The Effect of the Tongyang Huoxue Recipe (TYHX) on the Ito/IKur in Ischemia/Reperfusion Sinoatrial Node Cells
Background. The transient outward potassium current (Ito) and the ultrarapid delayed rectifier potassium current (IKur) are major potassium currents involved in the repolarization process of sinoatrial node cells (SNCs). Methods and Results. The SNCs of neonatal rats were divided into control, ische...
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| Format: | Article |
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Wiley
2022-01-01
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| Series: | Cardiovascular Therapeutics |
| Online Access: | http://dx.doi.org/10.1155/2022/4114817 |
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| author | Yanli Wang Qiaomin Wu Jinfeng Liu Ruxiu Liu |
| author_facet | Yanli Wang Qiaomin Wu Jinfeng Liu Ruxiu Liu |
| author_sort | Yanli Wang |
| collection | DOAJ |
| description | Background. The transient outward potassium current (Ito) and the ultrarapid delayed rectifier potassium current (IKur) are major potassium currents involved in the repolarization process of sinoatrial node cells (SNCs). Methods and Results. The SNCs of neonatal rats were divided into control, ischemia/reperfusion (I/R), I/R+blank serum, and Tongyang Huoxue recipe (TYHX) serum groups. Ito and IKur were recorded using the whole cell patch-clamp technique, and the current-voltage (I-V), steady-state activation (SSA), steady-state inactivation (SSI), and recovery from inactivation (RFI) curves were plotted, respectively. Compared to the control group, both the peak current density and the current density at the voltage of Ito and IKur decreased obviously in SNCs after simulated I/R, the SSA curves moved right, and the SSI curves moved left. After TYHX was added to the extracellular solution of SNCs, both the peak current density and the current density at the voltage of Ito and IKur increased significantly, the SSA curves moved left, and the SSI curves moved right with a significant difference of V1/2. The recovery from the IKur RFI curves was slightly restored, and the Ito curves did not change. Conclusions. TYHX increases the peak current density, accelerates the activation, and decreases the inactivation of the Ito and IKur. This may be the mechanism of TYHX in shortening the action potential duration of repolarization, which accelerates spontaneous pulsation. |
| format | Article |
| id | doaj-art-6a4661b905ca4b69afbc7f702c8d6bf6 |
| institution | Kabale University |
| issn | 1755-5922 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cardiovascular Therapeutics |
| spelling | doaj-art-6a4661b905ca4b69afbc7f702c8d6bf62025-02-03T05:57:59ZengWileyCardiovascular Therapeutics1755-59222022-01-01202210.1155/2022/4114817The Effect of the Tongyang Huoxue Recipe (TYHX) on the Ito/IKur in Ischemia/Reperfusion Sinoatrial Node CellsYanli Wang0Qiaomin Wu1Jinfeng Liu2Ruxiu Liu3Guang’anmen HospitalGuang’anmen HospitalGuang’anmen HospitalGuang’anmen HospitalBackground. The transient outward potassium current (Ito) and the ultrarapid delayed rectifier potassium current (IKur) are major potassium currents involved in the repolarization process of sinoatrial node cells (SNCs). Methods and Results. The SNCs of neonatal rats were divided into control, ischemia/reperfusion (I/R), I/R+blank serum, and Tongyang Huoxue recipe (TYHX) serum groups. Ito and IKur were recorded using the whole cell patch-clamp technique, and the current-voltage (I-V), steady-state activation (SSA), steady-state inactivation (SSI), and recovery from inactivation (RFI) curves were plotted, respectively. Compared to the control group, both the peak current density and the current density at the voltage of Ito and IKur decreased obviously in SNCs after simulated I/R, the SSA curves moved right, and the SSI curves moved left. After TYHX was added to the extracellular solution of SNCs, both the peak current density and the current density at the voltage of Ito and IKur increased significantly, the SSA curves moved left, and the SSI curves moved right with a significant difference of V1/2. The recovery from the IKur RFI curves was slightly restored, and the Ito curves did not change. Conclusions. TYHX increases the peak current density, accelerates the activation, and decreases the inactivation of the Ito and IKur. This may be the mechanism of TYHX in shortening the action potential duration of repolarization, which accelerates spontaneous pulsation.http://dx.doi.org/10.1155/2022/4114817 |
| spellingShingle | Yanli Wang Qiaomin Wu Jinfeng Liu Ruxiu Liu The Effect of the Tongyang Huoxue Recipe (TYHX) on the Ito/IKur in Ischemia/Reperfusion Sinoatrial Node Cells Cardiovascular Therapeutics |
| title | The Effect of the Tongyang Huoxue Recipe (TYHX) on the Ito/IKur in Ischemia/Reperfusion Sinoatrial Node Cells |
| title_full | The Effect of the Tongyang Huoxue Recipe (TYHX) on the Ito/IKur in Ischemia/Reperfusion Sinoatrial Node Cells |
| title_fullStr | The Effect of the Tongyang Huoxue Recipe (TYHX) on the Ito/IKur in Ischemia/Reperfusion Sinoatrial Node Cells |
| title_full_unstemmed | The Effect of the Tongyang Huoxue Recipe (TYHX) on the Ito/IKur in Ischemia/Reperfusion Sinoatrial Node Cells |
| title_short | The Effect of the Tongyang Huoxue Recipe (TYHX) on the Ito/IKur in Ischemia/Reperfusion Sinoatrial Node Cells |
| title_sort | effect of the tongyang huoxue recipe tyhx on the ito ikur in ischemia reperfusion sinoatrial node cells |
| url | http://dx.doi.org/10.1155/2022/4114817 |
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