The African swine fever virus p22 inhibits the JAK-STAT signaling pathway by promoting the TAX1BP1-mediated degradation of the type I interferon receptor.
African swine fever virus (ASFV) is the causative agent of African swine fever (ASF), a devastating disease epidemic in Asia and Europe. Large knowledge gaps regarding the biological characteristics of viral structural proteins have severely hindered the development of vaccines against ASF. The p22...
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| Format: | Article |
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Public Library of Science (PLoS)
2025-07-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1013319 |
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| author | Haojie Ren Yanjin Wang Lian-Feng Li Lan-Fang Shi Yu-He Ma Jun-Hao Fan Xiao-Ya Pan Han-Cheng Shao Yuhang Zhang Shichong Han Bo Wan Hua-Ji Qiu Gai-Ping Zhang Su Li Wen-Rui He |
| author_facet | Haojie Ren Yanjin Wang Lian-Feng Li Lan-Fang Shi Yu-He Ma Jun-Hao Fan Xiao-Ya Pan Han-Cheng Shao Yuhang Zhang Shichong Han Bo Wan Hua-Ji Qiu Gai-Ping Zhang Su Li Wen-Rui He |
| author_sort | Haojie Ren |
| collection | DOAJ |
| description | African swine fever virus (ASFV) is the causative agent of African swine fever (ASF), a devastating disease epidemic in Asia and Europe. Large knowledge gaps regarding the biological characteristics of viral structural proteins have severely hindered the development of vaccines against ASF. The p22 protein, an internal envelope membrane protein of ASFV, is one such protein that is yet to be deciphered despite its significance. Here, our results indicated that p22 is not essential for the morphogenesis and replication of ASFV in porcine alveolar macrophages. The ASFV p22 negatively regulates the IFN-β-triggered activation of the Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway. Mechanistically, the ASFV p22 promotes the association of the Tax1-binding protein 1 (TAX1BP1) with the type I IFN receptor 1 (IFNAR1) via its transmembrane region, thereby facilitating the autophagic degradation of IFNAR1 and impairing the host antiviral responses at the initial step of JAK-STAT signaling pathway. These findings clarify the biological functions of p22 in ASFV replication and uncover a novel autophagy degradation mechanism for IFNAR1, which provide a novel theoretical basis for understanding the biological characteristics of ASFV and may contribute to the development of vaccines and antiviral therapies against ASF. |
| format | Article |
| id | doaj-art-69eb02e40f0942de8d91b745838cbce3 |
| institution | Kabale University |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-69eb02e40f0942de8d91b745838cbce32025-08-20T03:28:09ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742025-07-01217e101331910.1371/journal.ppat.1013319The African swine fever virus p22 inhibits the JAK-STAT signaling pathway by promoting the TAX1BP1-mediated degradation of the type I interferon receptor.Haojie RenYanjin WangLian-Feng LiLan-Fang ShiYu-He MaJun-Hao FanXiao-Ya PanHan-Cheng ShaoYuhang ZhangShichong HanBo WanHua-Ji QiuGai-Ping ZhangSu LiWen-Rui HeAfrican swine fever virus (ASFV) is the causative agent of African swine fever (ASF), a devastating disease epidemic in Asia and Europe. Large knowledge gaps regarding the biological characteristics of viral structural proteins have severely hindered the development of vaccines against ASF. The p22 protein, an internal envelope membrane protein of ASFV, is one such protein that is yet to be deciphered despite its significance. Here, our results indicated that p22 is not essential for the morphogenesis and replication of ASFV in porcine alveolar macrophages. The ASFV p22 negatively regulates the IFN-β-triggered activation of the Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway. Mechanistically, the ASFV p22 promotes the association of the Tax1-binding protein 1 (TAX1BP1) with the type I IFN receptor 1 (IFNAR1) via its transmembrane region, thereby facilitating the autophagic degradation of IFNAR1 and impairing the host antiviral responses at the initial step of JAK-STAT signaling pathway. These findings clarify the biological functions of p22 in ASFV replication and uncover a novel autophagy degradation mechanism for IFNAR1, which provide a novel theoretical basis for understanding the biological characteristics of ASFV and may contribute to the development of vaccines and antiviral therapies against ASF.https://doi.org/10.1371/journal.ppat.1013319 |
| spellingShingle | Haojie Ren Yanjin Wang Lian-Feng Li Lan-Fang Shi Yu-He Ma Jun-Hao Fan Xiao-Ya Pan Han-Cheng Shao Yuhang Zhang Shichong Han Bo Wan Hua-Ji Qiu Gai-Ping Zhang Su Li Wen-Rui He The African swine fever virus p22 inhibits the JAK-STAT signaling pathway by promoting the TAX1BP1-mediated degradation of the type I interferon receptor. PLoS Pathogens |
| title | The African swine fever virus p22 inhibits the JAK-STAT signaling pathway by promoting the TAX1BP1-mediated degradation of the type I interferon receptor. |
| title_full | The African swine fever virus p22 inhibits the JAK-STAT signaling pathway by promoting the TAX1BP1-mediated degradation of the type I interferon receptor. |
| title_fullStr | The African swine fever virus p22 inhibits the JAK-STAT signaling pathway by promoting the TAX1BP1-mediated degradation of the type I interferon receptor. |
| title_full_unstemmed | The African swine fever virus p22 inhibits the JAK-STAT signaling pathway by promoting the TAX1BP1-mediated degradation of the type I interferon receptor. |
| title_short | The African swine fever virus p22 inhibits the JAK-STAT signaling pathway by promoting the TAX1BP1-mediated degradation of the type I interferon receptor. |
| title_sort | african swine fever virus p22 inhibits the jak stat signaling pathway by promoting the tax1bp1 mediated degradation of the type i interferon receptor |
| url | https://doi.org/10.1371/journal.ppat.1013319 |
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