High-efficiency thermal asymmetric interlaced PCR for amplification of unknown flanking sequences
Isolation of unknown DNA sequences flanked by known sequences is an important task in molecular biology research. Thermal asymmetric interlaced PCR (TAIL-PCR) is an effective method for this purpose. However, the success rate of the original TAIL-PCR needs to be increased, and it is more desirable t...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2007-11-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112601 |
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