Endogenous plasma resuspension of peripheral blood mononuclear cells prevents preparative-associated stress that modifies polyA-enriched RNA responses to subsequent acute stressors

Endogenous plasma resuspension of peripheral blood mononuclear cells prevents preparative-associated stress that modifies polyA-enriched RNA responses to subsequent acute stressors

Human peripheral blood mononuclear cells (PBMCs) are used to examine biological processes and disease, when basal variability in cellular activation and splicing is described and unexplained. Using isolation systems that maintained buffy coat cells (PBMCs, platelets) in their own plasma, poly-A enri...

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Bibliographic Details
Main Authors: Dongyang Li, Karina Al-Dahleh, Daniel A Murphy, Sonya Georgieva, Nik Matthews, Claire L Shovlin
Format: Article
Language:English
Published: Shared Science Publishers OG 2024-11-01
Series:Cell Stress
Subjects:
cycloheximide
hypothermia
integrated stress response
intronless (single exon) genes
iron
protein translation inhibition
reactive oxygen species
poly-a selected rnaseq
experimental variability
Online Access:http://microbialcell.com/researcharticles/2024a-li-cell-stress
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