The Power of Old Hats: Rediscovering Inosine-EpPCR to Create Starting Libraries for Whole-Cell-SELEX

The Power of Old Hats: Rediscovering Inosine-EpPCR to Create Starting Libraries for Whole-Cell-SELEX

Shaking off the forgetfulness towards the methodological power of inosine-mediated error-prone PCR (epPCR), this study reintroduces an often-underappreciated method as a considerably powerful approach for generating aptamer libraries from a single decameric ATCG-repeat-oligonucleotide. The aim was t...

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Bibliographic Details
Main Authors: Grigory Bolotnikov, Ann-Kathrin Kissmann, Daniel Gruber, Andreas Bellmann, Roger Hasler, Christoph Kleber, Wolfgang Knoll, Frank Rosenau
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Biosensors
Subjects:
DNA aptamer
Cell-SELEX
biosensors
polyclonal library
Online Access:https://www.mdpi.com/2079-6374/15/7/448
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