DetectEV: A functional enzymatic assay to assess integrity and bioactivity of extracellular vesicles

DetectEV: A functional enzymatic assay to assess integrity and bioactivity of extracellular vesicles

Abstract The application of extracellular vesicles (EVs) as therapeutics or nanocarriers in cell‐free therapies necessitates meticulous evaluations of different features, including their identity, bioactivity, batch‐to‐batch reproducibility, and stability. Given the inherent heterogeneity in EV prep...

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Main Authors: Giorgia Adamo, Sabrina Picciotto, Paola Gargano, Angela Paterna, Samuele Raccosta, Estella Rao, Daniele Paolo Romancino, Giulio Ghersi, Mauro Manno, Monica Salamone, Antonella Bongiovanni
Format: Article
Language:English
Published: Wiley 2025-01-01
Series:Journal of Extracellular Vesicles
Subjects:
bioactivity
extracellular vesicles
functional enzymatic assay
integrity
quality check
standardization
Online Access:https://doi.org/10.1002/jev2.70030
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https://doi.org/10.1002/jev2.70030

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