Metal ion-crosslinking multifunctional hydrogel microspheres with inflammatory immune regulation for cartilage regeneration
IntroductionOsteoarthritis (OA) is a degenerative disease of the joints characterized by cartilage degradation and synovial inflammation. Due to the complex pathogenesis of OA, multifaceted therapies that modulate inflammatory and immune microenvironmental disturbances while promoting cartilage rege...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1540592/full |
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| author | Zhuoming Xu Zhuoming Xu Jun Ma Hanyin Hu Hanyin Hu Jintao Liu Jintao Liu Haiyang Yang Haiyang Yang Jiayi Chen Hongwei Xu Xinyu Wang Huanhuan Luo Gang Chen |
| author_facet | Zhuoming Xu Zhuoming Xu Jun Ma Hanyin Hu Hanyin Hu Jintao Liu Jintao Liu Haiyang Yang Haiyang Yang Jiayi Chen Hongwei Xu Xinyu Wang Huanhuan Luo Gang Chen |
| author_sort | Zhuoming Xu |
| collection | DOAJ |
| description | IntroductionOsteoarthritis (OA) is a degenerative disease of the joints characterized by cartilage degradation and synovial inflammation. Due to the complex pathogenesis of OA, multifaceted therapies that modulate inflammatory and immune microenvironmental disturbances while promoting cartilage regeneration are key to control the progression of OA.MethodsHerein, a multifunctional nanoparticle (DIC/Mg-PDA NPs) was constructed successfully by the metal chelation effect between Mg2+ and catecholamine bond from dopamine, followed by the amidation with diclofenac (DIC), which was then prepared into an injectable hydrogel microsphere (DIC/Mg-PDA@HM) with immune-regulating and cartilage-repairing abilities through microfluidic technology for the treatment of osteoarthritis.Results and discussionThe sustained release of Mg2+ from the composite hydrogel microspheres achieved inflammatory immune regulation by converting macrophages from M1 to M2 and promoted cartilage regeneration through the differentiation of BMSCs. Moreover, the enhanced release of DIC and polydopamine (PDA) effectively downregulated inflammatory factors, and finally achieved OA therapy. In addition, in vivo MRI and tissue section staining of OA model proved the significant efficacy of the hydrogel microspheres on OA. In conclusion, these novel hydrogel microspheres demonstrated a promising prospect for multidisciplinary repairing of OA. |
| format | Article |
| id | doaj-art-66abb6985405410f849fe565e003f399 |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-66abb6985405410f849fe565e003f3992025-01-28T06:41:17ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-01-011310.3389/fbioe.2025.15405921540592Metal ion-crosslinking multifunctional hydrogel microspheres with inflammatory immune regulation for cartilage regenerationZhuoming Xu0Zhuoming Xu1Jun Ma2Hanyin Hu3Hanyin Hu4Jintao Liu5Jintao Liu6Haiyang Yang7Haiyang Yang8Jiayi Chen9Hongwei Xu10Xinyu Wang11Huanhuan Luo12Gang Chen13Jiaxing University Master Degree Cultivation Base, Zhejiang Chinese Medical University, Hangzhou, ChinaDepartment of Orthopaedics, Jiaxing Key Laboratory of Basic Research and Clinical Translation on Orthopedic Biomaterials, The Second Affiliated Hospital of Jiaxing University, Jiaxing, ChinaDepartment of Orthopaedics, Jiaxing Key Laboratory of Basic Research and Clinical Translation on Orthopedic Biomaterials, The Second Affiliated Hospital of Jiaxing University, Jiaxing, ChinaJiaxing University Master Degree Cultivation Base, Zhejiang Chinese Medical University, Hangzhou, ChinaDepartment of Orthopaedics, Jiaxing Key Laboratory of Basic Research and Clinical Translation on Orthopedic Biomaterials, The Second Affiliated Hospital of Jiaxing University, Jiaxing, ChinaJiaxing University Master Degree Cultivation Base, Zhejiang Chinese Medical University, Hangzhou, ChinaDepartment of Orthopaedics, Jiaxing Key Laboratory of Basic Research and Clinical Translation on Orthopedic Biomaterials, The Second Affiliated Hospital of Jiaxing University, Jiaxing, ChinaJiaxing University Master Degree Cultivation Base, Zhejiang Chinese Medical University, Hangzhou, ChinaDepartment of Orthopaedics, Jiaxing Key Laboratory of Basic Research and Clinical Translation on Orthopedic Biomaterials, The Second Affiliated Hospital of Jiaxing University, Jiaxing, ChinaDepartment of Orthopaedics, Jiaxing Key Laboratory of Basic Research and Clinical Translation on Orthopedic Biomaterials, The Second Affiliated Hospital of Jiaxing University, Jiaxing, ChinaDepartment of Orthopaedics, Jiaxing Key Laboratory of Basic Research and Clinical Translation on Orthopedic Biomaterials, The Second Affiliated Hospital of Jiaxing University, Jiaxing, ChinaDepartment of Radiology, The Second Affiliated Hospital of Jiaxing University, Jiaxing, ChinaDepartment of Orthopaedics, Jiaxing Key Laboratory of Basic Research and Clinical Translation on Orthopedic Biomaterials, The Second Affiliated Hospital of Jiaxing University, Jiaxing, ChinaDepartment of Orthopaedics, Jiaxing Key Laboratory of Basic Research and Clinical Translation on Orthopedic Biomaterials, The Second Affiliated Hospital of Jiaxing University, Jiaxing, ChinaIntroductionOsteoarthritis (OA) is a degenerative disease of the joints characterized by cartilage degradation and synovial inflammation. Due to the complex pathogenesis of OA, multifaceted therapies that modulate inflammatory and immune microenvironmental disturbances while promoting cartilage regeneration are key to control the progression of OA.MethodsHerein, a multifunctional nanoparticle (DIC/Mg-PDA NPs) was constructed successfully by the metal chelation effect between Mg2+ and catecholamine bond from dopamine, followed by the amidation with diclofenac (DIC), which was then prepared into an injectable hydrogel microsphere (DIC/Mg-PDA@HM) with immune-regulating and cartilage-repairing abilities through microfluidic technology for the treatment of osteoarthritis.Results and discussionThe sustained release of Mg2+ from the composite hydrogel microspheres achieved inflammatory immune regulation by converting macrophages from M1 to M2 and promoted cartilage regeneration through the differentiation of BMSCs. Moreover, the enhanced release of DIC and polydopamine (PDA) effectively downregulated inflammatory factors, and finally achieved OA therapy. In addition, in vivo MRI and tissue section staining of OA model proved the significant efficacy of the hydrogel microspheres on OA. In conclusion, these novel hydrogel microspheres demonstrated a promising prospect for multidisciplinary repairing of OA.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1540592/fullhydrogel microspheremagnesium ioncartilage regenerationosteoarthritisanti-inflammatoryimmunomodulation |
| spellingShingle | Zhuoming Xu Zhuoming Xu Jun Ma Hanyin Hu Hanyin Hu Jintao Liu Jintao Liu Haiyang Yang Haiyang Yang Jiayi Chen Hongwei Xu Xinyu Wang Huanhuan Luo Gang Chen Metal ion-crosslinking multifunctional hydrogel microspheres with inflammatory immune regulation for cartilage regeneration Frontiers in Bioengineering and Biotechnology hydrogel microsphere magnesium ion cartilage regeneration osteoarthritis anti-inflammatory immunomodulation |
| title | Metal ion-crosslinking multifunctional hydrogel microspheres with inflammatory immune regulation for cartilage regeneration |
| title_full | Metal ion-crosslinking multifunctional hydrogel microspheres with inflammatory immune regulation for cartilage regeneration |
| title_fullStr | Metal ion-crosslinking multifunctional hydrogel microspheres with inflammatory immune regulation for cartilage regeneration |
| title_full_unstemmed | Metal ion-crosslinking multifunctional hydrogel microspheres with inflammatory immune regulation for cartilage regeneration |
| title_short | Metal ion-crosslinking multifunctional hydrogel microspheres with inflammatory immune regulation for cartilage regeneration |
| title_sort | metal ion crosslinking multifunctional hydrogel microspheres with inflammatory immune regulation for cartilage regeneration |
| topic | hydrogel microsphere magnesium ion cartilage regeneration osteoarthritis anti-inflammatory immunomodulation |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1540592/full |
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