Multiplex Mutagenically Separated PCR: Diagnosis of β-Thalassemia and Hemoglobin Variants
A rapid and simple method, termed multiplex mutagenically separated PCR (MSPCR), was developed to detect several molecular defects in the hemoglobin gene in one PCR. This technique, in which differentsize allele-specific primers were used, specifically amplified both normal and mutant alleles of the...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1997-03-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/97223rr03 |
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