Role of the Kaiso gene in the development of inflammation in Mucin-2 defcient mice
The number of people with inflammatory bowel disease (IBD) is constantly increasing worldwide. The main factors that have effects on the etiology of the disease are genetic, environmental and immunological. However, the mechanism of disease development and effective treatment of IBD have not yet been f...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2019-01-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/1812 |
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| author | E. A. Litvinova K. M. Achasova M. A. Borisova S. V. Zhenilo E. B. Prokhortchouk E. N. Kozhevnikova |
| author_facet | E. A. Litvinova K. M. Achasova M. A. Borisova S. V. Zhenilo E. B. Prokhortchouk E. N. Kozhevnikova |
| author_sort | E. A. Litvinova |
| collection | DOAJ |
| description | The number of people with inflammatory bowel disease (IBD) is constantly increasing worldwide. The main factors that have effects on the etiology of the disease are genetic, environmental and immunological. However, the mechanism of disease development and effective treatment of IBD have not yet been found. Animal models help address these problems. The most popular model is considered to be transgenic models in which individual genes are knocked out. One of such models for the study of IBD are mice with a null mutation of the Muc2 gene encoding the Mucin-2 protein, which is involved in the formation of a protective mucin layer in the small and large intestine. Some of transcription factors that change the expression of intestinal genes are involved in the development of IBD and colorectal cancer. One of such transcription factors is “zinc fnger” domain-containing protein Kaiso which is able to bind to methylated DNA. In this study, we assessed the role of Kaiso in the development of intestinal inflammation using the experimental model of C57BL/6Muc2-/-Kaiso-/-. We have shown that mice with impaired intestinal barrier function that develop processes similar to human IBD also develop inflammatory responses, such as increased expression of Il1, Tnf and Il17a genes. The defciency of the Kaiso transcription factor in Mucin-2 knockout mice causes a decrease in the expression level of only the Cox2 and Tff3 genes. Perhaps a decline in the expression of the gene encoding cyclooxygenase-2 can lead to a decrease in the expression of the antibacterial factor Trefoil factor 3. However, in the experimental model of IBD, Kaiso protein did not play a signifcant role in the regulation of pro-inflammatory cytokines of tumor necrosis factor and interleukins 1 and 17. |
| format | Article |
| id | doaj-art-6c53872d10394315bd634836c95e4e51 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-6c53872d10394315bd634836c95e4e512025-02-01T09:58:07ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592019-01-012281078108310.18699/VJ18.453860Role of the Kaiso gene in the development of inflammation in Mucin-2 defcient miceE. A. Litvinova0K. M. Achasova1M. A. Borisova2S. V. Zhenilo3E. B. Prokhortchouk4E. N. Kozhevnikova5Institute of Cytology and Genetics, SB RASInstitute of Cytology and Genetics, SB RASInstitute of Cytology and Genetics, SB RASFederal Research Centre “Fundamentals of Biotechnology”, RAS, Institute of BioengineeringFederal Research Centre “Fundamentals of Biotechnology”, RAS, Institute of BioengineeringInstitute of Cytology and Genetics, SB RASThe number of people with inflammatory bowel disease (IBD) is constantly increasing worldwide. The main factors that have effects on the etiology of the disease are genetic, environmental and immunological. However, the mechanism of disease development and effective treatment of IBD have not yet been found. Animal models help address these problems. The most popular model is considered to be transgenic models in which individual genes are knocked out. One of such models for the study of IBD are mice with a null mutation of the Muc2 gene encoding the Mucin-2 protein, which is involved in the formation of a protective mucin layer in the small and large intestine. Some of transcription factors that change the expression of intestinal genes are involved in the development of IBD and colorectal cancer. One of such transcription factors is “zinc fnger” domain-containing protein Kaiso which is able to bind to methylated DNA. In this study, we assessed the role of Kaiso in the development of intestinal inflammation using the experimental model of C57BL/6Muc2-/-Kaiso-/-. We have shown that mice with impaired intestinal barrier function that develop processes similar to human IBD also develop inflammatory responses, such as increased expression of Il1, Tnf and Il17a genes. The defciency of the Kaiso transcription factor in Mucin-2 knockout mice causes a decrease in the expression level of only the Cox2 and Tff3 genes. Perhaps a decline in the expression of the gene encoding cyclooxygenase-2 can lead to a decrease in the expression of the antibacterial factor Trefoil factor 3. However, in the experimental model of IBD, Kaiso protein did not play a signifcant role in the regulation of pro-inflammatory cytokines of tumor necrosis factor and interleukins 1 and 17.https://vavilov.elpub.ru/jour/article/view/1812mice, intestine inflammation, mucin-2, kaiso gene |
| spellingShingle | E. A. Litvinova K. M. Achasova M. A. Borisova S. V. Zhenilo E. B. Prokhortchouk E. N. Kozhevnikova Role of the Kaiso gene in the development of inflammation in Mucin-2 defcient mice Вавиловский журнал генетики и селекции mice, intestine inflammation, mucin-2, kaiso gene |
| title | Role of the Kaiso gene in the development of inflammation in Mucin-2 defcient mice |
| title_full | Role of the Kaiso gene in the development of inflammation in Mucin-2 defcient mice |
| title_fullStr | Role of the Kaiso gene in the development of inflammation in Mucin-2 defcient mice |
| title_full_unstemmed | Role of the Kaiso gene in the development of inflammation in Mucin-2 defcient mice |
| title_short | Role of the Kaiso gene in the development of inflammation in Mucin-2 defcient mice |
| title_sort | role of the kaiso gene in the development of inflammation in mucin 2 defcient mice |
| topic | mice, intestine inflammation, mucin-2, kaiso gene |
| url | https://vavilov.elpub.ru/jour/article/view/1812 |
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