Postharvest Application of <i>Myo</i>-Inositol Extends the Shelf-Life of Banana Fruit by Delaying Ethylene Biosynthesis and Improving Antioxidant Activity

Banana fruits are harvested and then undergo rapid ripening and senescence, sharply limiting their shelf-life and marketability. <i>Myo</i>-inositol (MI) is an important regulator in ethylene production and reactive oxygen species (ROS) accumulation; however, its involvement in the posth...

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Main Authors: Lingyu Hu, Yi Li, Kun Zhou, Kaili Shi, Yi Niu, Feng Qu, Shenglin Zhang, Weidi He, Yuanli Wu
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Foods
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Online Access:https://www.mdpi.com/2304-8158/14/15/2638
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author Lingyu Hu
Yi Li
Kun Zhou
Kaili Shi
Yi Niu
Feng Qu
Shenglin Zhang
Weidi He
Yuanli Wu
author_facet Lingyu Hu
Yi Li
Kun Zhou
Kaili Shi
Yi Niu
Feng Qu
Shenglin Zhang
Weidi He
Yuanli Wu
author_sort Lingyu Hu
collection DOAJ
description Banana fruits are harvested and then undergo rapid ripening and senescence, sharply limiting their shelf-life and marketability. <i>Myo</i>-inositol (MI) is an important regulator in ethylene production and reactive oxygen species (ROS) accumulation; however, its involvement in the postharvest ripening process of banana remains to be determined. This study found that postharvest application of MI could efficiently delay the fruit ripening and extend the time in which the luster, color, and hardness were maintained in two cultivars with contrasting storage characteristics, storable ‘Brazil’ and unstorable ‘Fenza No. 1’, when stored at room temperature (23 °C ± 2 °C). Moreover, physiological, metabolic, and gene expression analyses indicated that MI application improved MI metabolism and postponed ethylene biosynthesis and cell wall loosening. The decrease in ethylene production was associated with a reduction in the expression of <i>ACS1</i> and <i>ACO1</i> genes. MI treatment decreased the expressions of <i>PL1/2</i>, <i>PG</i>, and <i>EXP1/7/8</i>, which may account for the delay in softening. In addition, the application of MI could alleviate ROS-mediated senescence and cell membrane damage by promoting the activities of SOD, POD, and anti-O<sub>2</sub><sup>−</sup> and decreasing PPO activity. This study shed light on the function of MI in regulating the postharvest ripening and senescence of bananas and provided an efficient strategy for extending shelf-life and reduce losses.
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institution Kabale University
issn 2304-8158
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publishDate 2025-07-01
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series Foods
spelling doaj-art-66b2a2dbeedd4d8ba3e1eb038be2963d2025-08-20T04:00:49ZengMDPI AGFoods2304-81582025-07-011415263810.3390/foods14152638Postharvest Application of <i>Myo</i>-Inositol Extends the Shelf-Life of Banana Fruit by Delaying Ethylene Biosynthesis and Improving Antioxidant ActivityLingyu Hu0Yi Li1Kun Zhou2Kaili Shi3Yi Niu4Feng Qu5Shenglin Zhang6Weidi He7Yuanli Wu8College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, ChinaCollege of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, ChinaCollege of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, ChinaCollege of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, ChinaCollege of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, ChinaCollege of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, ChinaCollege of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, ChinaInstitute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs Guangdong Provincial Key Laboratory of Science and Technology Research on Fruit Tree, Guangzhou 510640, ChinaInstitute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs Guangdong Provincial Key Laboratory of Science and Technology Research on Fruit Tree, Guangzhou 510640, ChinaBanana fruits are harvested and then undergo rapid ripening and senescence, sharply limiting their shelf-life and marketability. <i>Myo</i>-inositol (MI) is an important regulator in ethylene production and reactive oxygen species (ROS) accumulation; however, its involvement in the postharvest ripening process of banana remains to be determined. This study found that postharvest application of MI could efficiently delay the fruit ripening and extend the time in which the luster, color, and hardness were maintained in two cultivars with contrasting storage characteristics, storable ‘Brazil’ and unstorable ‘Fenza No. 1’, when stored at room temperature (23 °C ± 2 °C). Moreover, physiological, metabolic, and gene expression analyses indicated that MI application improved MI metabolism and postponed ethylene biosynthesis and cell wall loosening. The decrease in ethylene production was associated with a reduction in the expression of <i>ACS1</i> and <i>ACO1</i> genes. MI treatment decreased the expressions of <i>PL1/2</i>, <i>PG</i>, and <i>EXP1/7/8</i>, which may account for the delay in softening. In addition, the application of MI could alleviate ROS-mediated senescence and cell membrane damage by promoting the activities of SOD, POD, and anti-O<sub>2</sub><sup>−</sup> and decreasing PPO activity. This study shed light on the function of MI in regulating the postharvest ripening and senescence of bananas and provided an efficient strategy for extending shelf-life and reduce losses.https://www.mdpi.com/2304-8158/14/15/2638banana ripeningshelf-life<i>myo</i>-inositolethylene biosynthesisreactive oxygen speciescell wall loosening
spellingShingle Lingyu Hu
Yi Li
Kun Zhou
Kaili Shi
Yi Niu
Feng Qu
Shenglin Zhang
Weidi He
Yuanli Wu
Postharvest Application of <i>Myo</i>-Inositol Extends the Shelf-Life of Banana Fruit by Delaying Ethylene Biosynthesis and Improving Antioxidant Activity
Foods
banana ripening
shelf-life
<i>myo</i>-inositol
ethylene biosynthesis
reactive oxygen species
cell wall loosening
title Postharvest Application of <i>Myo</i>-Inositol Extends the Shelf-Life of Banana Fruit by Delaying Ethylene Biosynthesis and Improving Antioxidant Activity
title_full Postharvest Application of <i>Myo</i>-Inositol Extends the Shelf-Life of Banana Fruit by Delaying Ethylene Biosynthesis and Improving Antioxidant Activity
title_fullStr Postharvest Application of <i>Myo</i>-Inositol Extends the Shelf-Life of Banana Fruit by Delaying Ethylene Biosynthesis and Improving Antioxidant Activity
title_full_unstemmed Postharvest Application of <i>Myo</i>-Inositol Extends the Shelf-Life of Banana Fruit by Delaying Ethylene Biosynthesis and Improving Antioxidant Activity
title_short Postharvest Application of <i>Myo</i>-Inositol Extends the Shelf-Life of Banana Fruit by Delaying Ethylene Biosynthesis and Improving Antioxidant Activity
title_sort postharvest application of i myo i inositol extends the shelf life of banana fruit by delaying ethylene biosynthesis and improving antioxidant activity
topic banana ripening
shelf-life
<i>myo</i>-inositol
ethylene biosynthesis
reactive oxygen species
cell wall loosening
url https://www.mdpi.com/2304-8158/14/15/2638
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