hydroxycinnamoyltransferase) that commonly influence the content of secondary metabolites (catechin, chlorogenic acid, formononetin, and genistin) in mungbeans. Through regulatory network analysis, NAC042 and MYB74 transcription factors were identified. These transcription factors regulate the expression of four key genes in mungbean, CCoAOMT1(Vradi02g00000724.1), CYP81E1(Vradi09g00002897.1), DFR(Vradi07g00001336.1), and HCT(Vradi07g00000614.1) leading to high flavonoid content.ConclusionThese results provide information on the common genetic factors involved in the production of secondary metabolites, which can improve the nutritional value of mungbeans and contribute to the development of elite mungbean cultivars.https://www.frontiersin.org/articles/10.3389/fpls.2025.1540674/fullmungbean sproutultra-high-performance liquid chromatographysecondary metabolite contentsample poolingRNA-Seqgene expression |
| spellingShingle | Yeonghun Cho Hakyung Kwon Hakyung Kwon Byeong Cheol Kim Donghwan Shim Jungmin Ha Jungmin Ha Identification of genetic factors influencing flavonoid biosynthesis through pooled transcriptome analysis in mungbean sprouts Frontiers in Plant Science mungbean sprout ultra-high-performance liquid chromatography secondary metabolite content sample pooling RNA-Seq gene expression |
| title | Identification of genetic factors influencing flavonoid biosynthesis through pooled transcriptome analysis in mungbean sprouts |
| title_full | Identification of genetic factors influencing flavonoid biosynthesis through pooled transcriptome analysis in mungbean sprouts |
| title_fullStr | Identification of genetic factors influencing flavonoid biosynthesis through pooled transcriptome analysis in mungbean sprouts |
| title_full_unstemmed | Identification of genetic factors influencing flavonoid biosynthesis through pooled transcriptome analysis in mungbean sprouts |
| title_short | Identification of genetic factors influencing flavonoid biosynthesis through pooled transcriptome analysis in mungbean sprouts |
| title_sort | identification of genetic factors influencing flavonoid biosynthesis through pooled transcriptome analysis in mungbean sprouts |
| topic | mungbean sprout ultra-high-performance liquid chromatography secondary metabolite content sample pooling RNA-Seq gene expression |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1540674/full |
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