Predicting the Microbiome and Metabolome Dynamics of Natural Apple Fermentation Towards the Development of Enhanced Functional Vinegar
Natural vinegar fermentation is a complex process influenced by the interplay between microbial communities and metabolites. This study examined the interplay between the microbiome and the metabolome over a three-month period, with samples collected every ten days. Using Illumina sequencing and chr...
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2024-10-01
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| author | Bruna Leal Maske Ignácio Ruiz Alexander da Silva Vale Vitória de Mello Sampaio Najua Kêmil El Kadri Carlos Ricardo Soccol Gilberto Vinícius Pereira |
| author_facet | Bruna Leal Maske Ignácio Ruiz Alexander da Silva Vale Vitória de Mello Sampaio Najua Kêmil El Kadri Carlos Ricardo Soccol Gilberto Vinícius Pereira |
| author_sort | Bruna Leal Maske |
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| description | Natural vinegar fermentation is a complex process influenced by the interplay between microbial communities and metabolites. This study examined the interplay between the microbiome and the metabolome over a three-month period, with samples collected every ten days. Using Illumina sequencing and chromatographic techniques (HPLC and GC-MS), we mapped microbial shifts and metabolite profiles. Early fermentation showed a diverse microbial presence, including genera such as <i>Cronobacter</i>, <i>Luteibacter</i>, and <i>Saccharomyces</i>. A stable microbial ecosystem established between days 15 and 70, characterized by the dominance of <i>Leuconostoc</i>, <i>Gluconobacter</i>, and <i>Saccharomyces</i>, which facilitated consistent substrate consumption and metabolite production, including various organic acids and ethanol. By day 70, Acetobacter prevalence increased significantly, correlating with a peak acetic acid production of 12.4 g/L. Correlation analyses revealed significant relationships between specific microbes and volatile organic compounds. This study highlights the crucial roles of these microbes in developing sensory profiles suited for industrial applications and proposes an optimal microbial consortium for enhancing vinegar quality. These data suggest that an optimal microbial consortium for vinegar fermentation should include <i>Saccharomyces</i> for efficient alcohol production, <i>Leuconostoc</i> for ester-mediated flavor complexity, and <i>Acetobacter</i> for robust acetic acid production. The presence of <i>Komagataeibacter</i> could further improve the sensory and functional qualities due to its role in producing bacterial cellulose. |
| format | Article |
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| publishDate | 2024-10-01 |
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| series | Fermentation |
| spelling | doaj-art-69dbafaa995c4049afa53cec16a84e4a2025-08-20T02:08:15ZengMDPI AGFermentation2311-56372024-10-01101155210.3390/fermentation10110552Predicting the Microbiome and Metabolome Dynamics of Natural Apple Fermentation Towards the Development of Enhanced Functional VinegarBruna Leal Maske0Ignácio Ruiz1Alexander da Silva Vale2Vitória de Mello Sampaio3Najua Kêmil El Kadri4Carlos Ricardo Soccol5Gilberto Vinícius Pereira6Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), Curitiba 81531-990, PR, BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), Curitiba 81531-990, PR, BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), Curitiba 81531-990, PR, BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), Curitiba 81531-990, PR, BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), Curitiba 81531-990, PR, BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), Curitiba 81531-990, PR, BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), Curitiba 81531-990, PR, BrazilNatural vinegar fermentation is a complex process influenced by the interplay between microbial communities and metabolites. This study examined the interplay between the microbiome and the metabolome over a three-month period, with samples collected every ten days. Using Illumina sequencing and chromatographic techniques (HPLC and GC-MS), we mapped microbial shifts and metabolite profiles. Early fermentation showed a diverse microbial presence, including genera such as <i>Cronobacter</i>, <i>Luteibacter</i>, and <i>Saccharomyces</i>. A stable microbial ecosystem established between days 15 and 70, characterized by the dominance of <i>Leuconostoc</i>, <i>Gluconobacter</i>, and <i>Saccharomyces</i>, which facilitated consistent substrate consumption and metabolite production, including various organic acids and ethanol. By day 70, Acetobacter prevalence increased significantly, correlating with a peak acetic acid production of 12.4 g/L. Correlation analyses revealed significant relationships between specific microbes and volatile organic compounds. This study highlights the crucial roles of these microbes in developing sensory profiles suited for industrial applications and proposes an optimal microbial consortium for enhancing vinegar quality. These data suggest that an optimal microbial consortium for vinegar fermentation should include <i>Saccharomyces</i> for efficient alcohol production, <i>Leuconostoc</i> for ester-mediated flavor complexity, and <i>Acetobacter</i> for robust acetic acid production. The presence of <i>Komagataeibacter</i> could further improve the sensory and functional qualities due to its role in producing bacterial cellulose.https://www.mdpi.com/2311-5637/10/11/552LAB<i>Lactobacillus</i>acetic acidnatural fermentation |
| spellingShingle | Bruna Leal Maske Ignácio Ruiz Alexander da Silva Vale Vitória de Mello Sampaio Najua Kêmil El Kadri Carlos Ricardo Soccol Gilberto Vinícius Pereira Predicting the Microbiome and Metabolome Dynamics of Natural Apple Fermentation Towards the Development of Enhanced Functional Vinegar Fermentation LAB <i>Lactobacillus</i> acetic acid natural fermentation |
| title | Predicting the Microbiome and Metabolome Dynamics of Natural Apple Fermentation Towards the Development of Enhanced Functional Vinegar |
| title_full | Predicting the Microbiome and Metabolome Dynamics of Natural Apple Fermentation Towards the Development of Enhanced Functional Vinegar |
| title_fullStr | Predicting the Microbiome and Metabolome Dynamics of Natural Apple Fermentation Towards the Development of Enhanced Functional Vinegar |
| title_full_unstemmed | Predicting the Microbiome and Metabolome Dynamics of Natural Apple Fermentation Towards the Development of Enhanced Functional Vinegar |
| title_short | Predicting the Microbiome and Metabolome Dynamics of Natural Apple Fermentation Towards the Development of Enhanced Functional Vinegar |
| title_sort | predicting the microbiome and metabolome dynamics of natural apple fermentation towards the development of enhanced functional vinegar |
| topic | LAB <i>Lactobacillus</i> acetic acid natural fermentation |
| url | https://www.mdpi.com/2311-5637/10/11/552 |
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