CRISPRi screening reveals E. coli’s anaerobic-like respiratory adaptations to gentamicin: membrane depolarization by CpxR

CRISPRi screening reveals E. coli’s anaerobic-like respiratory adaptations to gentamicin: membrane depolarization by CpxR

ABSTRACT Bacterial genes serve diverse cellular functions and many affect fitness in response to environmental challenges. We employed CRISPR interference screening to investigate the fitness effect of each gene in Escherichia coli exposed to gentamicin, aiming to understand the cellular defense mec...

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Bibliographic Details
Main Authors: Donghui Choe, Eunju Lee, Yoseb Song, Sun Chang Kim, Ki Jun Jeong, Bernhard Palsson, Byung-Kwan Cho, Suhyung Cho
Format: Article
Language:English
Published: American Society for Microbiology 2025-07-01
Series:mSystems
Subjects:
CRISPR interference
gene essentiality
antibiotics
resistance
CpxR
Online Access:https://journals.asm.org/doi/10.1128/msystems.00353-25
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https://journals.asm.org/doi/10.1128/msystems.00353-25

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