The Polymorphism of Voltage-Gated Sodium Channel Gene in Culex Mosquitoes in Banyuwangi District, Indonesia
Culex mosquito is able to act as transmission vector of filariasis and Japanese encephalitis. Both diseases occur in Indonesia and filariasis exists in Banyuwangi district. The Voltage-Gated Sodium Channel (VGSC) gene mutations are related to pyrethroid insecticide resistance, especially L1014F/S mu...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas
2024-11-01
|
| Series: | Andalasian International Journal of Entomology |
| Subjects: | |
| Online Access: | https://aijent.lppm.unand.ac.id/index.php/aijent/article/view/32 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832575457779777536 |
|---|---|
| author | Yasmin Azzahra Arifin Hanifah Dzakiyah Catherine Canina Charunita Sakura Alya Ashari Winna Putri Veliarta Muhammad Akbar Panjinegara Francesca Giovanetti Carlo Montagna Wojciech Jankowski Muhammad Fathoni Binarri Augustya Lynda Rossyanti Suhintam Pusarawati Sukmawati Basuki |
| author_facet | Yasmin Azzahra Arifin Hanifah Dzakiyah Catherine Canina Charunita Sakura Alya Ashari Winna Putri Veliarta Muhammad Akbar Panjinegara Francesca Giovanetti Carlo Montagna Wojciech Jankowski Muhammad Fathoni Binarri Augustya Lynda Rossyanti Suhintam Pusarawati Sukmawati Basuki |
| author_sort | Yasmin Azzahra Arifin |
| collection | DOAJ |
| description | Culex mosquito is able to act as transmission vector of filariasis and Japanese encephalitis. Both diseases occur in Indonesia and filariasis exists in Banyuwangi district. The Voltage-Gated Sodium Channel (VGSC) gene mutations are related to pyrethroid insecticide resistance, especially L1014F/S mutations have not yet been fully studied in Indonesia. This study aimed to investigate the polymorphism of VGSC gene mutation in Culex mosquitoes in Banyuwangi District of East Java Province, Indonesia. Culex mosquitoes were collected using mosquito trap in a two-day collection from Banyuwangi Sub-district and two sub-villages in Cluring Sub-district of Banyuwangi District in August 2023. Culex mosquitoes were identified by morphological examination and Culex DNA was extracted by Chelex100 from each Culex mosquito. The polymorphism of VGSC gene mutations of L1014F/S were determined by Allele Specific-Polymerase Chain Reaction (AS-PCR). A total of 62 Culex mosquitoes were analyzed. The wild type Culex mosquitoes were found predominant (77%, 48/62). Two types of homozygous mutations were identified as 1014F and 1014 S (16%, 10/62 and 2%, 1/62, respectively). The heterozygous mutation was only 1014F/S type with frequency of 5% (3/62). The predominance of wild type Culex mosquitoes indicated that Culex population in the study areas is still susceptible to pyrethroid insecticide. However, the mutant types of Culex are already occurred, so insecticide resistance among Culex mosquitoes is required to be regular monitoring. |
| format | Article |
| id | doaj-art-7025ecc3c3a74f899ff5a80b36501f17 |
| institution | Kabale University |
| issn | 3026-2461 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas |
| record_format | Article |
| series | Andalasian International Journal of Entomology |
| spelling | doaj-art-7025ecc3c3a74f899ff5a80b36501f172025-02-01T07:28:33ZengLembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas AndalasAndalasian International Journal of Entomology3026-24612024-11-012210611310.25077/aijent.2.2.106-113.202432The Polymorphism of Voltage-Gated Sodium Channel Gene in Culex Mosquitoes in Banyuwangi District, IndonesiaYasmin Azzahra Arifin0Hanifah Dzakiyah1Catherine Canina Charunita2Sakura Alya Ashari3Winna Putri Veliarta4Muhammad Akbar Panjinegara5Francesca Giovanetti6Carlo Montagna7Wojciech Jankowski8Muhammad Fathoni9Binarri Augustya10Lynda Rossyanti11Suhintam Pusarawati12Sukmawati Basuki13Bachelor Program of Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, IndonesiaBachelor Program of Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, IndonesiaBachelor Program of Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, IndonesiaBachelor Program of Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, IndonesiaBachelor Program of Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, IndonesiaBachelor Program of Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, IndonesiaUniversity of Padua, Milano, ItalyHumanitas University, Trento, ItalyUniwersytet Medyczny w Łodzi, Łódź, PolandPrimary Health Center, Benculuk, Cluring, Banyuwangi, IndonesiaDepartment of Medical Parasitology, Faculty of Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Medical Parasitology, Faculty of Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Medical Parasitology, Faculty of Medicine, Universitas Airlangga, Surabaya, IndonesiaMalaria Study Group/Laboratory of Malaria, Institute of Tropical Disease, Universitas Airlangga, Surabaya, IndonesiaCulex mosquito is able to act as transmission vector of filariasis and Japanese encephalitis. Both diseases occur in Indonesia and filariasis exists in Banyuwangi district. The Voltage-Gated Sodium Channel (VGSC) gene mutations are related to pyrethroid insecticide resistance, especially L1014F/S mutations have not yet been fully studied in Indonesia. This study aimed to investigate the polymorphism of VGSC gene mutation in Culex mosquitoes in Banyuwangi District of East Java Province, Indonesia. Culex mosquitoes were collected using mosquito trap in a two-day collection from Banyuwangi Sub-district and two sub-villages in Cluring Sub-district of Banyuwangi District in August 2023. Culex mosquitoes were identified by morphological examination and Culex DNA was extracted by Chelex100 from each Culex mosquito. The polymorphism of VGSC gene mutations of L1014F/S were determined by Allele Specific-Polymerase Chain Reaction (AS-PCR). A total of 62 Culex mosquitoes were analyzed. The wild type Culex mosquitoes were found predominant (77%, 48/62). Two types of homozygous mutations were identified as 1014F and 1014 S (16%, 10/62 and 2%, 1/62, respectively). The heterozygous mutation was only 1014F/S type with frequency of 5% (3/62). The predominance of wild type Culex mosquitoes indicated that Culex population in the study areas is still susceptible to pyrethroid insecticide. However, the mutant types of Culex are already occurred, so insecticide resistance among Culex mosquitoes is required to be regular monitoring.https://aijent.lppm.unand.ac.id/index.php/aijent/article/view/32culexvoltage-gated sodium channel genepolymorphismbanyuwangi districtindonesia |
| spellingShingle | Yasmin Azzahra Arifin Hanifah Dzakiyah Catherine Canina Charunita Sakura Alya Ashari Winna Putri Veliarta Muhammad Akbar Panjinegara Francesca Giovanetti Carlo Montagna Wojciech Jankowski Muhammad Fathoni Binarri Augustya Lynda Rossyanti Suhintam Pusarawati Sukmawati Basuki The Polymorphism of Voltage-Gated Sodium Channel Gene in Culex Mosquitoes in Banyuwangi District, Indonesia Andalasian International Journal of Entomology culex voltage-gated sodium channel gene polymorphism banyuwangi district indonesia |
| title | The Polymorphism of Voltage-Gated Sodium Channel Gene in Culex Mosquitoes in Banyuwangi District, Indonesia |
| title_full | The Polymorphism of Voltage-Gated Sodium Channel Gene in Culex Mosquitoes in Banyuwangi District, Indonesia |
| title_fullStr | The Polymorphism of Voltage-Gated Sodium Channel Gene in Culex Mosquitoes in Banyuwangi District, Indonesia |
| title_full_unstemmed | The Polymorphism of Voltage-Gated Sodium Channel Gene in Culex Mosquitoes in Banyuwangi District, Indonesia |
| title_short | The Polymorphism of Voltage-Gated Sodium Channel Gene in Culex Mosquitoes in Banyuwangi District, Indonesia |
| title_sort | polymorphism of voltage gated sodium channel gene in culex mosquitoes in banyuwangi district indonesia |
| topic | culex voltage-gated sodium channel gene polymorphism banyuwangi district indonesia |
| url | https://aijent.lppm.unand.ac.id/index.php/aijent/article/view/32 |
| work_keys_str_mv | AT yasminazzahraarifin thepolymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT hanifahdzakiyah thepolymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT catherinecaninacharunita thepolymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT sakuraalyaashari thepolymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT winnaputriveliarta thepolymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT muhammadakbarpanjinegara thepolymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT francescagiovanetti thepolymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT carlomontagna thepolymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT wojciechjankowski thepolymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT muhammadfathoni thepolymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT binarriaugustya thepolymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT lyndarossyanti thepolymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT suhintampusarawati thepolymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT sukmawatibasuki thepolymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT yasminazzahraarifin polymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT hanifahdzakiyah polymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT catherinecaninacharunita polymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT sakuraalyaashari polymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT winnaputriveliarta polymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT muhammadakbarpanjinegara polymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT francescagiovanetti polymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT carlomontagna polymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT wojciechjankowski polymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT muhammadfathoni polymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT binarriaugustya polymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT lyndarossyanti polymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT suhintampusarawati polymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia AT sukmawatibasuki polymorphismofvoltagegatedsodiumchannelgeneinculexmosquitoesinbanyuwangidistrictindonesia |