Impact of Fermented Soy Beverages Containing Selected Vaginal Probiotics on the In Vitro Fecal Microbiota of Post-Menopausal Women
The gut microbiome of women can change after menopause, and during this phase women can also be more susceptible to vaginal dysbiosis. Recent studies have explored the probiotic potential of <i>Lactobacillus crispatus</i> BC4 and <i>Lactobacillus gasseri</i> BC9 against vario...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/14/6/1022 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850204420637196288 |
|---|---|
| author | Margherita D’Alessandro Davide Gottardi Silvia Arboleya Guadalupe Monserrat Alvarado-Jasso Carola Parolin Beatrice Vitali Rosalba Lanciotti Miguel Gueimonde Francesca Patrignani |
| author_facet | Margherita D’Alessandro Davide Gottardi Silvia Arboleya Guadalupe Monserrat Alvarado-Jasso Carola Parolin Beatrice Vitali Rosalba Lanciotti Miguel Gueimonde Francesca Patrignani |
| author_sort | Margherita D’Alessandro |
| collection | DOAJ |
| description | The gut microbiome of women can change after menopause, and during this phase women can also be more susceptible to vaginal dysbiosis. Recent studies have explored the probiotic potential of <i>Lactobacillus crispatus</i> BC4 and <i>Lactobacillus gasseri</i> BC9 against various pathogens and their use as co-starters in foods. However, their effects on the gut microbiota of post-menopausal women, who are more prone to dysbiosis, have not been examined. This study investigated the effects of predigested soy beverages (INFOGEST) containing BC4 and BC9 (encapsulated or not) on the composition and metabolic activity of the gut microbiota in post-menopausal women, using a fecal batch culture model. Parameters such as pH, gas, SCFAs, and microbiota composition (targeted qPCR and 16S rRNA gene sequencing) were assessed. The study, while highlighting a strong variability among donors, showed differences in gut microbiota response to the tested products. For instance, donor 2 showed a significant increase in bifidobacteria with BC4 + BC9 and E-BC9, while BC4 increased <i>Ruminococcaceae</i> in donors 1 and 3, and E-BC4 and E-BC9 enhanced <i>Akkermansia</i> in donor 1. BC4, E-BC4, E-BC9, and E-BC4 + BC9 significantly impacted metabolic activity, as measured by SCFAs, compared to other samples. However, no significant differences in gas production were observed. |
| format | Article |
| id | doaj-art-6f0e737bdd2a41fa877ae5afb29a722d |
| institution | OA Journals |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-6f0e737bdd2a41fa877ae5afb29a722d2025-08-20T02:11:17ZengMDPI AGFoods2304-81582025-03-01146102210.3390/foods14061022Impact of Fermented Soy Beverages Containing Selected Vaginal Probiotics on the In Vitro Fecal Microbiota of Post-Menopausal WomenMargherita D’Alessandro0Davide Gottardi1Silvia Arboleya2Guadalupe Monserrat Alvarado-Jasso3Carola Parolin4Beatrice Vitali5Rosalba Lanciotti6Miguel Gueimonde7Francesca Patrignani8Interdepartmental Center for Industrial Agri-Food Research, University of Bologna, Via Quinto Bucci 336, 47521 Cesena, ItalyInterdepartmental Center for Industrial Agri-Food Research, University of Bologna, Via Quinto Bucci 336, 47521 Cesena, ItalyDepartment of Microbiology and Biochemistry of Dairy Products, Dairy Research Institute IPLA-CSIC, C/Francisco Pintado Fe 26, 33011 Oviedo, SpainDepartment of Microbiology and Biochemistry of Dairy Products, Dairy Research Institute IPLA-CSIC, C/Francisco Pintado Fe 26, 33011 Oviedo, SpainDepartment of Pharmacy and Biotechnology, University of Bologna, Via San Donato 19/2, 40127 Bologna, ItalyDepartment of Pharmacy and Biotechnology, University of Bologna, Via San Donato 19/2, 40127 Bologna, ItalyInterdepartmental Center for Industrial Agri-Food Research, University of Bologna, Via Quinto Bucci 336, 47521 Cesena, ItalyDepartment of Microbiology and Biochemistry of Dairy Products, Dairy Research Institute IPLA-CSIC, C/Francisco Pintado Fe 26, 33011 Oviedo, SpainInterdepartmental Center for Industrial Agri-Food Research, University of Bologna, Via Quinto Bucci 336, 47521 Cesena, ItalyThe gut microbiome of women can change after menopause, and during this phase women can also be more susceptible to vaginal dysbiosis. Recent studies have explored the probiotic potential of <i>Lactobacillus crispatus</i> BC4 and <i>Lactobacillus gasseri</i> BC9 against various pathogens and their use as co-starters in foods. However, their effects on the gut microbiota of post-menopausal women, who are more prone to dysbiosis, have not been examined. This study investigated the effects of predigested soy beverages (INFOGEST) containing BC4 and BC9 (encapsulated or not) on the composition and metabolic activity of the gut microbiota in post-menopausal women, using a fecal batch culture model. Parameters such as pH, gas, SCFAs, and microbiota composition (targeted qPCR and 16S rRNA gene sequencing) were assessed. The study, while highlighting a strong variability among donors, showed differences in gut microbiota response to the tested products. For instance, donor 2 showed a significant increase in bifidobacteria with BC4 + BC9 and E-BC9, while BC4 increased <i>Ruminococcaceae</i> in donors 1 and 3, and E-BC4 and E-BC9 enhanced <i>Akkermansia</i> in donor 1. BC4, E-BC4, E-BC9, and E-BC4 + BC9 significantly impacted metabolic activity, as measured by SCFAs, compared to other samples. However, no significant differences in gas production were observed.https://www.mdpi.com/2304-8158/14/6/1022probioticsvaginal lactobacillisoy beveragesgut microbiotapost-menopausal womenmicrobial dysbiosis |
| spellingShingle | Margherita D’Alessandro Davide Gottardi Silvia Arboleya Guadalupe Monserrat Alvarado-Jasso Carola Parolin Beatrice Vitali Rosalba Lanciotti Miguel Gueimonde Francesca Patrignani Impact of Fermented Soy Beverages Containing Selected Vaginal Probiotics on the In Vitro Fecal Microbiota of Post-Menopausal Women Foods probiotics vaginal lactobacilli soy beverages gut microbiota post-menopausal women microbial dysbiosis |
| title | Impact of Fermented Soy Beverages Containing Selected Vaginal Probiotics on the In Vitro Fecal Microbiota of Post-Menopausal Women |
| title_full | Impact of Fermented Soy Beverages Containing Selected Vaginal Probiotics on the In Vitro Fecal Microbiota of Post-Menopausal Women |
| title_fullStr | Impact of Fermented Soy Beverages Containing Selected Vaginal Probiotics on the In Vitro Fecal Microbiota of Post-Menopausal Women |
| title_full_unstemmed | Impact of Fermented Soy Beverages Containing Selected Vaginal Probiotics on the In Vitro Fecal Microbiota of Post-Menopausal Women |
| title_short | Impact of Fermented Soy Beverages Containing Selected Vaginal Probiotics on the In Vitro Fecal Microbiota of Post-Menopausal Women |
| title_sort | impact of fermented soy beverages containing selected vaginal probiotics on the in vitro fecal microbiota of post menopausal women |
| topic | probiotics vaginal lactobacilli soy beverages gut microbiota post-menopausal women microbial dysbiosis |
| url | https://www.mdpi.com/2304-8158/14/6/1022 |
| work_keys_str_mv | AT margheritadalessandro impactoffermentedsoybeveragescontainingselectedvaginalprobioticsontheinvitrofecalmicrobiotaofpostmenopausalwomen AT davidegottardi impactoffermentedsoybeveragescontainingselectedvaginalprobioticsontheinvitrofecalmicrobiotaofpostmenopausalwomen AT silviaarboleya impactoffermentedsoybeveragescontainingselectedvaginalprobioticsontheinvitrofecalmicrobiotaofpostmenopausalwomen AT guadalupemonserratalvaradojasso impactoffermentedsoybeveragescontainingselectedvaginalprobioticsontheinvitrofecalmicrobiotaofpostmenopausalwomen AT carolaparolin impactoffermentedsoybeveragescontainingselectedvaginalprobioticsontheinvitrofecalmicrobiotaofpostmenopausalwomen AT beatricevitali impactoffermentedsoybeveragescontainingselectedvaginalprobioticsontheinvitrofecalmicrobiotaofpostmenopausalwomen AT rosalbalanciotti impactoffermentedsoybeveragescontainingselectedvaginalprobioticsontheinvitrofecalmicrobiotaofpostmenopausalwomen AT miguelgueimonde impactoffermentedsoybeveragescontainingselectedvaginalprobioticsontheinvitrofecalmicrobiotaofpostmenopausalwomen AT francescapatrignani impactoffermentedsoybeveragescontainingselectedvaginalprobioticsontheinvitrofecalmicrobiotaofpostmenopausalwomen |