Facile approach for constructing TEV insertions to probe protein structure in vivo
The tobacco etch virus (TEV) protease has been used as a tool to examine protein structure in vivo. TEV cleavage sites (TEVcs) have been introduced via cloning into unique restriction sites or random transposon mutagenesis. We describe a facile, efficient method for introducing TEVcs at precise loca...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2006-12-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112284 |
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