The Use of Digital Microscopy to Compare the Thicknesses of Normal Corneas and Ex Vivo Rejected Corneal Grafts with a Focus on the Descemet’s Membrane
Objective. To compare the thickness of corneal layers, specifically the Descemet’s membrane (DM), in normal corneas and in failed grafts due to rejection (FGRs) using the digital histopathology and to propose a model for the measurement of corneal layers using this method. Methods. This is a prospec...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2019/8283175 |
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| author | Taíse Tognon Sabrina Bergeron Christina Mastromonaco Kleyton Barella Adriano Pasqualotti Laura Nunez Francisco Murta Luciene Barbosa de Sousa Mauro Campos Miguel Noel Nascentes Burnier |
| author_facet | Taíse Tognon Sabrina Bergeron Christina Mastromonaco Kleyton Barella Adriano Pasqualotti Laura Nunez Francisco Murta Luciene Barbosa de Sousa Mauro Campos Miguel Noel Nascentes Burnier |
| author_sort | Taíse Tognon |
| collection | DOAJ |
| description | Objective. To compare the thickness of corneal layers, specifically the Descemet’s membrane (DM), in normal corneas and in failed grafts due to rejection (FGRs) using the digital histopathology and to propose a model for the measurement of corneal layers using this method. Methods. This is a prospective, cross-sectional study performed at the MUHC-McGill University Ocular Pathology & Translational Research Laboratory (McGill University, Montreal, Canada). Histopathological sections of 25 normal human corneas and 40 FGRs were fully digitalized and examined. Inclusion criteria: samples diagnosed as normal corneas or FGRs, from patients older than 18 years of age. Exclusion criteria: histopathological sections without adequate tissue or missing epidemiological information. For each sample, the thicknesses of the epithelium, stroma, and DM were acquired. From a perpendicular plane of reference, two central measurements and two nasal and two temporal peripheral measurements were obtained. Results. There were differences between the normal and FGR groups in the mean central thickness of the epithelium (p<0.001), the nasal and temporal stromal regions (p<0.001), and of the DM in the nasal and temporal regions (p<0.001). Compared with the extremities of the sample (nasal and temporal), the mean thickness of the DM in normal corneas was lower in the central region (p<0.001), and this difference was not found in the FGR group. Conclusions. Normal corneas have a thinner epithelium in the central region than the FGR group. In addition, the stroma and DM thicknesses of the nasal and temporal periphery were significantly higher in normal corneas than in those from the FGR group. The digital microscopy protocol applied in this study may be useful for further research studies regarding cornea and other tissues. |
| format | Article |
| id | doaj-art-6a9aaf02b5af41a8925518c96b00bac3 |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-6a9aaf02b5af41a8925518c96b00bac32025-02-03T01:21:44ZengWileyJournal of Ophthalmology2090-004X2090-00582019-01-01201910.1155/2019/82831758283175The Use of Digital Microscopy to Compare the Thicknesses of Normal Corneas and Ex Vivo Rejected Corneal Grafts with a Focus on the Descemet’s MembraneTaíse Tognon0Sabrina Bergeron1Christina Mastromonaco2Kleyton Barella3Adriano Pasqualotti4Laura Nunez5Francisco Murta6Luciene Barbosa de Sousa7Mauro Campos8Miguel Noel Nascentes Burnier9Federal University of São Paulo, MUHC–McGill University Ocular Pathology & Translational Research Laboratory, Penido Burnier Institute, Campinas, BrazilMUHC–McGill University Ocular Pathology & Translational Research Laboratory, Montreal, CanadaMUHC–McGill University Ocular Pathology & Translational Research Laboratory, Montreal, CanadaPenido Burnier Institute, Campinas, BrazilUniversity of Passo Fundo, Lisbon University, Passo Fundo, BrazilUniversité de Montréal, MUHC–McGill University Ocular Pathology & Translational Research Laboratory, Montreal, CanadaLisbon University, The MUHC–McGill University Ocular Pathology & Translational Research Laboratory, Lisbon, PortugalFederal University of São Paulo, Goiás Eye Bank, São Paulo, BrazilFederal University of São Paulo, São Paulo, BrazilMUHC–McGill University Ocular Pathology & Translational Research Laboratory, Federal University of São Paulo, Montreal, CanadaObjective. To compare the thickness of corneal layers, specifically the Descemet’s membrane (DM), in normal corneas and in failed grafts due to rejection (FGRs) using the digital histopathology and to propose a model for the measurement of corneal layers using this method. Methods. This is a prospective, cross-sectional study performed at the MUHC-McGill University Ocular Pathology & Translational Research Laboratory (McGill University, Montreal, Canada). Histopathological sections of 25 normal human corneas and 40 FGRs were fully digitalized and examined. Inclusion criteria: samples diagnosed as normal corneas or FGRs, from patients older than 18 years of age. Exclusion criteria: histopathological sections without adequate tissue or missing epidemiological information. For each sample, the thicknesses of the epithelium, stroma, and DM were acquired. From a perpendicular plane of reference, two central measurements and two nasal and two temporal peripheral measurements were obtained. Results. There were differences between the normal and FGR groups in the mean central thickness of the epithelium (p<0.001), the nasal and temporal stromal regions (p<0.001), and of the DM in the nasal and temporal regions (p<0.001). Compared with the extremities of the sample (nasal and temporal), the mean thickness of the DM in normal corneas was lower in the central region (p<0.001), and this difference was not found in the FGR group. Conclusions. Normal corneas have a thinner epithelium in the central region than the FGR group. In addition, the stroma and DM thicknesses of the nasal and temporal periphery were significantly higher in normal corneas than in those from the FGR group. The digital microscopy protocol applied in this study may be useful for further research studies regarding cornea and other tissues.http://dx.doi.org/10.1155/2019/8283175 |
| spellingShingle | Taíse Tognon Sabrina Bergeron Christina Mastromonaco Kleyton Barella Adriano Pasqualotti Laura Nunez Francisco Murta Luciene Barbosa de Sousa Mauro Campos Miguel Noel Nascentes Burnier The Use of Digital Microscopy to Compare the Thicknesses of Normal Corneas and Ex Vivo Rejected Corneal Grafts with a Focus on the Descemet’s Membrane Journal of Ophthalmology |
| title | The Use of Digital Microscopy to Compare the Thicknesses of Normal Corneas and Ex Vivo Rejected Corneal Grafts with a Focus on the Descemet’s Membrane |
| title_full | The Use of Digital Microscopy to Compare the Thicknesses of Normal Corneas and Ex Vivo Rejected Corneal Grafts with a Focus on the Descemet’s Membrane |
| title_fullStr | The Use of Digital Microscopy to Compare the Thicknesses of Normal Corneas and Ex Vivo Rejected Corneal Grafts with a Focus on the Descemet’s Membrane |
| title_full_unstemmed | The Use of Digital Microscopy to Compare the Thicknesses of Normal Corneas and Ex Vivo Rejected Corneal Grafts with a Focus on the Descemet’s Membrane |
| title_short | The Use of Digital Microscopy to Compare the Thicknesses of Normal Corneas and Ex Vivo Rejected Corneal Grafts with a Focus on the Descemet’s Membrane |
| title_sort | use of digital microscopy to compare the thicknesses of normal corneas and ex vivo rejected corneal grafts with a focus on the descemet s membrane |
| url | http://dx.doi.org/10.1155/2019/8283175 |
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