An SDS-NaOH-based method to isolate genome of recombinant adeno-associated virus vectors for physical titer measurement.

Recombinant adeno-associated viruses (rAAVs) vectors are promising for their safety and sustained expression of genetic payloads across various tissues. These vectors consist of a protein capsid enclosing a 4.7 kb single-stranded DNA genome. Rapid and accurate determination of the physical titers of...

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Bibliographic Details
Main Authors: Xiangying Zhu, Keying Yang, Jinyan Xie, Xilin Feng, Tao Wu, Mengjun Hu, Haijian Wang, Chenghui Yu, Xiaomin Yu, Farhid Hemmatzadeh, Liqing Zhu, Linhua Zhang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0315921
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