Near-Infrared Spectroscopy and Chemometrics for the Routine Detection of Bilberry Extract Adulteration and Quantitative Determination of the Anthocyanins
Consumers must be assured that bought food supplements contain both bilberry extract and the anthocyanin amounts that match the declared levels. Therefore, a Fourier transform near-infrared (FT-NIR) spectroscopic method was validated based on principal component scores for the prediction of bilberry...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2018/4751247 |
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| author | Claudio Gardana Antonio Scialpi Christian Fachechi Paolo Simonetti |
| author_facet | Claudio Gardana Antonio Scialpi Christian Fachechi Paolo Simonetti |
| author_sort | Claudio Gardana |
| collection | DOAJ |
| description | Consumers must be assured that bought food supplements contain both bilberry extract and the anthocyanin amounts that match the declared levels. Therefore, a Fourier transform near-infrared (FT-NIR) spectroscopic method was validated based on principal component scores for the prediction of bilberry extract adulteration and partial least squares regression model for total anthocyanin evaluation. Anthocyanins have been quantified individually in 71 commercial bilberry extracts by HPLC-DAD, and 6 of them were counterfeit. The anthocyanin content in bilberry extracts was in the range 18–34%. Authentic bilberry extracts (n=65) were divided into two parts: one for calibration (n=38) and the other for the validation set (n=27). Spectra were recorded in the range of 4000–12500 cm−1, and a good prediction model was obtained in the range of 9400–6096 and 5456–4248 cm−1 with r2 of 99.5% and a root-mean-square error of 0.3%. The adulterated extracts subjected to NIR analysis were recognized as noncompliant, thus confirming the results obtained by chromatography. The FT-NIR spectroscopy is an economic, powerful, and fast methodology for the detection of adulteration and quantification of the total anthocyanin in bilberry extracts; above all, it is a rapid, low cost, and nondestructive technique for routine analysis. |
| format | Article |
| id | doaj-art-6d85a886b276442a83b127b15ba8ce1c |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-6d85a886b276442a83b127b15ba8ce1c2025-02-03T05:43:50ZengWileyJournal of Spectroscopy2314-49202314-49392018-01-01201810.1155/2018/47512474751247Near-Infrared Spectroscopy and Chemometrics for the Routine Detection of Bilberry Extract Adulteration and Quantitative Determination of the AnthocyaninsClaudio Gardana0Antonio Scialpi1Christian Fachechi2Paolo Simonetti3Department of Food, Environmental and Nutritional Sciences (DeFENS), Università Degli Studi di Milano, Via Celoria 2, 20133 Milano, ItalySpecchiasol, R&D Section, Via Rizzi 1/3, 37012 Bussolengo, ItalySpecchiasol, R&D Section, Via Rizzi 1/3, 37012 Bussolengo, ItalyDepartment of Food, Environmental and Nutritional Sciences (DeFENS), Università Degli Studi di Milano, Via Celoria 2, 20133 Milano, ItalyConsumers must be assured that bought food supplements contain both bilberry extract and the anthocyanin amounts that match the declared levels. Therefore, a Fourier transform near-infrared (FT-NIR) spectroscopic method was validated based on principal component scores for the prediction of bilberry extract adulteration and partial least squares regression model for total anthocyanin evaluation. Anthocyanins have been quantified individually in 71 commercial bilberry extracts by HPLC-DAD, and 6 of them were counterfeit. The anthocyanin content in bilberry extracts was in the range 18–34%. Authentic bilberry extracts (n=65) were divided into two parts: one for calibration (n=38) and the other for the validation set (n=27). Spectra were recorded in the range of 4000–12500 cm−1, and a good prediction model was obtained in the range of 9400–6096 and 5456–4248 cm−1 with r2 of 99.5% and a root-mean-square error of 0.3%. The adulterated extracts subjected to NIR analysis were recognized as noncompliant, thus confirming the results obtained by chromatography. The FT-NIR spectroscopy is an economic, powerful, and fast methodology for the detection of adulteration and quantification of the total anthocyanin in bilberry extracts; above all, it is a rapid, low cost, and nondestructive technique for routine analysis.http://dx.doi.org/10.1155/2018/4751247 |
| spellingShingle | Claudio Gardana Antonio Scialpi Christian Fachechi Paolo Simonetti Near-Infrared Spectroscopy and Chemometrics for the Routine Detection of Bilberry Extract Adulteration and Quantitative Determination of the Anthocyanins Journal of Spectroscopy |
| title | Near-Infrared Spectroscopy and Chemometrics for the Routine Detection of Bilberry Extract Adulteration and Quantitative Determination of the Anthocyanins |
| title_full | Near-Infrared Spectroscopy and Chemometrics for the Routine Detection of Bilberry Extract Adulteration and Quantitative Determination of the Anthocyanins |
| title_fullStr | Near-Infrared Spectroscopy and Chemometrics for the Routine Detection of Bilberry Extract Adulteration and Quantitative Determination of the Anthocyanins |
| title_full_unstemmed | Near-Infrared Spectroscopy and Chemometrics for the Routine Detection of Bilberry Extract Adulteration and Quantitative Determination of the Anthocyanins |
| title_short | Near-Infrared Spectroscopy and Chemometrics for the Routine Detection of Bilberry Extract Adulteration and Quantitative Determination of the Anthocyanins |
| title_sort | near infrared spectroscopy and chemometrics for the routine detection of bilberry extract adulteration and quantitative determination of the anthocyanins |
| url | http://dx.doi.org/10.1155/2018/4751247 |
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