Combined transcriptome and metabolome analysis of stable knockdown and overexpression of the CD8A gene in chicken T lymphocytes
CD8 subunit alpha (CD8A) is an important gene in immunity and is involved in the functional regulation of T lymphocytes. However, the specific role and regulatory mechanism of CD8A in chicken T lymphocytes remain unknown. In this study, we overexpressed and interfered with CD8A in chicken T lymphocy...
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Elsevier
2025-02-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124012641 |
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| author | Yanli Du Kun Wang Xiannian Zi Xiao Wang Meiquan Li Bo Zhang Jinshan Ran Wei Huang Jing Wang Cuilian Dong Hanyi Xiang Li Lei Changrong Ge Yong Liu |
| author_facet | Yanli Du Kun Wang Xiannian Zi Xiao Wang Meiquan Li Bo Zhang Jinshan Ran Wei Huang Jing Wang Cuilian Dong Hanyi Xiang Li Lei Changrong Ge Yong Liu |
| author_sort | Yanli Du |
| collection | DOAJ |
| description | CD8 subunit alpha (CD8A) is an important gene in immunity and is involved in the functional regulation of T lymphocytes. However, the specific role and regulatory mechanism of CD8A in chicken T lymphocytes remain unknown. In this study, we overexpressed and interfered with CD8A in chicken T lymphocytes and found that interfering with CD8A expression inhibited the proliferation and induced the apoptosis of T lymphocytes and that the overexpression of CD8A promoted T lymphocyte activation. Additionally, transcriptomic and metabolomic analyses of chicken T lymphocytes with CD8A overexpression or interference were performed. The overexpression and interference of the CD8A gene caused widespread changes in gene and metabolite expression in chicken T cells. The results of the transcriptome analysis revealed that differentially expressed genes (DEGs) caused by altered expression of the CD8A gene were associated with multiple “neuroactive ligand-receptor interaction”, “cell adhesion molecules”, “calcium signaling pathway”, etc. The metabolome analysis results revealed that different metabolites (DMs) caused by altered CD8A gene expression were associated with “Glutathione metabolism”, “Arginine biosynthesis”, “D-amino acid metabolism”, etc. The combined transcriptional and metabolic analysis revealed one metabolically related pathway, “Glutathione metabolism”. Our findings further revealed that interference and overexpression of CD8A plays a role in the metabolism of Glutathione. Thus, CD8A may be a critical regulator of “Glutathione metabolism” and may subsequently affect T-cell function in chickens. These results provide an important reference for further research on the effect of CD8A on the immune performance of chickens. |
| format | Article |
| id | doaj-art-679df3544bbc483c8dc757cf34ac1ba7 |
| institution | DOAJ |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-679df3544bbc483c8dc757cf34ac1ba72025-08-20T02:50:44ZengElsevierPoultry Science0032-57912025-02-01104210468610.1016/j.psj.2024.104686Combined transcriptome and metabolome analysis of stable knockdown and overexpression of the CD8A gene in chicken T lymphocytesYanli Du0Kun Wang1Xiannian Zi2Xiao Wang3Meiquan Li4Bo Zhang5Jinshan Ran6Wei Huang7Jing Wang8Cuilian Dong9Hanyi Xiang10Li Lei11Changrong Ge12Yong Liu13College of Agronomy and Life Sciences, Kunming University, Kunming 650200, Yunnan Province, PR ChinaCollege of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan Province, PR ChinaCollege of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan Province, PR ChinaCollege of Agronomy and Life Sciences, Kunming University, Kunming 650200, Yunnan Province, PR ChinaCollege of Agronomy and Life Sciences, Kunming University, Kunming 650200, Yunnan Province, PR ChinaCollege of Agronomy and Life Sciences, Kunming University, Kunming 650200, Yunnan Province, PR ChinaCollege of Agronomy and Life Sciences, Kunming University, Kunming 650200, Yunnan Province, PR ChinaCollege of Agronomy and Life Sciences, Kunming University, Kunming 650200, Yunnan Province, PR ChinaCollege of Agronomy and Life Sciences, Kunming University, Kunming 650200, Yunnan Province, PR ChinaCollege of Agronomy and Life Sciences, Kunming University, Kunming 650200, Yunnan Province, PR ChinaYunnan Rural Revitalizing Education Institute, Yunnan Open University, Kunming 650101, Yunnan Province, PR ChinaYunnan Rural Revitalizing Education Institute, Yunnan Open University, Kunming 650101, Yunnan Province, PR ChinaCollege of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan Province, PR ChinaCorresponding author:.; Yunnan Rural Revitalizing Education Institute, Yunnan Open University, Kunming 650101, Yunnan Province, PR China; Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Guangxi Bufialo Research, Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, PR China; College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan Province, PR ChinaCD8 subunit alpha (CD8A) is an important gene in immunity and is involved in the functional regulation of T lymphocytes. However, the specific role and regulatory mechanism of CD8A in chicken T lymphocytes remain unknown. In this study, we overexpressed and interfered with CD8A in chicken T lymphocytes and found that interfering with CD8A expression inhibited the proliferation and induced the apoptosis of T lymphocytes and that the overexpression of CD8A promoted T lymphocyte activation. Additionally, transcriptomic and metabolomic analyses of chicken T lymphocytes with CD8A overexpression or interference were performed. The overexpression and interference of the CD8A gene caused widespread changes in gene and metabolite expression in chicken T cells. The results of the transcriptome analysis revealed that differentially expressed genes (DEGs) caused by altered expression of the CD8A gene were associated with multiple “neuroactive ligand-receptor interaction”, “cell adhesion molecules”, “calcium signaling pathway”, etc. The metabolome analysis results revealed that different metabolites (DMs) caused by altered CD8A gene expression were associated with “Glutathione metabolism”, “Arginine biosynthesis”, “D-amino acid metabolism”, etc. The combined transcriptional and metabolic analysis revealed one metabolically related pathway, “Glutathione metabolism”. Our findings further revealed that interference and overexpression of CD8A plays a role in the metabolism of Glutathione. Thus, CD8A may be a critical regulator of “Glutathione metabolism” and may subsequently affect T-cell function in chickens. These results provide an important reference for further research on the effect of CD8A on the immune performance of chickens.http://www.sciencedirect.com/science/article/pii/S0032579124012641CD8AInterferenceOverexpressionTranscriptome analysisMetabolome analysis |
| spellingShingle | Yanli Du Kun Wang Xiannian Zi Xiao Wang Meiquan Li Bo Zhang Jinshan Ran Wei Huang Jing Wang Cuilian Dong Hanyi Xiang Li Lei Changrong Ge Yong Liu Combined transcriptome and metabolome analysis of stable knockdown and overexpression of the CD8A gene in chicken T lymphocytes Poultry Science CD8A Interference Overexpression Transcriptome analysis Metabolome analysis |
| title | Combined transcriptome and metabolome analysis of stable knockdown and overexpression of the CD8A gene in chicken T lymphocytes |
| title_full | Combined transcriptome and metabolome analysis of stable knockdown and overexpression of the CD8A gene in chicken T lymphocytes |
| title_fullStr | Combined transcriptome and metabolome analysis of stable knockdown and overexpression of the CD8A gene in chicken T lymphocytes |
| title_full_unstemmed | Combined transcriptome and metabolome analysis of stable knockdown and overexpression of the CD8A gene in chicken T lymphocytes |
| title_short | Combined transcriptome and metabolome analysis of stable knockdown and overexpression of the CD8A gene in chicken T lymphocytes |
| title_sort | combined transcriptome and metabolome analysis of stable knockdown and overexpression of the cd8a gene in chicken t lymphocytes |
| topic | CD8A Interference Overexpression Transcriptome analysis Metabolome analysis |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124012641 |
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