Evaluation of antimicrobial short sequence peptides derived from mackerel gill and intestine against gram-negative bacteria
Antimicrobial peptides (AMPs) are small peptides that play a crucial role in growth inhibiting of pathogenic microorganisms in many organisms including humans. Therefore, the study of those peptides is interesting in the applications in food and pharmaceutical industries. This research focuses on ma...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2025-01-01
|
| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2025/02/e3sconf_icome2025_02006.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841526744772771840 |
|---|---|
| author | Wonganu Benjamaporn Paenthong Mathaphon Roytrakul Sittiruk |
| author_facet | Wonganu Benjamaporn Paenthong Mathaphon Roytrakul Sittiruk |
| author_sort | Wonganu Benjamaporn |
| collection | DOAJ |
| description | Antimicrobial peptides (AMPs) are small peptides that play a crucial role in growth inhibiting of pathogenic microorganisms in many organisms including humans. Therefore, the study of those peptides is interesting in the applications in food and pharmaceutical industries. This research focuses on mackerel gills and intestines, which are low-value but protein-rich wastes from processing of mackerel. These wastes can be hydrolyzed to protein hydrolysate, which might contain several amino acids and bioactive peptides. From the previous results, the protein hydrolysate derived from mackerel gills and intestines wastes had inhibited the growth of gram-negative bacteria, Vibrio parahaemolyticus, Klebsiella pneumoniae, and Salmonella typhimurium. The objective of this research is to analyze and identify inhibitory mechanisms on the growth of salmonella when it was treated with small peptides by using Venn diagram and STITCH database. The obtained small peptides might affect the growth inhibitory activities, anticancer activity, gyrase activity, and anti-angiotensin activity. To increase antimicrobial activity of peptides, especially against Vibrio parahaemolyticus and Klebsiella pneumoniae, the model of peptides created from Pepwheel help predict for containing higher hydrophobicity and more symmetry in the helix structure might provide better antibacterial activity of the peptides. |
| format | Article |
| id | doaj-art-65652bf849c9476bb1e2ab9740e93f29 |
| institution | Kabale University |
| issn | 2267-1242 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj-art-65652bf849c9476bb1e2ab9740e93f292025-01-16T11:22:35ZengEDP SciencesE3S Web of Conferences2267-12422025-01-016020200610.1051/e3sconf/202560202006e3sconf_icome2025_02006Evaluation of antimicrobial short sequence peptides derived from mackerel gill and intestine against gram-negative bacteriaWonganu Benjamaporn0Paenthong Mathaphon1Roytrakul Sittiruk2Department of Biotechnology, Faculty of Applied Science, King Mongkut’s University of Technology North BangkokChulabhorn International College of Medicine, Thammasat UniversityFunctional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development AgencyAntimicrobial peptides (AMPs) are small peptides that play a crucial role in growth inhibiting of pathogenic microorganisms in many organisms including humans. Therefore, the study of those peptides is interesting in the applications in food and pharmaceutical industries. This research focuses on mackerel gills and intestines, which are low-value but protein-rich wastes from processing of mackerel. These wastes can be hydrolyzed to protein hydrolysate, which might contain several amino acids and bioactive peptides. From the previous results, the protein hydrolysate derived from mackerel gills and intestines wastes had inhibited the growth of gram-negative bacteria, Vibrio parahaemolyticus, Klebsiella pneumoniae, and Salmonella typhimurium. The objective of this research is to analyze and identify inhibitory mechanisms on the growth of salmonella when it was treated with small peptides by using Venn diagram and STITCH database. The obtained small peptides might affect the growth inhibitory activities, anticancer activity, gyrase activity, and anti-angiotensin activity. To increase antimicrobial activity of peptides, especially against Vibrio parahaemolyticus and Klebsiella pneumoniae, the model of peptides created from Pepwheel help predict for containing higher hydrophobicity and more symmetry in the helix structure might provide better antibacterial activity of the peptides.https://www.e3s-conferences.org/articles/e3sconf/pdf/2025/02/e3sconf_icome2025_02006.pdf |
| spellingShingle | Wonganu Benjamaporn Paenthong Mathaphon Roytrakul Sittiruk Evaluation of antimicrobial short sequence peptides derived from mackerel gill and intestine against gram-negative bacteria E3S Web of Conferences |
| title | Evaluation of antimicrobial short sequence peptides derived from mackerel gill and intestine against gram-negative bacteria |
| title_full | Evaluation of antimicrobial short sequence peptides derived from mackerel gill and intestine against gram-negative bacteria |
| title_fullStr | Evaluation of antimicrobial short sequence peptides derived from mackerel gill and intestine against gram-negative bacteria |
| title_full_unstemmed | Evaluation of antimicrobial short sequence peptides derived from mackerel gill and intestine against gram-negative bacteria |
| title_short | Evaluation of antimicrobial short sequence peptides derived from mackerel gill and intestine against gram-negative bacteria |
| title_sort | evaluation of antimicrobial short sequence peptides derived from mackerel gill and intestine against gram negative bacteria |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2025/02/e3sconf_icome2025_02006.pdf |
| work_keys_str_mv | AT wonganubenjamaporn evaluationofantimicrobialshortsequencepeptidesderivedfrommackerelgillandintestineagainstgramnegativebacteria AT paenthongmathaphon evaluationofantimicrobialshortsequencepeptidesderivedfrommackerelgillandintestineagainstgramnegativebacteria AT roytrakulsittiruk evaluationofantimicrobialshortsequencepeptidesderivedfrommackerelgillandintestineagainstgramnegativebacteria |