Protocol for generating human craniofacial cartilage organoids from stem-cell-derived neural crest cells

Summary: Here, we present a protocol to generate craniofacial cartilage organoids from human stem cells via neural crest stem cells (NCSCs). We describe steps for inducing human embryonic stem cells (hESCs) or induced pluripotent stem cells (iPSCs) to form NCSCs using sequential treatments of small...

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Bibliographic Details
Main Authors: Nagashree Avabhrath, Lauren Foltz, Mark Grimes
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S266616672400697X
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