Unfolded protein response during the progression of colorectal carcinogenesis
ABSTRACT Purpose: To evaluate the molecular evolution of endoplasmic reticulum (ER) stress during colorectal cancer carcinogenesis. Methods: Fifty-six hairless mice were divided into two groups: control (no intervention); and carcinogenesis (treated with two doses of azoxymethane at 10 mg/kg durin...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2025-01-01
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| Series: | Acta Cirúrgica Brasileira |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100204&lng=en&tlng=en |
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| _version_ | 1841538723536175104 |
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| author | Cesar Augusto Sobrinho Alexandre Moreira de Almeida Maytana Roberta Taschin Grigolo Michele Selzler Antônio Carlos de Abreu Letícia Silva Fagundes Almir Souza Martins Durval Batista Palhares Rondon Tosta Ramalho |
| author_facet | Cesar Augusto Sobrinho Alexandre Moreira de Almeida Maytana Roberta Taschin Grigolo Michele Selzler Antônio Carlos de Abreu Letícia Silva Fagundes Almir Souza Martins Durval Batista Palhares Rondon Tosta Ramalho |
| author_sort | Cesar Augusto Sobrinho |
| collection | DOAJ |
| description | ABSTRACT Purpose: To evaluate the molecular evolution of endoplasmic reticulum (ER) stress during colorectal cancer carcinogenesis. Methods: Fifty-six hairless mice were divided into two groups: control (no intervention); and carcinogenesis (treated with two doses of azoxymethane at 10 mg/kg during the third and the fourth week and dextran sodium sulfate at 2.5% for seven days in the second, fifth, and eighth week). Euthanasia occurred at the fifth, 10th, 15th, and 20th week. Colons were collected, and gene expression of ER stress markers (IRE1-α, PERK, ATF6, and CHOP) was assessed via real-time quantitative polymerase chain reaction. Results: ERN1 expression was significantly higher than the control at the 10th week (1.39 ± 0.16, p 0.05) and the 20th week (1.15 ± 0.04, p 0.01). ATF6 also showed elevated expression, significantly different at the 10th week (1.71 ± 0.29, p 0.05) and the 20th week (1.14 ± 0.06, p 0.05). PERK and CHOP expressions were significantly higher than the control in the 15th (PERK = 1.30 ± 0.12, CHOP = 1.48 ± 0.23) and 20th weeks (PERK = 1.63 ± 0.20, CHOP = 1.67 ± 0.22, p 0.05). Conclusion: Upregulation of IRN1, PERK, ATF6, and CHOP demonstrates a strong ER stress response during colorectal cancer development. |
| format | Article |
| id | doaj-art-6e51e46fb4dd4ac4b806c7b879124840 |
| institution | Kabale University |
| issn | 1678-2674 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj-art-6e51e46fb4dd4ac4b806c7b8791248402025-01-14T07:39:51ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira1678-26742025-01-014010.1590/acb400725Unfolded protein response during the progression of colorectal carcinogenesisCesar Augusto Sobrinhohttps://orcid.org/0000-0002-4277-9775Alexandre Moreira de Almeidahttps://orcid.org/0000-0001-8832-0284Maytana Roberta Taschin Grigolohttps://orcid.org/0009-0000-8267-1139Michele Selzlerhttps://orcid.org/0000-0001-7509-8688Antônio Carlos de Abreuhttps://orcid.org/0000-0001-5052-7028Letícia Silva Fagundeshttps://orcid.org/0000-0002-7693-9343Almir Souza Martinshttps://orcid.org/0000-0002-4379-685XDurval Batista Palhareshttps://orcid.org/0000-0002-4300-6125Rondon Tosta Ramalhohttps://orcid.org/0000-0002-6193-7521ABSTRACT Purpose: To evaluate the molecular evolution of endoplasmic reticulum (ER) stress during colorectal cancer carcinogenesis. Methods: Fifty-six hairless mice were divided into two groups: control (no intervention); and carcinogenesis (treated with two doses of azoxymethane at 10 mg/kg during the third and the fourth week and dextran sodium sulfate at 2.5% for seven days in the second, fifth, and eighth week). Euthanasia occurred at the fifth, 10th, 15th, and 20th week. Colons were collected, and gene expression of ER stress markers (IRE1-α, PERK, ATF6, and CHOP) was assessed via real-time quantitative polymerase chain reaction. Results: ERN1 expression was significantly higher than the control at the 10th week (1.39 ± 0.16, p 0.05) and the 20th week (1.15 ± 0.04, p 0.01). ATF6 also showed elevated expression, significantly different at the 10th week (1.71 ± 0.29, p 0.05) and the 20th week (1.14 ± 0.06, p 0.05). PERK and CHOP expressions were significantly higher than the control in the 15th (PERK = 1.30 ± 0.12, CHOP = 1.48 ± 0.23) and 20th weeks (PERK = 1.63 ± 0.20, CHOP = 1.67 ± 0.22, p 0.05). Conclusion: Upregulation of IRN1, PERK, ATF6, and CHOP demonstrates a strong ER stress response during colorectal cancer development.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100204&lng=en&tlng=enColorectal NeoplasmsEndoplasmic Reticulum StressUnfolded Protein Response |
| spellingShingle | Cesar Augusto Sobrinho Alexandre Moreira de Almeida Maytana Roberta Taschin Grigolo Michele Selzler Antônio Carlos de Abreu Letícia Silva Fagundes Almir Souza Martins Durval Batista Palhares Rondon Tosta Ramalho Unfolded protein response during the progression of colorectal carcinogenesis Acta Cirúrgica Brasileira Colorectal Neoplasms Endoplasmic Reticulum Stress Unfolded Protein Response |
| title | Unfolded protein response during the progression of colorectal carcinogenesis |
| title_full | Unfolded protein response during the progression of colorectal carcinogenesis |
| title_fullStr | Unfolded protein response during the progression of colorectal carcinogenesis |
| title_full_unstemmed | Unfolded protein response during the progression of colorectal carcinogenesis |
| title_short | Unfolded protein response during the progression of colorectal carcinogenesis |
| title_sort | unfolded protein response during the progression of colorectal carcinogenesis |
| topic | Colorectal Neoplasms Endoplasmic Reticulum Stress Unfolded Protein Response |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100204&lng=en&tlng=en |
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