Expression and functional characterization of an anti-CD22 scFv targeting B-cell malignancies

Expression and functional characterization of an anti-CD22 scFv targeting B-cell malignancies

Background and purpose: Single-chain variable fragments (scFvs) offer advantages over full-length monoclonal antibodies in cancer therapy, including reduced size, lower production costs, and easier handling. However, Escherichia coli (E. coli) often leads to the formation and aggregation of inclusio...

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Main Authors: Monireh Gholizadeh, Shahriyar Abdoli, Shafieeh Mansoori, Arash Arashkia, Farhad Riazi-Rad, Amir Ali Hamidieh, Mohammad Nouri, Zahra Sharifzadeh
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2025-05-01
Series:Research in Pharmaceutical Sciences
Subjects:
cd22-scfv
e. coli expression system
imac purification
immunotherapy
on-column refolding
protein solubility
Online Access:https://journals.lww.com/10.4103/RPS.RPS_248_24
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