Interferon epsilon is produced in the testis and protects the male reproductive tract against virus infection, inflammation and damage.
The testis is a reservoir for viruses that can cause persistent infection and adversely affect male reproductive health, an observation commonly attributed to deficiencies in inducible antiviral defence mechanisms. In this study, we demonstrate that interferon-epsilon (IFNε), a type I interferon ini...
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| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-12-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1012702 |
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| author | Rukmali Wijayarathna Eveline D de Geus Rosemary Genovese Linden J Gearing Georgie Wray-McCann Rajini Sreenivasan Hiba Hasan Monika Fijak Peter Stanton Daniela Fietz Adrian Pilatz Hans-Christian Schuppe Michelle D Tate Paul J Hertzog Mark P Hedger |
| author_facet | Rukmali Wijayarathna Eveline D de Geus Rosemary Genovese Linden J Gearing Georgie Wray-McCann Rajini Sreenivasan Hiba Hasan Monika Fijak Peter Stanton Daniela Fietz Adrian Pilatz Hans-Christian Schuppe Michelle D Tate Paul J Hertzog Mark P Hedger |
| author_sort | Rukmali Wijayarathna |
| collection | DOAJ |
| description | The testis is a reservoir for viruses that can cause persistent infection and adversely affect male reproductive health, an observation commonly attributed to deficiencies in inducible antiviral defence mechanisms. In this study, we demonstrate that interferon-epsilon (IFNε), a type I interferon initially discovered in female reproductive epithelia, is constitutively expressed by meiotic and post-meiotic spermatogenic cells, Leydig cells and macrophages in mouse testes. A similar distribution pattern was observed in human testes. Mice lacking IFNɛ were more susceptible to Zika virus-induced inflammation and damage of the testis and epididymis compared to wild-type mice. Exogenous IFNε treatment reduced the viral infection burden in cultured human testicular cells by inducing interferon-stimulated gene expression, and reducing inflammatory gene expression and cell damage. Treatment was more effective when administered prior to infection. These data indicate a critical role for constitutively-expressed IFNɛ in limiting viral infection and inflammatory damage in the male reproductive tract. |
| format | Article |
| id | doaj-art-653aa5223e4941eeb2eb9914ea809055 |
| institution | OA Journals |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-653aa5223e4941eeb2eb9914ea8090552025-08-20T02:36:58ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742024-12-012012e101270210.1371/journal.ppat.1012702Interferon epsilon is produced in the testis and protects the male reproductive tract against virus infection, inflammation and damage.Rukmali WijayarathnaEveline D de GeusRosemary GenoveseLinden J GearingGeorgie Wray-McCannRajini SreenivasanHiba HasanMonika FijakPeter StantonDaniela FietzAdrian PilatzHans-Christian SchuppeMichelle D TatePaul J HertzogMark P HedgerThe testis is a reservoir for viruses that can cause persistent infection and adversely affect male reproductive health, an observation commonly attributed to deficiencies in inducible antiviral defence mechanisms. In this study, we demonstrate that interferon-epsilon (IFNε), a type I interferon initially discovered in female reproductive epithelia, is constitutively expressed by meiotic and post-meiotic spermatogenic cells, Leydig cells and macrophages in mouse testes. A similar distribution pattern was observed in human testes. Mice lacking IFNɛ were more susceptible to Zika virus-induced inflammation and damage of the testis and epididymis compared to wild-type mice. Exogenous IFNε treatment reduced the viral infection burden in cultured human testicular cells by inducing interferon-stimulated gene expression, and reducing inflammatory gene expression and cell damage. Treatment was more effective when administered prior to infection. These data indicate a critical role for constitutively-expressed IFNɛ in limiting viral infection and inflammatory damage in the male reproductive tract.https://doi.org/10.1371/journal.ppat.1012702 |
| spellingShingle | Rukmali Wijayarathna Eveline D de Geus Rosemary Genovese Linden J Gearing Georgie Wray-McCann Rajini Sreenivasan Hiba Hasan Monika Fijak Peter Stanton Daniela Fietz Adrian Pilatz Hans-Christian Schuppe Michelle D Tate Paul J Hertzog Mark P Hedger Interferon epsilon is produced in the testis and protects the male reproductive tract against virus infection, inflammation and damage. PLoS Pathogens |
| title | Interferon epsilon is produced in the testis and protects the male reproductive tract against virus infection, inflammation and damage. |
| title_full | Interferon epsilon is produced in the testis and protects the male reproductive tract against virus infection, inflammation and damage. |
| title_fullStr | Interferon epsilon is produced in the testis and protects the male reproductive tract against virus infection, inflammation and damage. |
| title_full_unstemmed | Interferon epsilon is produced in the testis and protects the male reproductive tract against virus infection, inflammation and damage. |
| title_short | Interferon epsilon is produced in the testis and protects the male reproductive tract against virus infection, inflammation and damage. |
| title_sort | interferon epsilon is produced in the testis and protects the male reproductive tract against virus infection inflammation and damage |
| url | https://doi.org/10.1371/journal.ppat.1012702 |
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