CDK-mediated phosphorylation of PNKP is required for end-processing of single-strand DNA gaps on Okazaki fragments and genome stability

CDK-mediated phosphorylation of PNKP is required for end-processing of single-strand DNA gaps on Okazaki fragments and genome stability

Polynucleotide kinase phosphatase (PNKP) has enzymatic activities as 3′-phosphatase and 5′-kinase of DNA ends to promote DNA ligation and repair. Here, we show that cyclin-dependent kinases (CDKs) regulate the phosphorylation of threonine 118 (T118) in PNKP. This phosphorylation allows recruitment t...

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Bibliographic Details
Main Authors: Kaima Tsukada, Rikiya Imamura, Tomoko Miyake, Kotaro Saikawa, Mizuki Saito, Naoya Kase, Lingyan Fu, Masamichi Ishiai, Yoshihisa Matsumoto, Mikio Shimada
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2025-03-01
Series:eLife
Subjects:
PNKP
DNA replication
Okazaki fragment
DNA repair
Online Access:https://elifesciences.org/articles/99217
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https://elifesciences.org/articles/99217

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