Synthesis of 1,2,4-Oxadiazin-5(6<i>H</i>)-One Derivatives and Their Biological Investigation as Monoamine Oxidase Inhibitors

Monoamine oxidase (MAO) plays a key role in the pathogenesis of central nervous system disorders, and MAO inhibitors have been used in the treatment of depression and Parkinson’s disease. In the search for new classes of MAO inhibitors, the present study investigated a series of 1,2,4-oxadiazin-5(6&...

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Main Authors: Sofia I. Presnukhina, Valentina D. Kotlyarova, Anton A. Shetnev, Sergey V. Baykov, Rakhymzhan Turmanov, Nurbol Appazov, Rakhmetulla Zhapparbergenov, Leilya Zhussupova, Nurila Togyzbayeva, Stephanus J. Cloete, Mikhail K. Korsakov, Vadim P. Boyarskiy, Anél Petzer, Jacobus P. Petzer
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/29/23/5550
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author Sofia I. Presnukhina
Valentina D. Kotlyarova
Anton A. Shetnev
Sergey V. Baykov
Rakhymzhan Turmanov
Nurbol Appazov
Rakhmetulla Zhapparbergenov
Leilya Zhussupova
Nurila Togyzbayeva
Stephanus J. Cloete
Mikhail K. Korsakov
Vadim P. Boyarskiy
Anél Petzer
Jacobus P. Petzer
author_facet Sofia I. Presnukhina
Valentina D. Kotlyarova
Anton A. Shetnev
Sergey V. Baykov
Rakhymzhan Turmanov
Nurbol Appazov
Rakhmetulla Zhapparbergenov
Leilya Zhussupova
Nurila Togyzbayeva
Stephanus J. Cloete
Mikhail K. Korsakov
Vadim P. Boyarskiy
Anél Petzer
Jacobus P. Petzer
author_sort Sofia I. Presnukhina
collection DOAJ
description Monoamine oxidase (MAO) plays a key role in the pathogenesis of central nervous system disorders, and MAO inhibitors have been used in the treatment of depression and Parkinson’s disease. In the search for new classes of MAO inhibitors, the present study investigated a series of 1,2,4-oxadiazin-5(6<i>H</i>)-one derivatives. This study provides the first optimization of the reaction conditions for the condensation of amidoximes with alkyl 2-halocarboxylates to yield the desired 1,2,4-oxadiazin-5(6<i>H</i>)-ones. The results of the in vitro MAO inhibition studies showed that the 1,2,4-oxadiazin-5(6<i>H</i>)-ones were indeed inhibitors of human MAO with the most potent inhibition observed for <b>5f</b> (IC<sub>50</sub> = 0.900 µM) and <b>7c</b> (IC<sub>50</sub> = 0.371 µM). It was concluded that, with appropriate substitution, 1,2,4-oxadiazin-5(6<i>H</i>)-one derivatives would act as good potency MAO-B inhibitors and lead compounds for the development of antiparkinsonian drugs. In Parkinson’s disease, MAO-B inhibitors enhance central dopamine levels and reduce MAO-mediated production of hydrogen peroxide and resultant oxidative injury. This study represents one of few works to investigate synthetic approaches and biological activities of the 1,2,4-oxadiazin-5(6<i>H</i>)-one class of heterocycles.
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spelling doaj-art-666d1696e3a345529b3f8441fd9f2dec2025-08-20T02:38:45ZengMDPI AGMolecules1420-30492024-11-012923555010.3390/molecules29235550Synthesis of 1,2,4-Oxadiazin-5(6<i>H</i>)-One Derivatives and Their Biological Investigation as Monoamine Oxidase InhibitorsSofia I. Presnukhina0Valentina D. Kotlyarova1Anton A. Shetnev2Sergey V. Baykov3Rakhymzhan Turmanov4Nurbol Appazov5Rakhmetulla Zhapparbergenov6Leilya Zhussupova7Nurila Togyzbayeva8Stephanus J. Cloete9Mikhail K. Korsakov10Vadim P. Boyarskiy11Anél Petzer12Jacobus P. Petzer13Institute of Chemistry, Saint Petersburg State University, Universitetskaya Nab., 7/9, 199034 Saint Petersburg, RussiaPharmaceutical Technology Transfer Centre, Yaroslavl State Pedagogical University Named after K.D. Ushinsky, Respublikanskaya St., 108, 150000 Yaroslavl, RussiaLaboratory of Engineering Profile “Physical and Chemical Methods of Analysis”, Korkyt Ata Kyzylorda University, 29 Aiteke Bi Str., Kyzylorda 120014, KazakhstanInstitute of Chemistry, Saint Petersburg State University, Universitetskaya Nab., 7/9, 199034 Saint Petersburg, RussiaLaboratory of Engineering Profile “Physical and Chemical Methods of Analysis”, Korkyt Ata Kyzylorda University, 29 Aiteke Bi Str., Kyzylorda 120014, KazakhstanLaboratory of Engineering Profile “Physical and Chemical Methods of Analysis”, Korkyt Ata Kyzylorda University, 29 Aiteke Bi Str., Kyzylorda 120014, KazakhstanLaboratory of Engineering Profile “Physical and Chemical Methods of Analysis”, Korkyt Ata Kyzylorda University, 29 Aiteke Bi Str., Kyzylorda 120014, KazakhstanLaboratory of Engineering Profile “Physical and Chemical Methods of Analysis”, Korkyt Ata Kyzylorda University, 29 Aiteke Bi Str., Kyzylorda 120014, KazakhstanLaboratory of Engineering Profile “Physical and Chemical Methods of Analysis”, Korkyt Ata Kyzylorda University, 29 Aiteke Bi Str., Kyzylorda 120014, KazakhstanPharmaceutical Chemistry and Centre of Excellence for Pharmaceutical Sciences, North-West University, Potchefstroom 2520, South AfricaPharmaceutical Technology Transfer Centre, Yaroslavl State Pedagogical University Named after K.D. Ushinsky, Respublikanskaya St., 108, 150000 Yaroslavl, RussiaInstitute of Chemistry, Saint Petersburg State University, Universitetskaya Nab., 7/9, 199034 Saint Petersburg, RussiaPharmaceutical Chemistry and Centre of Excellence for Pharmaceutical Sciences, North-West University, Potchefstroom 2520, South AfricaPharmaceutical Chemistry and Centre of Excellence for Pharmaceutical Sciences, North-West University, Potchefstroom 2520, South AfricaMonoamine oxidase (MAO) plays a key role in the pathogenesis of central nervous system disorders, and MAO inhibitors have been used in the treatment of depression and Parkinson’s disease. In the search for new classes of MAO inhibitors, the present study investigated a series of 1,2,4-oxadiazin-5(6<i>H</i>)-one derivatives. This study provides the first optimization of the reaction conditions for the condensation of amidoximes with alkyl 2-halocarboxylates to yield the desired 1,2,4-oxadiazin-5(6<i>H</i>)-ones. The results of the in vitro MAO inhibition studies showed that the 1,2,4-oxadiazin-5(6<i>H</i>)-ones were indeed inhibitors of human MAO with the most potent inhibition observed for <b>5f</b> (IC<sub>50</sub> = 0.900 µM) and <b>7c</b> (IC<sub>50</sub> = 0.371 µM). It was concluded that, with appropriate substitution, 1,2,4-oxadiazin-5(6<i>H</i>)-one derivatives would act as good potency MAO-B inhibitors and lead compounds for the development of antiparkinsonian drugs. In Parkinson’s disease, MAO-B inhibitors enhance central dopamine levels and reduce MAO-mediated production of hydrogen peroxide and resultant oxidative injury. This study represents one of few works to investigate synthetic approaches and biological activities of the 1,2,4-oxadiazin-5(6<i>H</i>)-one class of heterocycles.https://www.mdpi.com/1420-3049/29/23/5550monoamine oxidaseMAOoxadiazineinhibitionParkinson’s disease
spellingShingle Sofia I. Presnukhina
Valentina D. Kotlyarova
Anton A. Shetnev
Sergey V. Baykov
Rakhymzhan Turmanov
Nurbol Appazov
Rakhmetulla Zhapparbergenov
Leilya Zhussupova
Nurila Togyzbayeva
Stephanus J. Cloete
Mikhail K. Korsakov
Vadim P. Boyarskiy
Anél Petzer
Jacobus P. Petzer
Synthesis of 1,2,4-Oxadiazin-5(6<i>H</i>)-One Derivatives and Their Biological Investigation as Monoamine Oxidase Inhibitors
Molecules
monoamine oxidase
MAO
oxadiazine
inhibition
Parkinson’s disease
title Synthesis of 1,2,4-Oxadiazin-5(6<i>H</i>)-One Derivatives and Their Biological Investigation as Monoamine Oxidase Inhibitors
title_full Synthesis of 1,2,4-Oxadiazin-5(6<i>H</i>)-One Derivatives and Their Biological Investigation as Monoamine Oxidase Inhibitors
title_fullStr Synthesis of 1,2,4-Oxadiazin-5(6<i>H</i>)-One Derivatives and Their Biological Investigation as Monoamine Oxidase Inhibitors
title_full_unstemmed Synthesis of 1,2,4-Oxadiazin-5(6<i>H</i>)-One Derivatives and Their Biological Investigation as Monoamine Oxidase Inhibitors
title_short Synthesis of 1,2,4-Oxadiazin-5(6<i>H</i>)-One Derivatives and Their Biological Investigation as Monoamine Oxidase Inhibitors
title_sort synthesis of 1 2 4 oxadiazin 5 6 i h i one derivatives and their biological investigation as monoamine oxidase inhibitors
topic monoamine oxidase
MAO
oxadiazine
inhibition
Parkinson’s disease
url https://www.mdpi.com/1420-3049/29/23/5550
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