CRISPR/Cas9-mediated simultaneous targeting of GmP34 and its homologs produces T-DNA-free soybean mutants with reduced allergenic potential

CRISPR/Cas9-mediated simultaneous targeting of GmP34 and its homologs produces T-DNA-free soybean mutants with reduced allergenic potential

Soybean (Glycine max L.) P34 (GmP34) is a prominent allergenic seed protein belonging to the papain-like cysteine protease family. To mitigate its allergenic potential, we implemented a CRISPR/Cas9-based genome editing strategy targeting GmP34 along with its two highly similar homologs, GmP34h1 and...

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Bibliographic Details
Main Authors: Dongwon Baek, Byung Jun Jin, Mi Suk Park, Ye Jin Cha, Tae Hee Han, Ye Na Jang, Su Bin Kim, Sang In Shim, Jong-Il Chung, Hyun Jin Chun, Min Chul Kim
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-08-01
Series:Frontiers in Plant Science
Subjects:
soybean
allergen
CRISPR/Cas9
genome editing
GmP34 homologs
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2025.1612747/full
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https://www.frontiersin.org/articles/10.3389/fpls.2025.1612747/full

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