Characterization of a chemically induced osteoarthritis model in zebrafish
Abstract Osteoarthritis (OA) is characterized by the progressive degeneration of the synovial joint, leading to irreversible damage to articular cartilage and subchondral bone. While animal models have advanced our understanding of OA, numerous unresolved issues still remain. The zebrafish, known fo...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-88125-x |
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| author | Gongyi Xiao Jin Qin Huiping Yang Qizhi Song Ruobin Zhang Junlan Huang Yuexi Mou Wen Liu Xianding Sun Mao Nie |
| author_facet | Gongyi Xiao Jin Qin Huiping Yang Qizhi Song Ruobin Zhang Junlan Huang Yuexi Mou Wen Liu Xianding Sun Mao Nie |
| author_sort | Gongyi Xiao |
| collection | DOAJ |
| description | Abstract Osteoarthritis (OA) is characterized by the progressive degeneration of the synovial joint, leading to irreversible damage to articular cartilage and subchondral bone. While animal models have advanced our understanding of OA, numerous unresolved issues still remain. The zebrafish, known for its transparent body, rapid developmental, and impressive regenerative capabilities, offers substantial potential for osteoarthritis research. This study seeks to establish a new OA model utilizing the zebrafish jaw joint, acting as a supplement to traditional animal models. In the future, this model could serve as a valuable platform for delving deeper into the mechanisms of this disease, as well as for advancing drug discovery and therapeutic interventions. Leveraging the skeletal structure of zebrafish, we targeted the largest jaw joint for our research. A custom fixation device was crafted, and a microinjection system was utilized to inject mono-iodoacetate (MIA) or collagenase type II (CTII) into the joint cavity of zebrafish. Subsequent analyses included histological staining, immunohistochemistry, OA research society international (OARSI) scoring, and real-time in vivo imaging were performed at 7, 14, and 28 days post injection. Our results effectively demonstrated the presence of synovial inflammation and cartilage damage within the zebrafish mandible, affirming the feasibility of inducing OA in zebrafish. In conclusion, the local injection of chemical agents into the joint cavity of zebrafish effectively induced the occurrence of OA. Establishing the zebrafish OA model enhances the array of animal models available for OA research. Moreover, zebrafish present distinct advantages, including robust regenerative abilities, genetic editing simplicity, and efficient drug screening. Consequently, this offers a fresh avenue for investigating the pathogenesis, prevention, and potential therapeutic approaches for human OA. |
| format | Article |
| id | doaj-art-6ca5a52809c149d19597d85859a2d64b |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-6ca5a52809c149d19597d85859a2d64b2025-02-02T12:18:37ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-025-88125-xCharacterization of a chemically induced osteoarthritis model in zebrafishGongyi Xiao0Jin Qin1Huiping Yang2Qizhi Song3Ruobin Zhang4Junlan Huang5Yuexi Mou6Wen Liu7Xianding Sun8Mao Nie9Center for Joint Surgery, Department of Orthopedic Surgery, The Second Affiliated Hospital of Chongqing Medical UniversityCenter for Spinal Surgery, Department of Orthopedic Surgery, The Second Affiliated Hospital of Chongqing Medical UniversityDepartment of Cardiology, The Second Affiliated Hospital of Chongqing Medical UniversityDepartment of Orthopedic Surgery, Chonggang General HospitalDepartment of Wound Repair and Rehabilitation Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Trauma Center, Research Institute of Surgery, Daping Hospital, Army Medical UniversityDepartment of Wound Repair and Rehabilitation Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Trauma Center, Research Institute of Surgery, Daping Hospital, Army Medical UniversityCenter for Joint Surgery, Department of Orthopedic Surgery, The Second Affiliated Hospital of Chongqing Medical UniversityCenter for Joint Surgery, Department of Orthopedic Surgery, The Second Affiliated Hospital of Chongqing Medical UniversityCenter for Joint Surgery, Department of Orthopedic Surgery, The Second Affiliated Hospital of Chongqing Medical UniversityCenter for Joint Surgery, Department of Orthopedic Surgery, The Second Affiliated Hospital of Chongqing Medical UniversityAbstract Osteoarthritis (OA) is characterized by the progressive degeneration of the synovial joint, leading to irreversible damage to articular cartilage and subchondral bone. While animal models have advanced our understanding of OA, numerous unresolved issues still remain. The zebrafish, known for its transparent body, rapid developmental, and impressive regenerative capabilities, offers substantial potential for osteoarthritis research. This study seeks to establish a new OA model utilizing the zebrafish jaw joint, acting as a supplement to traditional animal models. In the future, this model could serve as a valuable platform for delving deeper into the mechanisms of this disease, as well as for advancing drug discovery and therapeutic interventions. Leveraging the skeletal structure of zebrafish, we targeted the largest jaw joint for our research. A custom fixation device was crafted, and a microinjection system was utilized to inject mono-iodoacetate (MIA) or collagenase type II (CTII) into the joint cavity of zebrafish. Subsequent analyses included histological staining, immunohistochemistry, OA research society international (OARSI) scoring, and real-time in vivo imaging were performed at 7, 14, and 28 days post injection. Our results effectively demonstrated the presence of synovial inflammation and cartilage damage within the zebrafish mandible, affirming the feasibility of inducing OA in zebrafish. In conclusion, the local injection of chemical agents into the joint cavity of zebrafish effectively induced the occurrence of OA. Establishing the zebrafish OA model enhances the array of animal models available for OA research. Moreover, zebrafish present distinct advantages, including robust regenerative abilities, genetic editing simplicity, and efficient drug screening. Consequently, this offers a fresh avenue for investigating the pathogenesis, prevention, and potential therapeutic approaches for human OA.https://doi.org/10.1038/s41598-025-88125-xOsteoarthritisZebrafish modelJaw jointMono-iodoacetateCollagenase type II |
| spellingShingle | Gongyi Xiao Jin Qin Huiping Yang Qizhi Song Ruobin Zhang Junlan Huang Yuexi Mou Wen Liu Xianding Sun Mao Nie Characterization of a chemically induced osteoarthritis model in zebrafish Scientific Reports Osteoarthritis Zebrafish model Jaw joint Mono-iodoacetate Collagenase type II |
| title | Characterization of a chemically induced osteoarthritis model in zebrafish |
| title_full | Characterization of a chemically induced osteoarthritis model in zebrafish |
| title_fullStr | Characterization of a chemically induced osteoarthritis model in zebrafish |
| title_full_unstemmed | Characterization of a chemically induced osteoarthritis model in zebrafish |
| title_short | Characterization of a chemically induced osteoarthritis model in zebrafish |
| title_sort | characterization of a chemically induced osteoarthritis model in zebrafish |
| topic | Osteoarthritis Zebrafish model Jaw joint Mono-iodoacetate Collagenase type II |
| url | https://doi.org/10.1038/s41598-025-88125-x |
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