Long Noncoding RNA–Messenger RNA (lncRNA–mRNA) Network in Resistance to Gummy Stem Blight (<i>Stagonosporopsis cucurbitacearum</i>) of Melon Plant Introduction (PI) 420145
Gummy stem blight (GSB), caused by <i>Stagonosporopsis cucurbitacearum</i> (<i>Didymella bryoniae</i>), poses a growing threat to greenhouse melon production. Despite this, the defense mechanism of melon against <i>S. cucurbitacearum</i> remains poorly understood....
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2025-01-01
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| author | Rui Zhang Jing Chen Yongbing Zhang Xiumei Lu Chunyan Cheng Ji Li Qunfeng Lou Yuhui Wang Chuntao Qian |
| author_facet | Rui Zhang Jing Chen Yongbing Zhang Xiumei Lu Chunyan Cheng Ji Li Qunfeng Lou Yuhui Wang Chuntao Qian |
| author_sort | Rui Zhang |
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| description | Gummy stem blight (GSB), caused by <i>Stagonosporopsis cucurbitacearum</i> (<i>Didymella bryoniae</i>), poses a growing threat to greenhouse melon production. Despite this, the defense mechanism of melon against <i>S. cucurbitacearum</i> remains poorly understood. In this study, by employing electron microscopy and transcriptome sequencing, we investigated the cellular ultrastructure differences and gene expression dynamics of two melon accessions, PI 420145 (resistant to GSB) and ‘Baipicui’ (susceptible), pre- and post- inoculation. Our results revealed that PI 420145 exhibits a thicker waxy layer on the leaf surface and limited conidial germination without obvious signs of cell damage compared to ‘Baipicui’. The transcriptomic analysis identified a total of 23,078 differentially expressed genes (DEGs) and 974 differentially expressed long noncoding RNAs (DELs). Specifically, phenylpropanoid biosynthesis, cyanoamino acid metabolism, and MAPK signaling–plant and plant–pathogen interactions were enriched in PI 420145, while ‘Baipicui’ displayed enrichment in metabolism and autophagy. Additionally, through lncRNA–mRNA network construction, we identified a total of 38 lncRNA–mRNA targeted regulatory relationships in the four most significant KEGG pathways for disease resistance. |
| format | Article |
| id | doaj-art-70490883e47643b0aa2e705d977f9a63 |
| institution | Kabale University |
| issn | 2311-7524 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Horticulturae |
| spelling | doaj-art-70490883e47643b0aa2e705d977f9a632025-01-24T13:34:31ZengMDPI AGHorticulturae2311-75242025-01-011113110.3390/horticulturae11010031Long Noncoding RNA–Messenger RNA (lncRNA–mRNA) Network in Resistance to Gummy Stem Blight (<i>Stagonosporopsis cucurbitacearum</i>) of Melon Plant Introduction (PI) 420145Rui Zhang0Jing Chen1Yongbing Zhang2Xiumei Lu3Chunyan Cheng4Ji Li5Qunfeng Lou6Yuhui Wang7Chuntao Qian8National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, No. 1 Weigang, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, No. 1 Weigang, Nanjing 210095, ChinaHami Melon Research Center, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, No. 1 Weigang, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, No. 1 Weigang, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, No. 1 Weigang, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, No. 1 Weigang, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, No. 1 Weigang, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, No. 1 Weigang, Nanjing 210095, ChinaGummy stem blight (GSB), caused by <i>Stagonosporopsis cucurbitacearum</i> (<i>Didymella bryoniae</i>), poses a growing threat to greenhouse melon production. Despite this, the defense mechanism of melon against <i>S. cucurbitacearum</i> remains poorly understood. In this study, by employing electron microscopy and transcriptome sequencing, we investigated the cellular ultrastructure differences and gene expression dynamics of two melon accessions, PI 420145 (resistant to GSB) and ‘Baipicui’ (susceptible), pre- and post- inoculation. Our results revealed that PI 420145 exhibits a thicker waxy layer on the leaf surface and limited conidial germination without obvious signs of cell damage compared to ‘Baipicui’. The transcriptomic analysis identified a total of 23,078 differentially expressed genes (DEGs) and 974 differentially expressed long noncoding RNAs (DELs). Specifically, phenylpropanoid biosynthesis, cyanoamino acid metabolism, and MAPK signaling–plant and plant–pathogen interactions were enriched in PI 420145, while ‘Baipicui’ displayed enrichment in metabolism and autophagy. Additionally, through lncRNA–mRNA network construction, we identified a total of 38 lncRNA–mRNA targeted regulatory relationships in the four most significant KEGG pathways for disease resistance.https://www.mdpi.com/2311-7524/11/1/31melongummy stem blightmicroscopic structuremRNAlncRNA |
| spellingShingle | Rui Zhang Jing Chen Yongbing Zhang Xiumei Lu Chunyan Cheng Ji Li Qunfeng Lou Yuhui Wang Chuntao Qian Long Noncoding RNA–Messenger RNA (lncRNA–mRNA) Network in Resistance to Gummy Stem Blight (<i>Stagonosporopsis cucurbitacearum</i>) of Melon Plant Introduction (PI) 420145 Horticulturae melon gummy stem blight microscopic structure mRNA lncRNA |
| title | Long Noncoding RNA–Messenger RNA (lncRNA–mRNA) Network in Resistance to Gummy Stem Blight (<i>Stagonosporopsis cucurbitacearum</i>) of Melon Plant Introduction (PI) 420145 |
| title_full | Long Noncoding RNA–Messenger RNA (lncRNA–mRNA) Network in Resistance to Gummy Stem Blight (<i>Stagonosporopsis cucurbitacearum</i>) of Melon Plant Introduction (PI) 420145 |
| title_fullStr | Long Noncoding RNA–Messenger RNA (lncRNA–mRNA) Network in Resistance to Gummy Stem Blight (<i>Stagonosporopsis cucurbitacearum</i>) of Melon Plant Introduction (PI) 420145 |
| title_full_unstemmed | Long Noncoding RNA–Messenger RNA (lncRNA–mRNA) Network in Resistance to Gummy Stem Blight (<i>Stagonosporopsis cucurbitacearum</i>) of Melon Plant Introduction (PI) 420145 |
| title_short | Long Noncoding RNA–Messenger RNA (lncRNA–mRNA) Network in Resistance to Gummy Stem Blight (<i>Stagonosporopsis cucurbitacearum</i>) of Melon Plant Introduction (PI) 420145 |
| title_sort | long noncoding rna messenger rna lncrna mrna network in resistance to gummy stem blight i stagonosporopsis cucurbitacearum i of melon plant introduction pi 420145 |
| topic | melon gummy stem blight microscopic structure mRNA lncRNA |
| url | https://www.mdpi.com/2311-7524/11/1/31 |
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