Effects of high temperature stress on antioxidative and non-specific immunity indices of one-year-old Alosa sapidissima
In order to investigate the adaptability of Alosa sapidissima to high temperature stress, they were placed under three water temperature gradients (24 ℃, 28 ℃, 30 ℃) for 96 h, and the changes of antioxidant enzyme as well as non-specific immune enzyme activities in their liver and serum were studied...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2021-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2019.12.091 |
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| Summary: | In order to investigate the adaptability of Alosa sapidissima to high temperature stress, they were placed under three water temperature gradients (24 ℃, 28 ℃, 30 ℃) for 96 h, and the changes of antioxidant enzyme as well as non-specific immune enzyme activities in their liver and serum were studied at different time (0, 3, 6, 12, 24, 48, 96 h) under different water temperatures. The results showed that in the liver, the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in the 28 ℃ and 30 ℃ groups increased over time, and the malondialdehyde (MDA) content increased and then decreased with time, while there were no significant changes in the 24 ℃ group; the activities of SOD and CAT at 48 and 96 h were significantly higher than those in the 24 ℃ group (P＜0.05), and the GSH-Px activity and MDA content in the 30 ℃ group at 96 h were significantly higher than those in the other two groups (P＜0.05). In the serum, SOD activity and MDA content in the 30 ℃ group showed changes that increased first, then decreased, and then increased with time. The SOD activity and MDA content in the 30 ℃ group at 96 h were significantly higher than those in the 24 ℃ and 28 ℃ groups (P＜0.05). The 24 ℃ and 28 ℃ groups remained basically stable. The CAT and GSH-Px activities in the 28 ℃ group at 48 h showed an increasing trend with time, and were significantly greater than the other two groups (P＜0.05), while the CAT and GSH-Px activities in the 30 ℃ group showed a decreasing trend with time, and at 24-96 h, it was significantly smaller than the other two groups (P＜0.05), and the 24 ℃ group remained basically stable. Under the high temperature stress, the alkaline phosphatase (AKP) and acid phosphatase (ACP) activities of liver in the 28 ℃ and 30 ℃ groups decreased with time (P＜0.05), while it was basically remained stable in the 24 ℃ group; the AKP and ACP activities at 48 and 96 h were significantly smaller than those in the 24 ℃ group (P＜0.05). The activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the 30 ℃ group increased with time, and the AST activity at 96 h and the ALT activity at 48 h were significantly higher than those in the 24 ℃ group (P＜0.05); the AST activity in the 28 ℃ group increased with time, and was significantly higher at 96 h than that in the 24 ℃ group (P＜0.05), while the ALT activity increased first and then decreased with time. The above results show that high temperature stress has a significant impact on the antioxidant and non-specific immune enzyme activities of A. sapidissima, causing certain damage to its liver. Therefore, in the process of practical production and industrial aquaculture, it is necessary to avoid high temperature stress response from A. sapidissima, and recommend that the breeding temperature is controlled below 28 ℃. |
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| ISSN: | 1008-9209 2097-5155 |